Preparation method and application of two active flavonoid glycosides in okra fruits

A technology of okra tender fruit and flavonoid glycosides is applied in the field of preparation of two active flavonoid glycosides in okra fruit, and achieves the effects of simple preparation process, easy industrialized production, significant antioxidant and bacteriostatic activities

Inactive Publication Date: 2011-11-23
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the isolation, identification and biological activity of flavonoid glycosides from okra fruit

Method used

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  • Preparation method and application of two active flavonoid glycosides in okra fruits
  • Preparation method and application of two active flavonoid glycosides in okra fruits
  • Preparation method and application of two active flavonoid glycosides in okra fruits

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Embodiment 1

[0043] 1) Processing of raw materials: Take the tender okra fruit, cut it into slices and dry it in the sun or air, and set aside;

[0044] 2) Percolation extraction: put the dried flaky okra fruit into a percolation cylinder, soak it with 70% hydrous ethanol equivalent to 1 to 2 times the amount of dried okra fruit for 5 hours, put it into the percolation barrel, and use the equivalent amount of okra dried fruit 14 times the amount of 70% aqueous ethanol for percolation extraction, adjust the flow rate, and collect the percolation extract at a flow rate of 10 mL / min;

[0045] 3) Flash concentration: The percolation extraction solution was flash concentrated using a vacuum film concentration device under the conditions of a water bath temperature of 65 °C and a vacuum degree of 0.095 Mpa, and the feed rate of the liquid to be concentrated was adjusted at a rate of 250 mL min -1 The flow rate is repeatedly concentrated to obtain a concentrated solution containing 0.5 to 1.0 gra...

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Abstract

Belonging to the technical field of extraction and separation, the invention relates a preparation method and application of two active flavonoid glycosides in okra fruits. With okra fruits as the raw materials, the method comprises the steps of: transferring out polysaccharide and pectin through percolation extraction, flash evaporation and condensation, extraction and decolouration, alcohol precipitation, carrying on vacuum condensation, separating enrichment by a macroporous adsorption resin column, then conducting separation by combing the gel resin column chromatography technology, thus obtaining two monomer compounds of 5, 7, 3', 4'-tetrahydroxy-4''-O-methyl-3-O-beta-D-glucose flavonoid glycoside and 5, 7, 3', 4'- tetrahydroxy-3-O-[beta-D-glucosyl-(1-2)]-beta-D-glucose flavonoid glycoside, with extraction rates of 3.62%-8.66% and 8.01%-12.98% respectively. The purity of the two compounds is both above 95%. The method of the invention has simple process and low production cost, and the two flavonoid glycosides are characterized by good antioxidation and bacteriostatic activity.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation, in particular to a preparation method and application of two active flavonoid glycosides in okra fruit. Background technique [0002] Okra is a plant of the Malvaceae genus Okra, which is cultivated in the north and south of my country. Okra has been designated as the first choice vegetable for athletes and health food for the elderly in many countries. It is called "plant Viagra" and "green ginseng" in the United States, Japan and other countries. It is an excellent dietary and health vegetable with high nutritional value. . [0003] Okra contains flavonoids, polysaccharides, pectin, trace elements, amino acids and other functional ingredients, which have anti-fatigue, enhance human immunity, protect the liver and strengthen the kidney, anti-aging, lower blood sugar, help digestion, clear fire and improve eyesight, and strengthen the stomach The role of moistening the intesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P39/06A61P31/00A23L1/29A23L33/00
Inventor 袁珂廖海兵殷明文林晓春
Owner ZHEJIANG FORESTRY UNIVERSITY
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