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Fluorescent latex particles and preparation method thereof

A latex particle and fluorescence technology, applied in the field of fluorescent latex particles and their preparation, can solve the problems of uneven distribution of fluorescent molecules, poor monodispersity, and easy leakage, and achieves clean and clean surface, mild process conditions, and good monodispersity. Effect

Active Publication Date: 2011-09-07
SINOCARE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is simple to prepare fluorescent latex particles, but the distribution of fluorescent molecules in the particles is not uniform, the combination is not firm, and it is easy to leak out, especially when used in some non-polar solutions, organic dyes are very easy to be leached
[0007] The polymerization method of fluorescent latex particles generally adopts conventional emulsion polymerization or dispersed emulsion polymerization, but the formula and process of these preparation methods are relatively complicated, and the prepared particles have uneven particle size, poor monodispersity, unclean particle surface, and difficult to control particle size. , which is not conducive to the application in biological detection

Method used

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  • Fluorescent latex particles and preparation method thereof
  • Fluorescent latex particles and preparation method thereof
  • Fluorescent latex particles and preparation method thereof

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preparation example Construction

[0032] The invention provides a method for preparing fluorescent latex particles, comprising a) mixing acrylate monomers, fluorescent dyes and ultrapure water at a weight ratio of 1-15:0.004-0.4:100, stirring, heating, and Soap-free emulsion polymerization occurs under the action of an initiator to obtain a first reaction product;

[0033] b) filtering and centrifuging the reaction product obtained in step a) to remove the supernatant, and washing the residue after removing the supernatant to obtain fluorescent latex particles.

[0034]What the present invention uses is soap-free emulsion polymerization, and so-called soap-free emulsion polymerization refers to the emulsion polymerization process that does not add emulsifier at all or only adds trace emulsifier (its concentration is less than the critical micelle concentration CMC) in the reaction process, also known as Emulsifier-free emulsion polymerization, the present invention does not add any emulsifier, when preparing f...

Embodiment 1

[0048] Example 1: Carboxyl fluorescent latex particles with a size of about 250nm

[0049] Add 570ml of ultra-pure water, 10ml of MMA, 0.02g of Rhodamine B, and 18mg of sodium styrene sulfonate into the reaction kettle, mix and heat together with stirring at a stirring speed of 150rpm, and pass nitrogen gas for 30min to 60min to exhaust the dissolved oxygen in the reaction system as much as possible. When the temperature was raised to 70°C, 20ml of potassium persulfate was added to start timing. After 90 minutes of reaction, MAA and MMA were added. The ratio between them was 1:1, and the reaction stopped after 2 hours. After cooling, the particles were filtered, and then the particles were centrifuged in a 50ml centrifuge tube, washed 3 times to obtain fluorescent latex particles.

Embodiment 2

[0050] Example 2: Carboxyl fluorescent latex particles with a size of about 300nm

[0051] Add 565ml of ultra-pure water, 15ml of MMA, 0.02g of rhodamine 6G, and 18mg of sodium styrene sulfonate into the reaction kettle, mix and stir together and heat at a stirring speed of 180rpm, and pass nitrogen gas for 30min to 60min to exhaust the dissolved oxygen in the reaction system as much as possible. do. When the temperature was raised to 70° C., 20 ml of potassium persulfate was added to start timing. After 120 minutes of reaction, MAA and MMA were added, and the ratio between them was 1: 1. The reaction stopped after continuing for 2 hours. After cooling, the particles were filtered, and then the particles were centrifuged in a 50ml centrifuge tube, washed 3 times to obtain fluorescent latex particles.

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Abstract

The invention provides a method for preparing fluorescent latex particles, which comprises the following steps of: a) mixing an acrylic ester monomer, a fluorescent dye and ultrapure water in a weight ratio of (1-15):(0.004-0.4):100, stirring, heating, and performing soap-free emulsion polymerization under the action of an initiator to obtain a first reaction product; and b) filtering and centrifuging the reaction product obtained in the step a), removing supernatant, and washing residues left after the supernatant is removed to obtain the fluorescent latex particles. Process conditions in the preparation method are mild, and the prepared fluorescent latex particles have a uniform and controllable particle size and high monodispersity.

Description

technical field [0001] The invention relates to the field of polymer synthesis, and more particularly to a fluorescent latex particle and a preparation method thereof. Background technique [0002] With the continuous development of medical diagnostic technology, the detection limit of target analytes is constantly decreasing, so it is necessary to continuously develop antigen or antibody labeling technology. At present, the labeling technology mainly uses radioactive isotopes, colloidal gold, fluorescent molecules, enzymes, latex particles, etc. They all have their own advantages, but for complex clinical samples, the development of detection technologies such as high-throughput, microquantification, automation, and sensitivity cannot be realized. A polymer material combining fluorescent dye molecules with latex particles provides a powerful means for realizing this detection technology. Because it not only has the high sensitivity of fluorescent molecules, but also has t...

Claims

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Application Information

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IPC IPC(8): C08F220/14C08F220/18C08F220/06C08F212/14C08F2/22C08F2/44C09K11/06
Inventor 聂海龙
Owner SINOCARE
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