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Wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of wheat rhizosphere azotobacter

A technology of fungi and bacterial agents, applied in the field of nitrogen-fixing bacteria in the wheat rhizosphere, to achieve broad application prospects, high nitrogenase activity, and good inoculation effects

Inactive Publication Date: 2013-03-20
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck restricting the development of my country's microbial fertilizer industry is the selection of high-efficiency strains

Method used

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  • Wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of wheat rhizosphere azotobacter
  • Wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of wheat rhizosphere azotobacter
  • Wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of wheat rhizosphere azotobacter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1, the isolation and identification of wheat rhizosphere nitrogen-fixing bacteria GD641

[0046] 1. Enrichment and isolation of wheat rhizosphere nitrogen-fixing bacteria GD641

[0047] Take 10g of soil sample (collected from Inner Mongolia, China) and shake it in 90ml of sterile water for 20 minutes to make a turbid solution. Take 5ml and put it into 30ml of nitrogen-fixing bacteria enrichment culture medium ACCC55, 100rpm, 28℃ for shaking culture, 72h Then replace with fresh medium to continue culturing. The nitrogen-fixing spores were isolated after repeating the culture for 3 times. Draw 1ml of the above-mentioned nitrogen-fixing bacteria enrichment culture and put it into 9ml sterile water to make 10 -1 Dilution, continue to dilute to make 10 -2 、10 -3 、10 -4 、10 -5 Bacterial suspensions of dilutions were heated in boiling water at 100°C for 10 minutes, and after cooling, 0.1ml of each dilution was spread on a nitrogen-fixing bacteria isolation medium...

Embodiment 2

[0076] Example 2, Determination of Nitrogenase Activity of Paenibacillus sp. GD641 CGMCC No.5042

[0077] The Paenibacillus sp. (Paenibacillus sp.) GD641 CGMCC No.5042 obtained in Example 1 is carried out nitrogenase activity assay, and concrete method is as follows: in 15 * 150mm screw-top glass tubes, add 5ml improved nitrogen fixation medium and make inclined plane , inoculated with nitrogen-fixing bacteria and cultured at 28°C. Azotobacter chroococcum (Azotobacter chroococcum) ACCC11103, which is commonly used in the production of microbial fertilizers, was used as the positive control, and the blank slant without inoculation was used as the negative control, with 3 replicates. After culturing for 72 hours, replace the rubber stopper, inject acetylene gas to make the final concentration 10%, seal it with medical adhesive tape, continue culturing for 72 hours, take 100 μl of reaction gas, measure the amount of ethylene produced by gas chromatography, and calculate Paenibaci...

Embodiment 3

[0080] Embodiment 3, Paenibacillus sp. (Paenibacillus sp.) GD641 CGMCC No.5042 antagonizes pathogenic fungus inhibition rate determination

[0081] Adopt the two-point confrontation method to carry out the antagonism pathogenic fungus bacteriostatic rate measurement to the Paenibacillus sp. (Paenibacillus sp.) GD641 CGMCC No.5042 that embodiment 1 obtains, concrete operation is as follows: on the PDA plate apart from two points of center 2cm Inoculate crops with Gibberella zeae (or Sclerotinia sclerotiorum, or Verticillium dahliae) and Paenibacillus sp. GD641 CGMCC No.5042 on each screening treatment Three repetitions were performed, and the plate inoculated with pathogenic fungi but not nitrogen-fixing bacteria was used as a control. Incubate at a constant temperature at 28°C, and after 15 days, use a millimeter scale to measure the colony radius r of the pathogenic fungi on the confrontation plate along the direction of the tested Paenibacillus sp. GD641 CGMCC No.5042 1 , a...

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Abstract

The invention discloses a wheat rhizosphere azotobacter for antagonism of various pathogenic fungi and application of the wheat rhizosphere azotobacter. The wheat rhizosphere azotobacter is Paenibacillus sp. GD641, and has the collection number of CGMCC No.5042 in China General Microbiological Culture Collection Center. The Paenibacillus sp. GD641 with the collection number of CGMCC No.5042 has effective antagonism on Gibberella zeae, Sclerotinia sclerotiorum and Verticillium dahlia of crops, and has wide application prospect in microbial azotobacter and biological organic fertilizer production.

Description

technical field [0001] The invention relates to wheat rhizosphere nitrogen-fixing bacteria that antagonize various pathogenic fungi and uses thereof, in particular to a bacterium that can antagonize crop scab, sclerotinia and verticillium dahliae and has the function of efficiently nitrogen-fixing wheat rhizosphere. Background technique [0002] Nitrogen fertilizer is one of the most important means of production in agricultural production. The production of chemical nitrogen fertilizer consumes a large amount of energy, accounting for 70% to 80% of the total production cost. In recent years, energy has become increasingly tense, and the price of coal for chemical fertilizer production in my country has soared, leading to an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response. In addition, chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food security in my country, but...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/00A01N63/02A01P3/00C05F11/08C12R1/01
Inventor 孙建光徐晶胡海燕高淼
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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