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Method for measuring refractive index of microsphere or medium and application thereof

A technology of refractive index and refractive index ratio, which is applied in the field of optical measurement, can solve difficult problems such as rapid, real-time, and in-situ accurate measurement of refractive index, and achieve the effect of simple method, high precision and accurate measurement

Active Publication Date: 2011-11-23
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For samples such as living cells, because the cells and their surrounding media themselves will move, it is difficult for existing methods to quickly, real-time, and in-situ accurately measure the internal local refractive index of heterogeneous media and living organisms.

Method used

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  • Method for measuring refractive index of microsphere or medium and application thereof
  • Method for measuring refractive index of microsphere or medium and application thereof
  • Method for measuring refractive index of microsphere or medium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Measurement of the refractive index of polystyrene microspheres with water as the medium: a small amount of water (n 1 =1.3345) was dropped on a microscope slide, and then a polystyrene microsphere was immersed in water, and then the slide was placed under a phase-contrast microscope and imaged by its digital imaging system. After correcting the spatial scale magnification of the measurement condition with the image analysis software IPP, the obtained microsphere phase contrast image (see figure 1 (a)) Carry out the measurement of its radius R and the center bright circle radius r, obtain 2R=36.83±0.06 micron respectively, 2r=25.59±0.07 micron, adopt equation (1) to calculate polystyrene microsphere refractive index 1.4330±0.0002 .

Embodiment 2

[0038] Measurement of the refractive index of polystyrene microspheres with ethanol as the medium: a small amount of ethanol (n 1 =1.3628) was dropped on a microscope slide, and then a polystyrene microsphere was immersed in ethanol, and then the slide was placed under a phase-contrast microscope and imaged by its digital imaging system. After correcting the spatial scale magnification of the measurement condition with the image analysis software IPP, the obtained microsphere phase contrast image (see figure 1 (b)) Carry out the measurement of its radius R and the central bright circle radius r, obtain 2R=36.88 ± 0.06 micron respectively, 2r=31.61 ± 0.07 micron, adopt equation (1) to calculate polystyrene microsphere refractive index 1.4321 ± 0.0002 .

Embodiment 3

[0040] Measure the refractive index of polystyrene microspheres with a mixture of glycerol and water as the medium: mix a small amount of glycerol and water to form a 1:1 mixture, and measure the refractive index n with an Abbe refractometer 1 =1.4055, then drop the mixed solution on a microscope slide, and then dip a polystyrene microsphere into it. The slide was placed under a phase contrast microscope and imaged by its digital imaging system. After correcting the spatial scale magnification of the measurement condition with the image analysis software IPP, the obtained microsphere phase contrast image (see figure 1 (c)) Carry out the measurement of its radius R and the central bright circle radius r, obtain 2R=36.85 ± 0.06 micron respectively, 2r=34.27 ± 0.06 micron, adopt equation (1) to calculate polystyrene microsphere refractive index 1.4359 ± 0.0002 .

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Abstract

The invention provides a method for measuring refractive index of a microsphere or a medium. The method comprises the following steps of: imaging the microsphere immersed in the medium with lower refractive index under parallel light irradiation; measuring the ratio of the radius R of a microsphere image to the radius r of the central bright round of the microsphere image; accurately measuring the refractive index of the microsphere under the condition of knowing the refractive index of the medium; and accurately measuring the refractive index of the medium under the condition of knowing the refractive index of the microsphere, wherein the microsphere is an optically transparent mini sphere and the medium is an optically transparent substance. The method is simple and convenient, and easy to operate. The relative refractive index can be obtained only by shooting the image of the microsphere, measuring the ratio of the radius r of the central bright round of the microsphere image to the radius R of the microsphere, and using an equation (A). The method can be used for measuring the refractive index or the concentration or the temperature change of the mixed fluid at different special points, the refractive index of a phase change critical point or the dynamic change of concentration distribution, the molecular structure and the concentration change or the temperature change in an organism and the like.

Description

technical field [0001] The invention relates to optical measurement technology, in particular to a method for measuring the refractive index of a microsphere or a medium and its application. Background technique [0002] Light-transmitting microsphere particles, often used as a sensor in many aspects, can be used in biomedical research as a tool for carrying or testing various biomedical substances, or as a model for studying the optical properties of cells and tissues. It can be used as a benchmark for calibrating scientific instruments, and can also be used as an energy carrier or sensor for electromagnetic waves, as well as a high-Q reactor. In all of these applications, it is necessary to measure the refractive index of the microspheres. On the other hand, there is also a strong demand for real-time and on-site measurement and analysis of the local refractive index in heterogeneous media and living organisms, because the refractive index is usually in a certain linear p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/41A61B5/00A61B5/01
Inventor 黄耀熊岳粮跃
Owner JINAN UNIVERSITY
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