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Method for simultaneously analyzing influenza A virus subtype and virulence thereof by utilizing agglutinin chip

A technology for influenza A virus and lectin, which is applied in the field of glycoprotein analysis, can solve the problem of inability to detect influenza A virus subtypes rapidly and simultaneously, and achieve the effect of improving sensitivity

Inactive Publication Date: 2012-09-05
NORTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for analyzing influenza A virus subtypes and their virulence using a lectin chip to solve the technical problem that conventional influenza virus detection methods cannot quickly and simultaneously detect influenza A virus subtypes and their virulence

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  • Method for simultaneously analyzing influenza A virus subtype and virulence thereof by utilizing agglutinin chip
  • Method for simultaneously analyzing influenza A virus subtype and virulence thereof by utilizing agglutinin chip
  • Method for simultaneously analyzing influenza A virus subtype and virulence thereof by utilizing agglutinin chip

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Embodiment Construction

[0053] A method for simultaneously analyzing influenza A virus subtypes and their virulence using a lectin chip, the method comprising the following steps:

[0054] 1) Prepare a lectin chip;

[0055] 1.1) Take the epoxy film base for subsequent use;

[0056] 1.2) Prepare the lectin into a sample solution with a concentration of 1 mg / ml;

[0057] 1.3) Add the prepared spotting solution to the 384-well plate; then use the crystal core 48 spotting system to spot the sample on the epoxy substrate;

[0058] 1.4) Incubate the spotted chip for 3 hours in an environment with a humidity of 60%;

[0059] 1.5) Vacuumize the incubated chip for 3 hours at 37°C to dry the chip and immobilize the lectin on the chip;

[0060] 1.6) Place the immobilized lectin chip in a desiccator for future use.

[0061] 2) Preparation of biological samples;

[0062] 2.1) Prepare the required high or low pathogenicity samples;

[0063] Highly pathogenic samples include H5N1 (DK / GD / 17 / 08), H5N1 (CK / GX / 4 / ...

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Abstract

The invention relates to a method for simultaneously analyzing influenza A virus subtype and virulence thereof by utilizing an agglutinin chip, and the method comprises the following steps: firstly, preparing the agglutinin chip and preparing a biological sample; and then, loading the biological sample to the agglutinin chip to detect the glycoprotein chain in the influenza A virus, thereby rapidly judging the subtype of the influenza A virus and the pathogenicity density of the influenza A virus. The method provided by the invention can be used for solving the technical problem that the conventional method for detecting influenza virus cannot be used for rapidly and simultaneously detecting the influenza A virus subtype and the virulence thereof; and the method provided by the invention has the advantages of rapidness, simplicity, convenience, high efficiency and the like.

Description

technical field [0001] The invention relates to a method for analyzing glycoproteins, in particular to a method for analyzing influenza A virus subtypes and their virulence using a lectin chip. Background technique [0002] Lectins are a class of glycoproteins with specific sugars that can promote cell aggregation. They can bind specific, non-covalent and reversible to sugars. According to the type of sugar bound, lectins in nature can be divided into several types, that is, they can specifically bind to a certain sugar molecule, which is called the sugar lectin. In this way, lectins can generally be divided into six categories: D-mannose Sugar or D-glucose lectins (such as concanavalin A); N-acetylglucosamine lectins (such as malt lectins); N-acetylgalactosamine lectins (such as soybean lectins); D-semi Lactose agglutinin (such as lectin of castor plant); L-fucose agglutinin (such as lectin of gorse); N-acetylneuraminic acid (sialic acid) lectin (such as wheat germ aggluti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/40G01N1/42G01N21/64
Inventor 李铮杜亚蓉孙宇祁会彩邓炜娜王秀荣
Owner NORTHWEST UNIV
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