Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator
A technology of plant growth regulators and metal derivatives, applied in the directions of plant growth regulators, plant growth regulators, chemicals for biological control, etc. Chemical properties and other issues, to achieve the effects of stable solution properties, reduced vigor, and excellent plant growth regulation activity
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Embodiment 1
[0031] Example 1: Chlorophyllase inhibitory activity (in vitro test) Accurately weigh 1.00 g of acetone powder made from fresh mung bean leaves, extract it with 25 mL of phosphate buffer (pH 7.0-7.5) for 4.5 hours, and centrifuge at 4°C for 10 min. The clear liquid is the chlorophyll enzyme liquid, the active enzyme concentration: 0.14 mmol / L, and the substrate chlorophyll concentration: 130 ppm. Determination of Chlorophyllase Inhibition Rate of Chlorophyllate Metal Derivatives Sample Solutions at 0.4ppm~25.0ppm (Gan Zhijun, Wang Xiaoyun, Zou Qi. Study on the Biochemical Kinetic Characteristics of Wheat Chlorophyllase. Northwest Botany, 2003, 23(5): 750 ~754.), and calculate IC 50 value, series sample IC 50 The results are shown in the table below
[0032] Chlorophyllase inhibitory activity IC of serial samples 50 *
[0033]
[0034] * : Substrate concentration 324ppm (0.36mM)
Embodiment 2
[0035] Example 2: Corn soaking germination rate and seedling growth time: April 2009; corn variety: Cuitian No. 1; sample: ferric chlorophyll chloride and ferric chlorophyll chloride; test method: 0.001ppm~1.0ppm sample solution The corn was soaked for 18 hours, and the water soaked group was the control (CK), and then transferred to the soil with the same soil quality and fertility for cultivation, and watered once every 2 days. Compared with the control after 10 days, examine the difference of its germination rate, plant height and main root length (all are average values), the results are shown in the following table
[0036] Comparison of germination rate and seedling growth of corn soaked seeds
[0037]
[0038]
[0039] Compared with CK, the germination rate, plant height and root length of each concentration in the experimental group were significantly different. The experimental group not only grew well, but also had a rich and developed root system, many lateral...
Embodiment 3
[0040] Example 3: Chlorophyllase inhibition experiment in vivo, selecting mung bean seeds with plump grains and the same size and soaking in 0.1 ppm solution of the novel plant growth regulator of the present invention for 4 hours, soaking the seeds at room temperature 28° C. to accelerate germination, selecting mung beans with consistent germination, and culturing in light 0.1ppm solution culture in the box, when 1-2 pairs of true leaves grow (about 8 days later), the leaves are used to make acetone powder, and the water culture is used as blank (inhibition rate: 0%), and the chlorophyllase activity of each treatment group is measured. , get the results in the table below
[0041]
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