Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops
A safety evaluation and micro-plate technology, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of heavy workload, large errors, incompleteness, etc., and achieves simple methods, high sensitivity, and resolution. strong effect
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[0038] Taking the transgenic rice as the research object, the biolog ECO technology was used to analyze the rhizosphere soil microorganisms of the transgenic rice and its non-transgenic rice parents at different growth stages (such as seedling stage, tillering, jointing, heading, and maturity) under pot conditions. Changes in the level of carbon source utilization.
[0039] The Biolog ECO technology of the present invention uses the BIOLOG Ecoplate TMMicroplate (BIOLOG Inc., USA), this ecological plate is a 96-well reaction microplate with three repetitions, except that the three control wells A1 are only equipped with tetraazolium redox dyes and some nutrients, the rest The wells are each loaded with different single carbon substrates. This Biolog ECO technology analyzes and evaluates the physiological and metabolic characteristics of soil microorganisms through the distribution of carbon source substrates in 96 wells.
[0040] (1) Sample preparation
[0041] Weigh 1 g of ...
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