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Gliocladium roseum chlamydospore and method for producing wettable powder thereof

A technology of Glioma pink and wettable powder, which is applied in the production field of Glioma pink chlamydospore and its wettable powder, can solve the problem of low recovery rate of spores, and meet the requirements of large-scale industrial production and low production cost , The effect of simplifying the production process

Inactive Publication Date: 2011-09-07
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, it overcomes the problem of low spore recovery rate in the existing preparation process of Glioma pink spore preparations, and provides a low-cost, simple-to-operate preparation method of wettable powder of Glioma pink spores

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Solid-state fermentation produces Glioma pink chlamydospores

[0022] Inoculate the Glioma rosea strain on a PDA plate and culture it at 24°C for 7 days to produce a large number of pink spores. Transfer to shake flasks for 3 days, and then transfer to 20 liter seed tanks for 3 days. Adjust the spore concentration to 10 with sterile water 6spores / ml. Use this spore suspension as liquid seed. After inoculating the prepared liquid seed spore suspension into the sterilized solid-state fermentation medium (30% wheat bran, 4% corn flour, and distilled water as the rest), in the first stage of fermentation, the temperature is 25°C and the plastic film is opened. The porosity is 2.5%, and the mycelium grows plentifully after being cultivated in the dark for 12 hours and then cultivated in the light for 4 days. In the second stage, the temperature is 20°C, the opening rate of the plastic film is 0.3%, and the light is cultivated for 4 days. The spores are cover...

Embodiment 2

[0023] Example 2 The preparation of Glioma pink chlamydospore wettable powder

[0024] After the solid-state fermentation is completed, the culture medium covered with chlamydospores of Glioma pinkis is put into a vacuum drying oven at 30°C for drying, and after drying for 12 hours, samples are taken to measure the moisture content. When the moisture content reaches 5%, the drying is complete. The dry spore-containing compost was pulverized by air flow at low temperature (5°C), and passed through a 325-mesh sieve to obtain fresh Gliocladium pink spore powder with a spore content of 6.24×10 8 per g, the live spore rate is greater than 98%. Prepare Glioma pink spore wettable powder according to the following prescription: Glioma pink spore powder 10%, diatomaceous earth 85%, nonylphenol polyoxyethylene ether formaldehyde condensate 2.5%, nonylphenol polyoxyethylene ether 2% , ascorbic acid 0.5%. Add diatomaceous earth, nonylphenol polyoxyethylene ether formaldehyde condensate...

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PUM

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Abstract

The invention provides Gliocladium roseum chlamydospore and a method for producing wettable powder thereof, belonging to the technical field of biological pesticides. In the invention, the method of solid state fermentation is adopted, the fermentation temperature and oxygen supply are controlled to produce the Gliocladium roseum chlamydospore; and compost with the chlamydospore is dried and smashed to prepare the wettable power by adding a corresponding addition agent. The invention aims to provide a method for producing the Gliocladium roseum chlamydospore in a large scale at low cost to solve the problem that Conidium has short survival time and shelf life and ensures biological activity and preventive effects of the Gliocladium roseum chlamydospore in production and application. Meanwhile, the method solves the problem that spore recovery rate is low in the preparation process of the Gliocladium roseum chlamydospore and provides a method for preparing the wettable powder of the Gliocladium roseum chlamydospore, which features low cost and simpleness in operation. The Gliocladium roseum chlamydospore and the wettable powder thereof can be applied to treating multiple vegetable diseases, including gray mold, seed-borne diseases and soil-borne diseases.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular the invention relates to a production method of chlamydospores of Glioma pink and its wettable powder. Background technique [0002] my country is a large agricultural country, and the area affected by crop diseases, insect pests and weeds is about 200 million hectares (times) every year, and about 250,000 tons of pesticides (active ingredients) need to be produced and used every year. The extensive use of chemical pesticides has made my country one of the countries with the most serious pesticide pollution in the world. The detection rate of pesticides in food is as high as 90%, and the farmland environment and food safety are worrying. With the development of harmless agriculture and the change of social requirements, the trend of using living organisms as pesticides is getting higher and higher in recent years. Developed countries in the world are actively developing bi...

Claims

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Application Information

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IPC IPC(8): C12N3/00A01N63/04A01P3/00C12R1/645
Inventor 张媛媛刘均洪吴汝林王繁业宿烽
Owner QINGDAO UNIV OF SCI & TECH
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