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Medium for rapid breeding and propagation of fraxinus americana tissue culture and application thereof

A technology of proliferation medium and tissue culture, applied in the field of forest tissue culture, can solve problems such as unfavorable large-scale industrial production, large consumption of agar and sucrose, shortened proliferation and transfer cycle, etc. The effect of short connection period and fast proliferation rate

Inactive Publication Date: 2011-09-28
LIAONING FORESTRY SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to overcome the low proliferation rate of the existing Ash tissue culture, the long propagation and transfer cycle, the large consumption of agar and sucrose, the high cost of the medium, and the need for large-scale industrial production, the purpose of the present invention is to provide a suitable American white wax mature seed embryo induction sterile seedling stem section tissue culture rapid proliferation medium and its application, make the white wax tree tissue culture proliferation rate fast, the proliferation transfer period shortened, the agar consumption is small, the cost of the medium is reduced, and it is beneficial to large-scale The need for large-scale factory production

Method used

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  • Medium for rapid breeding and propagation of fraxinus americana tissue culture and application thereof
  • Medium for rapid breeding and propagation of fraxinus americana tissue culture and application thereof
  • Medium for rapid breeding and propagation of fraxinus americana tissue culture and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1) Preparation of induction medium, sterilization and acquisition of sterile materials

[0028] MS is the basic medium, add agar 6.0g L -1 , white sugar 30g·L -1 1. Adjust the pH value of the culture medium to 5.8-5.9, and divide into 100ml glass Erlenmeyer flasks, about 40ml per bottle. The medium and culture vessels were sterilized by high pressure moist heat disinfection. Put the subpackaged culture medium, wrapped culture container, tweezers, scalpel and other experimental equipment in an autoclave, 121°C, 1.1kg.cm -3 Sterilize for 20 minutes.

[0029]Select the plump American white wax seeds, peel off the seed shell, rinse with water for 2-3 hours, soak in 70% alcohol on the ultra-clean workbench for 30 seconds, rinse with sterile water for 2-3 times, and use 0.1% HgCl 2 Disinfect for 8 minutes, rinse with sterile water 3 to 4 times, cut out the seed embryos, inoculate them in MS medium, and place them in the culture room.

[0030] 2) Proliferation medium prep...

Embodiment 2

[0037] The acquisition of this example material and condition are identical with embodiment 1 method, differ from embodiment 1 in that:

[0038] 2) Preparation of proliferation medium and sterilized MS as the basic medium, adding 0.2mg·L -1 Difenuron, 0.2mg·L -1 6-benzylaminoadenine (6-BA) and 0.2mg L -1 Thiadiazole urea (TDZ), combined into three types of medium, and then add agar 3.5g L -1 , sucrose 30g·L -1 , Naphthaleneacetic acid 0.2mg·L -1 , adjust the pH value of the medium to 5.8-5.9, and distribute it in 100ml glass Erlenmeyer flasks, about 40ml per bottle. Sterilization method is the same as step "1" of example 1.

[0039] 3) When the sterile seedlings grow to about 3-4 cm, cut out the single-bud stem section, inoculate it in the proliferation medium, observe the growth after 4 weeks, and add 0.2 mg·L -1 In the culture medium of furdifenuron, the multiplication coefficient was up to 6.4, the average bud height was 2.16cm, and the proliferating seedlings grew vi...

Embodiment 3

[0043] The acquisition of this example material and condition are identical with embodiment 1 method, differ from embodiment 1 in that:

[0044] WPM is the basic medium, add agar 4.0g L -1 , white sugar 40g·L -1 , 0.3mg L -1 , Indolebutyric acid 0.3mg·L -1 , adjust the pH value of the medium to 5.8-5.9, and distribute it in 100ml glass Erlenmeyer flasks, each bottle is about 40ml. Sterilization method is the same as step "1" of example 1.

[0045] 3) When the sterile seedlings grow to 3-4 cm, cut out the single-bud stem section, inoculate it in the proliferation medium, observe the growth after 4 weeks, the proliferation coefficient reaches 6.8, the average bud height is 1.96 cm, and the multiplication seedlings grow robustly.

[0046] The proliferation medium of the present invention is effective for seed embryo induction of stem segment proliferation of sterile seedlings, wherein MS medium is added with 0.15mg L -1 , Naphthaleneacetic acid 0.2mg·L -1 , white sugar 30g·...

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Abstract

The invention relates to forest tissue culture, and specifically relates to a medium for rapid breeding and propagation of fraxinus americana tissue culture and application thereof. The medium employs an MS or a WPM medium as a minimal medium, and the medium is added with growth augmentors to a final concentration of: CPPU 0.05-0.5mg / L, NAA or IBA 0.1-0.5mg / L, and white granulated sugar 20-40g / L, and a pH of the medium is adjusted to 5.8-5.9. In the invention, plant-growth regulators of CPPU and NAA or IBA are added to the two phase solidified MS or WPM medium, so that the propagation effect on fraxinus americana embryo inducing aseptic seedling stems is substantial. The invention also provides application of the medium in rapid breeding and propagation of fraxinus americana tissue culture, so as to provide a feasible scheme for factory production of fraxinus americana in the future.

Description

technical field [0001] The invention relates to tree tissue culture, in particular to a medium for rapid propagation and proliferation of American white wax tissue culture and its application. Background technique [0002] American white wax (Fraxinus americana), also known as white ash, is a deciduous tree of Oleaceae, native to North America. American white wax is drought-resistant, cold-resistant, waterlogging-resistant, and saline-alkali-resistant. It is an excellent tree species for coastal wetland afforestation. It grows fast, has good greening effect, high ecological benefits, and has excellent material, hard texture, straight texture, fine and uniform structure, strong corrosion resistance, elasticity and luster, and easy processing. It is known as "Ash" ( Literature 1. Dong Bihui, Su Guoxing. Biological characteristics and economic uses of American ash. Jiangsu Forestry Science and Technology, 2003, 30, (1): 32-34). For a long time, white wax mainly reproduces by ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 叶景丰马冬菁陈罡范俊岗潘文利顾宇书高军魏忠平韩友志
Owner LIAONING FORESTRY SCI RES INST
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