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High density fermentation medium for feeding lactobacillus, and corresponding fermentation method

A technology of high-density fermentation and fermentation medium, which is applied in the field of high-density fermentation medium and fermentation of lactic acid bacteria for feed, and can solve the problems of Lactobacillus salivarius difficult to achieve high-density fermentation, high-density fermentation difficult to achieve, and insufficient nutrients. Achieve the effects of shortening the production cycle, reducing separation costs and improving production efficiency

Active Publication Date: 2011-10-26
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lactobacillus salivarius is still difficult to achieve high-density fermentation. The main problem that is difficult to achieve is the fermentation medium and its fermentation method. The lack of nutrients and the accumulation of metabolites (mainly lactic acid) in the late fermentation period inhibit the growth of bacteria, making it difficult to achieve high-density fermentation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 fermentation medium and fermentation comparison

[0036] 1. The components and content of Lactobacillus salivarius high-density fermentation medium are:

[0037] Glucose: 20g / L, tryptone: 10g / L, beef extract: 10g / L, yeast powder: 5g / L, K 2 HPO 4 : 2g / L, CH 3 COONa: 5g / L, sodium citrate: 2g / L, MgSO 4 : 0.2g / L, FeSO 4 : 0.1g / L, MnSO 4 : 55mg / L.

[0038] 2. Medium preparation method:

[0039] (1) Weigh each component according to the above-mentioned content, dissolve with distilled water, wherein glucose is dissolved separately;

[0040] (2) Glucose is sterilized separately at 115°C for 20 minutes, and other components are put into a fermenter and sterilized at 121°C for 20 minutes;

[0041] (3) Before inoculation, glucose is added into the fermenter through aseptic operation, stirred and mixed evenly, and the obtained product is obtained.

[0042] 3. Comparison between high-density fermentation and existing technology fermentation ...

Embodiment 2

[0056] Preparation and fermentation comparison of embodiment 2 fermentation medium

[0057] 1. The components and contents of Lactobacillus salivarius high-density fermentation medium are:

[0058] Glucose: 25g / L, tryptone: 10g / L, beef powder: 15g / L, yeast powder: 10g / L, K 2 HPO 4 : 2g / L, CH 3 COONa: 7g / L, sodium citrate: 3g / L, MgCl 2 : 0.2g / L, FeCl 2 : 0.1g / L, MnSO 4 : 50mg / L.

[0059] 2. Medium preparation method:

[0060] (1) Weigh each component according to the content, dissolve with distilled water, wherein glucose is dissolved separately;

[0061] (2) 10 pounds of glucose was sterilized separately for 20 minutes, and other components were sterilized for 20 minutes at 15 pounds in a fermenter;

[0062] (3) Before inoculation, glucose is added into the fermenter through aseptic operation, stirred and mixed evenly, and the obtained product is obtained.

[0063] 3. Comparison between high-density fermentation and existing technology fermentation

[0064] 3.1 The m...

Embodiment 3

[0077] Preparation and fermentation comparison of embodiment 3 fermentation medium

[0078] 1. The components and contents of Lactobacillus salivarius high-density fermentation medium are:

[0079] Glucose: 30g / L, tryptone: 15g / L, beef extract: 6g / L, yeast powder: 6g / L, K 2 HPO 4 : 3g / L, CH 3 COONa: 8g / L, sodium citrate: 4g / L, (NH 4 ) 2 Fe(SO 4 ) 2 : 0.2g / L, MgSO 4 : 0.4g / L, MnSO 4 : 45mg / L.

[0080] 2. Medium preparation method:

[0081] (1) Weigh each component according to the content, dissolve with distilled water, wherein glucose is dissolved separately;

[0082] (2) Glucose is sterilized separately at 115°C for 20 minutes, and other components are put into a fermenter and sterilized at 121°C for 20 minutes;

[0083] (3) Before inoculation, glucose is added into the fermenter through aseptic operation, stirred and mixed evenly, and the obtained product is obtained.

[0084] 3. Comparison between high-density fermentation and existing technology fermentation

...

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Abstract

The invention belongs to the technical field of lactobacillus cultivation, and relates to a high density fermentation medium for feeding lactobacillus, and a corresponding fermentation method. According to the present invention, a strategy of employing pH feedback to control glucose feeding is adopted, and the density of lactic acid in fermentation broth is reduced, such that feedback inhibition effect of lactic acid is released. With the fermentation medium and the fermentation method provided by the present invention, thalline density of cultivated saliva lactobacillus is substantially improved comparing to that obtained with common fermentation method, live bacteria content is no less than 10<10>cfu / ml, which is increased by 50 times or more than the content of live bacteria cultivatedwith a common MRS medium, such that high density fermentation of saliva lactobacillus is realized. Therefore, separating cost of biomass can be reduced, production period can be shortened, productioncost can be reduced, and production efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria cultivation, and relates to a high-density fermentation medium for feeding lactic acid bacteria and a fermentation method thereof. Background technique [0002] Lactic acid bacteria are normal bacteria commonly found in the digestive tract of animals. The history of using this type of bacteria to develop probiotics is the earliest. This bacteria has been widely used in fermented food, animal feed and health food. Lactobacillus salivarius (Lactobacillus salivarius) is a kind of lactic acid bacteria. It can inhibit the proliferation of pathogenic bacteria in the host body by producing lactic acid, bacteriocin and other antibacterial substances, maintain the balance of digestive tract microflora, and reduce diarrhea caused by Escherichia coli and Salmonella. Improve body health. [0003] The fermentation density of the bacteria in the fermentation liquid obtained by the traditional stat...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/225A23K10/18
Inventor 汪攀张军孙晓雯闫轶洁王安如杨欣
Owner BEIJING DABEINONG TECH GRP CO LTD
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