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Tyrosine protein kinase receptor epha2 dominant epitope complex and its preparation method and application

A technology of tyrosine protein and dominant epitope, which is applied in chemical instruments and methods, hybrid peptides, drug combinations, etc., can solve problems such as complexes that have not yet been seen, and achieve effective induction of immune responses, good development and application prospects, inhibition of The effect of tumor growth

Inactive Publication Date: 2011-11-30
FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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Problems solved by technology

[0009] But so far, there is no report on the complex composed of EphA2 epitope, transmembrane sequence HIV-Tat49-57, endoplasmic reticulum retention signal sequence KDEL and LIGHT

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  • Tyrosine protein kinase receptor epha2 dominant epitope complex and its preparation method and application
  • Tyrosine protein kinase receptor epha2 dominant epitope complex and its preparation method and application
  • Tyrosine protein kinase receptor epha2 dominant epitope complex and its preparation method and application

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0028] 1. Preparation of EphA2 dominant epitope complex

[0029] 1. Prediction of EphA2 dominant epitope

[0030] A supermotif refers to a peptide motif in which the antigenic peptides accepted by HLA allotype molecules in the same HLA family, or even in different families, have the same and similar anchoring residues. The main anchor residues of the supermotif are the amino acid residues at the 2nd (P2) and 9th (...

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Abstract

The invention discloses a mesylate protein kinase receptor EphA2 dominant epitope compound and a preparation method and application thereof in preparing a therapeutic vaccine for tumor. The compound is prepared from EphA258-66 epitope fusion peptide, EphA2550-558 epitope fusion peptide and EphA2253-261 epitope fusion peptide in mol ratio of 1:1:1 and an LIGHT gene recombinant eukaryotic expression vector; the EphA258-66 / 550-558 / 253-261 epitope fusion peptides are respectively prepared from EphA258-66 / 550-558 / 253-261 epitopes and a transmembrane sequence HIV-Tat49-57, an endoplasmic reticulum retention signal sequence KDEL and a linker sequence AAY; the combination of the three EphA2 dominant epitopes can effectively overcome the deficiency of a single epitope on immune effect; the three epitope fusion peptides can enter the kytoplasm under the guide action of the transmembrane sequence, target the endoplasmic reticulum under the action of the endoplasmic reticulum retention signal sequence, and efficiently enter an MHC-I (major histocompatibility complex-class I) antigen presenting route; and the LIGHT gene recombinant eukaryotic expression vector can express LIGHT protein in vivoand play a role of a molecular adjuvant to promote the immune response of epitope specific T cells.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an antigen epitope complex, in particular to a dominant epitope complex of tyrosine protein kinase receptor EphA2, and also relates to a preparation method and application of the dominant epitope complex. Background technique [0002] A lot of progress has been made in the treatment of malignant tumors, but the minimal residual lesions are still difficult to be completely removed by current radiotherapy, chemotherapy, surgery and other programs. Applying tumor immunotherapy to stimulate the body's immune surveillance system to eliminate residual tumor cells may completely cure the tumor. Therefore, the development of tumor vaccines has become one of the current research hotspots. [0003] In view of the decreased immunogenicity of tumor antigens in the body, resulting in insufficient activation of specific cellular immunity and peripheral immune tolerance, the general idea of ​​tumor vac...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/39C07K19/00A61P35/00
Inventor 陈宏颉王守森郑兆聪袁邦青王如密高进喜赵琳
Owner FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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