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Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops

A safety evaluation and micro-plate technology, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of heavy workload, large errors, incompleteness, etc., and achieves simple methods, high sensitivity, and resolution. strong effect

Inactive Publication Date: 2011-11-23
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a Biolog ECO micro-plate technology applied to the evaluation of the soil micro-ecological safety of transgenic crops, especially to provide a quick and sensitive evaluation of the effect of transgenic plants on the function of rhizosphere soil microbial communities In order to solve the problems of heavy workload, large errors, and incompleteness of traditional methods, the Biolog ECO microplate technology was successfully applied to the evaluation of the microecological safety of rhizosphere soil by transgenic Bt crops

Method used

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  • Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops
  • Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops
  • Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops

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Experimental program
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Embodiment 1

[0038] Taking the transgenic rice as the research object, the biolog ECO technology was used to analyze the rhizosphere soil microorganisms of the transgenic rice and its non-transgenic rice parents at different growth stages (such as seedling stage, tillering, jointing, heading, and maturity) under pot conditions. Changes in the level of carbon source utilization.

[0039] The Biolog ECO technology of the present invention uses the BIOLOG Ecoplate TMMicroplate (BIOLOG Inc., USA), this ecological plate is a 96-well reaction microplate with three repetitions, except that the three control wells A1 are only equipped with tetraazolium redox dyes and some nutrients, the rest The wells are each loaded with different single carbon substrates. This Biolog ECO technology analyzes and evaluates the physiological and metabolic characteristics of soil microorganisms through the distribution of carbon source substrates in 96 wells.

[0040] (1) Sample preparation

[0041] Weigh 1 g of ...

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Abstract

The invention discloses a Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops. The Biolog ECO micro-plate technique comprises the following steps: (1) collecting rhizosphere soil samples of the GM crops and non-GM parent crops during different childbearing periods; (2) performing Biolog ECO experiments; (3) calculating average well color development (AWCD) and diversity indexes; and (4) performing principal component analysis (PCA). The Biolog ECO micro-plate technique can be used for evaluating the influence of the GM crops on functions of rhizosphere soil microbial communities so as to evaluate the potential security risk of the GM crops for a rhizosphere soil microecosystem.

Description

technical field [0001] The invention relates to a Biolog ECO micro-plate technology applied to soil micro-ecological safety evaluation of transgenic crops, which belongs to the field of safety evaluation of genetically modified organisms and microbial ecology. Background technique [0002] Since the success of the world's first transgenic plant in 1983, the use of genetic engineering to improve and optimize crops has made great achievements in increasing yield, improving the nutritional content of agricultural products, shortening the breeding cycle, and enhancing resistance to diseases and insect pests. Many genetically modified crops such as corn, cotton, soybean, etc. have entered commercial production. However, whether these crops that have been transferred with exogenous genes will bring unpredictable risks to people is controversial, and the safety of genetically modified crops is also a hot spot of concern to the international community. [0003] Soil is an important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 唐雪明魏曼曼朱宏程凯王金斌赵凯
Owner SHANGHAI ACAD OF AGRI SCI
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