Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of core-shell magnetic conductive polymer microspheres and application thereof

A conductive polymer and polymer technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of unfriendly environment and increased production cost

Inactive Publication Date: 2012-09-19
QILU UNIV OF TECH
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The heterogeneous polymerization method can obtain high-quality core-shell magnetic composite microspheres, but cross-linking agents and / or surfactants need to be added during the preparation process; this not only increases the production cost, but is also environmentally unfriendly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of core-shell magnetic conductive polymer microspheres and application thereof
  • Preparation method of core-shell magnetic conductive polymer microspheres and application thereof
  • Preparation method of core-shell magnetic conductive polymer microspheres and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Core-shell Fe 3 O 4 / PANI composite microsphere preparation

[0056] Add 0.1g Fe into a 100mL three-necked flask 3 O 4 , 40mL 0.1mol / L hydrochloric acid, ultrasonic dispersion at room temperature for 5 minutes, sealed and placed in a 5 ℃ constant temperature box for 12 hours, magnetic separation to remove the aqueous hydrochloric acid solution, retaining the magnetic core; then add 15mL absolute ethanol, 0.1 g Re-distilled aniline, ultrasonically disperse for 5 minutes, and place it in a thermostat at 5°C for 12 hours.

[0057] Place the flask in an ultrasonic cleaner, and control the water temperature of the ultrasonic cleaner to 5-10°C. Dissolve 0.25mL of 12mol / L hydrochloric acid in 45mL of distilled water, then add 0.28g of ammonium persulfate to dissolve, slowly add the oxidant solution dropwise to control the dropping time to 0.5h; the reaction is maintained and stirred with the assistance of ultrasound for 3h.

[0058] Solid-liquid magnetic separation, water...

Embodiment 2

[0059] Example 2: Core-shell Fe 3 O 4 / PPy composite microsphere preparation

[0060] Replace the aniline in Example 1 with pyrrole, and use 0.40g FeCl for 0.28g ammonium persulfate 3 ·6H 2 O instead, other operations remain unchanged, you can get Fe 3 O 4 / PPy sample.

Embodiment 3

[0061] Example 3: Using Fe 3 O 4 / PANI composite microspheres to separate and purify E.coli DH5α plasmid DNA

[0062] Take 10mg de-doped Fe 3 O 4 / PANI magnetic ball is placed in a 1.5mL sterile centrifuge tube, sonicated with 1mL 70% (v / v) ethanol solution, sterilized twice, then transferred to another sterile centrifuge tube, and then added 40μL E.coli DH5α The crude plasmid DNA extract and 1mL Tris-HCl (10mmol / L, pH 7.8) buffer, shake well, and place it at room temperature for 20 minutes until the DNA is adsorbed on the surface of the magnetic ball. Collect the magnetic ball under a magnetic field and discard the supernatant. Wash the magnetic balls twice with 1 mL of 70% ethanol solution, magnetically separate, discard the supernatant, and place the magnetic balls adsorbing DNA at room temperature to volatilize the ethanol.

[0063] Add 0.4mL of 2mol / L NaCl solution to the centrifuge tube containing the magnetic ball, shake well, place at room temperature for 30 minutes until D...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of core-shell magnetic conductive polymer microspheres. Ferrite (Fe3O4, gamma-Fe2O3, MFe2O4; M = Fe, Co, Ni, Mn and the like) nano or micro spherical or near-spherical particles are used as cores; firstly, surface hydroxyl protonation is performed on the magnetic cores to fully combine the magnetic cores before compositing with monomer molecules by theelectrostatic interaction and hydrogen-bond action between the protonated hydroxy and monomer aniline, pyrrole or derivatives thereof; and in the oxidation polymerization process, the monomer molecules are controlled to form cores and grow on the surfaces of the magnetic cores, thereby preparing the core-shell magnetic conductive polymer microspheres with high magnetic responsibility and good dispersity. By the characteristics that the amido-containing magnetic polymer microspheres are positively charged in a high-pH solution while the DNA molecules in a tissue or cell lysis solution are negatively charged, the prepared magnetic composite microspheres are applied to DNA separation and purification assisted by an external magnetic field.

Description

Technical field [0001] The invention relates to a method for preparing core-shell magnetic conductive polymer microspheres and its application in DNA separation and purification, and belongs to the technical field of magnetic composite materials and biological separation materials preparation. Background technique [0002] The separation and purification of DNA has a very important position in the fields of genetic engineering, biology, medicine and genetics. DNA separation and purification technologies can be generally divided into two categories: no external magnetic field assistance and external magnetic field assistance, namely non-magnetic separation and magnetic separation. There are many methods of non-magnetic separation, which are described in many documents and patents (N. Ramashupuramanyan Chinese Patent: DNA Purification Method and Purified DNA. CN 1390229A, January 8, 2003), Such as: phenol-chloroform-isoamyl alcohol method, cesium chloride-ethidium bromide density ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/02C08G73/06C08K9/02C08K3/22C12N15/10
Inventor 盖利刚马万勇姜海辉韩小云
Owner QILU UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com