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Method for measuring content of verbascoside in callicarpa nudiflora preparation

A technology of acteoside and nude flower purple pearl, which is applied in the direction of anti-inflammatory agents, anti-virus agents, anti-viral agents, etc., can solve the problems of inaccurate quantification and no method for measuring the content of acteoside, so as to ensure clinical effect, small error, The effect of raising quality standards

Active Publication Date: 2013-03-20
JIANGXI POZIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly granules, capsules, tablets, dispersible tablets, mixtures, soft capsules, suppositories, etc. in the market. The quality standards of the above-mentioned preparations are only part of the preparations. The content of total flavonoids has been determined by ultraviolet spectroscopy Photometric method, the quantification is very inaccurate, there is no method for the determination of the content of verbascoside in the nude flower purple pearl preparation

Method used

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  • Method for measuring content of verbascoside in callicarpa nudiflora preparation
  • Method for measuring content of verbascoside in callicarpa nudiflora preparation
  • Method for measuring content of verbascoside in callicarpa nudiflora preparation

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Effect test

Embodiment 1

[0054] The assay method of the content of actascoside in the nude flower purple pearl preparation adopts the high performance liquid chromatography to measure the content of the verbascoside in the nude flower purple pearl preparation test solution.

[0055] Chromatographic conditions and system suitability Octadecylsilane-bonded silica gel was used as filler, acetonitrile-0.1% acetic acid solution (16:84) was used as mobile phase, the detection wavelength was 332nm, and the number of theoretical plates should not be less than 4000 based on the peak of verbascoside.

[0056] Preparation of reference solution Take an appropriate amount of verbascoside reference substance, weigh it, add 50% methanol to make a solution containing 40mg per 1ml, and get it.

[0057] Preparation of the test solution Take 2 bags of Nudehuazizhu granules, grind them finely, take 0.7g, weigh them, put them in a 50ml stoppered Erlenmeyer flask, add 25ml of 50% methanol, weigh them, and treat ...

Embodiment 2

[0061] The assay method of the content of actascoside in the nude flower purple pearl preparation adopts the high performance liquid chromatography to measure the content of the verbascoside in the nude flower purple pearl preparation test solution.

[0062] Chromatographic conditions and system suitability Octadecylsilane-bonded silica gel is used as filler, methanol-0.1% acetic acid solution (18:82) is used as mobile phase, the detection wavelength is 322nm, and the number of theoretical plates should not be less than 4000 based on the peak of verbascoside.

[0063] Preparation of reference solution Take an appropriate amount of verbascoside reference substance, weigh it, add 50% methanol to make a solution containing 40mg per 1ml, and get it.

[0064] Preparation of the test solution Take 2 bags of nudihuazizhu granules, grind them finely, take 0.8g, weigh them, put them in a 50ml stoppered Erlenmeyer flask, add 50ml of 30% methanol, weigh them, treat them with u...

Embodiment 3

[0068] The assay method of the content of actascoside in the nude flower purple pearl preparation adopts the high performance liquid chromatography to measure the content of the verbascoside in the nude flower purple pearl preparation test solution.

[0069] Chromatographic conditions and system suitability Octadecylsilane-bonded silica gel was used as filler, acetonitrile-0.3% phosphoric acid solution (10:90) was used as mobile phase, the detection wavelength was 345nm, and the number of theoretical plates should not be less than 4000 based on the verbascoside peak.

[0070] Preparation of reference solution Take an appropriate amount of verbascoside reference substance, weigh it, add 50% methanol to make a solution containing 40mg per 1ml, and get it.

[0071] Preparation of the test solution Take 10 pieces of Nudehua Zizhu Tablets, grind them finely, take 0.5g, weigh them, put them in a 50ml Erlenmeyer flask with a stopper, add 15ml of 100% methanol, weigh them, ...

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Abstract

The invention discloses a method for measuring the content of verbascoside in a callicarpa nudiflora preparation, relating to a method for measuring the content of effective ingredients in the callicarpa nudiflora preparation. A high performance liquid chromatography (HPLC) is used for measuring the content of verbascoside in the test article solution of the callicarpa nudiflora preparation. Chromatographic conditions and system suitability are as follows: octadecylsilane chemically bonded silica is used as a filler, a flowing phase contains an organic solvent and an acidic solution, the volume percent of the organic solvent in the flowing phase is 10-30%, the volume percent of the acidic solution in the flowing phase is 70-90%, the concentration of the acidic solution is 0-0.3%, and the detection wavelength is 322-345nm. The method disclosed by the invention can be used for accurately and effectively measuring the content of verbascoside in the callicarpa nudiflora preparation, has a small error, can further improve the quality standard of the callicarpa nudiflora preparation, and can ensure the quality and clinical effect of the callicarpa nudiflora preparation.

Description

technical field [0001] The invention relates to a method for determining the content of active ingredients in the nudibranch preparation, in particular to a method for determining the content of verbascoside in the nude flower preparation. Background technique [0002] Callicarpanudiflorae is a small shrub plant of Verbenaceae, which has the functions of hemostasis, antibacterial, astringent, detoxification, analgesia, and promotion of healing. Its antibacterial and antiviral effects, especially have different inhibitory effects on Staphylococcus aureus, typhoid bacillus, and pneumococcus, among which the antibacterial effect on Staphylococcus aureus and typhoid bacillus is the strongest. Nadihuazizhu can also inhibit the permeability of capillaries, and has obvious anti-inflammatory effect on early inflammation exudation and swelling. The main components of Nadiflower are flavonoid glycosides, condensed tannins, neutral resins, phenols, polysaccharides, hydroxyl compounds,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06A61K36/85A61P7/04A61P31/04A61P39/02A61P29/00A61P17/02A61P31/12A61P9/14A61K31/7028
Inventor 龚志南吴永忠余宝平余显英李旭张荷英
Owner JIANGXI POZIN PHARMA
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