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Use of Bordetella pertussis CpG-ODNs (CpG oligodeoxynucleotides) as medicine for preventing and treating asthma

A technology of whooping cough and cpg-odns, which can be applied in drug combinations, gene therapy, and pharmaceutical formulations, and can solve problems such as ineffectiveness of human allergic asthma

Inactive Publication Date: 2011-08-31
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that the artificially synthesized CpG-ODNs sequence can inhibit allergic asthma in animal models, but it is almost ineffective against human allergic asthma, which is related to the different recognition sites of CpG-ODNs by TLR-9, based on the whole-cell Bacillus pertussis species-free Specificity, we analyzed that its CpG-ODNs sequence is different from artificially synthesized CpG-ODNs, and it has anti-allergic asthma activity on humans and animals

Method used

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  • Use of Bordetella pertussis CpG-ODNs (CpG oligodeoxynucleotides) as medicine for preventing and treating asthma
  • Use of Bordetella pertussis CpG-ODNs (CpG oligodeoxynucleotides) as medicine for preventing and treating asthma
  • Use of Bordetella pertussis CpG-ODNs (CpG oligodeoxynucleotides) as medicine for preventing and treating asthma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] 1. Preparation of B. pertussis CpG-ODNs

[0014] This experiment was based on the genome sequence of the WPI international standard strain provided by Genebank. Select the Genome option in Genebank, search and download the full genome sequence of Bordetella pertussis, and import the full sequence file into the Vector NTI Advance11 software; consult the literature to obtain three CpG-ODNs with anti-allergic asthma activity (ODN-1018, ODN-1026, ODN-3234); use the vector NTI Advance software to compare (Blast) known effect sequences ODN-1018, ODN-1026, ODN-3234 with the B. pertussis genome, and obtain 9 sequences with the highest homology, 9 The sequence is as follows:

[0015] name

Sequences

Seq A

5'-TCCAAGACGTTCATGGCGAT-3' (SEQ ID NO: 1)

Seq B

5'-TGCATGACGATCCTGCCGAT-3' (SEQ ID NO: 2)

Seq C

5'-ACCGCGACGTTATAGGCGTT-3' (SEQ ID NO: 3)

Seq D

5'-ATGACGGTAACGACCGGATG-3' (SEQ ID NO: 4)

Seq E

5'-AAGAAGCTGACGTT ...

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Abstract

The invention provides a novel medicine for preventing and treating asthma, which is Bordetella pertussis CpG-ODNs (CpG oligodeoxynucleotides) with a sequence as shown by SEQIDNO.1. The Bordetella pertussis CpG-ODNs (seqA) can inhibit inflammatory response of the asthma and prevent or control asthmatic attack. With generation of signal transduction by using a TLR-9 (Toll-like Receptor) signal molecule and Th1 / Th2 (T helper 1 / T helper 2) unbalance as theoretical basis, effects of the seqA on adjustments of asthma related cell factors and inhibition of airway inflammation are found in the invention. The invention breaks through a traditional asthma treatment method of using inflammation prevention and relaxation of an airway smooth muscle as a target point and establishes a new way with asthma control as main, thereby building up a new way for controlling asthmatic attack and preventing the asthma.

Description

technical field [0001] The patent of the present invention discloses a nucleotide sequence of Bacillus pertussis (named seq A) and its application. The seq A can prevent and treat asthma through the TOLL signal molecule. Background technique [0002] Unmethylated oligonucleotide single-stranded (CpG-ODNs) is a DNA sequence mainly present in bacteria, which can bind to the endosomal TOLL-like receptor (TLR) 9 of immune cells (dendritic cells, etc.) Forms dimers and inhibits allergic asthma by regulating Th1 / Th2 balance. Bacillus pertussis is a type of pathogenic microorganism. Routine vaccination of standardized pertussis vaccine can significantly reduce the incidence of pertussis disease. Whole-cell pertussis vaccine selectively enhances the Th1 response of humans and animals, which is related to the CpG-ODNs in the bacteria. It can significantly reduce the airway inflammation and cytokine secretion in mouse asthma models, maintain the balance of Th1 / Th2, and relieve asthma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105A61K48/00A61P11/06
Inventor 孙云王德俊张宝袁迟深
Owner YANGZHOU UNIV
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