Silkworm cocoon health-care preparation and production method thereof
A technology for silkworm cocoons and preparations, which is applied in the field of health care preparations and can solve the problems of large adverse reactions of immediate-release preparations
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preparation Embodiment 1
[0064] Preparation of enzymatically hydrolyzed cocoon fibroin 1
[0065] The solid cocoon silk fibroin taken from the silk reeling factory, the solid silk fibroin is treated with 0.5% Na at 90-100°C before use3 CO 3 The solution is degummed to cocoon silk fibroin for 20-30 minutes at a solid-to-liquid weight ratio of 1:10, and filtered to obtain silk fibroin solids, which are dissolved in CaCl with a weight content of 20-40% at 40-70°C 2 In aqueous solution (according to 1 weight of silk fibroin: 10 weights of CaCl2 aqueous solution), the time is 1 hour, and then every kilogram of CaCl 2 Add 0.5 g of papain to the aqueous solution for enzymatic hydrolysis at room temperature for 2 hours, and use a MWCO 500 ultrafiltration membrane to filter out small molecular substances such as pigments and peculiar smells. The enzymatic silk fibroin obtained by drying is preserved for future use.
preparation Embodiment 2
[0067] The solid cocoon silk fibroin taken from the silk reeling factory is treated with 1% Na at 70-85°C before use. 3 CO 3 The solution is degummed to cocoon silk fibroin for 20-30 minutes at a solid-to-liquid weight ratio of 1:15, and filtered to obtain silk fibroin solids, which are dissolved in CaCl with a weight content of 20-40% at 40-70°C 2 In aqueous solution (according to 1 part by weight of silk fibroin: 10 parts by weight of CaCl2 aqueous solution), the time is 1.5 hours, and then every kilogram of CaCl 2 Add 1 gram of papain to the aqueous solution for enzymatic hydrolysis at room temperature for 1 hour, use MWCO 500 ultrafiltration membrane to filter out small molecular substances such as pigment and peculiar smell, and dry to obtain enzymatic silk fibroin for future use.
preparation Embodiment 3
[0069] Soak the cocoon and pupal lining in hot water at 70-100°C with a solid-to-liquid weight ratio of 1:10-15 for 1-2 hours, then boil for 0.5-2 hours, filter to remove residue, and prepare the liquid for later use, or concentrate the liquid to obtain silk fibroin cream Save the body for later use.
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