Ultra high speed and high sensitivity DNA base sequence analysis system and analysis method for same

Abstract

The present system relates to a system architecture that uses a single electron transistor (SET) to analyze base sequences of deoxyribonucleic acid (DNA) at ultra high speed in real time. DNA represents the entire body of genetic information and consists of nucleotide units. There are a total of four types of nucleotides, and each nucleotide consists of an identical pentose (deoxyribose), and four types of bases that differ in their phosphate groups (Adenine: A, Guanine: G, Cytosine: C, Thymine: T). A and G are purines having a bicyclic structure while C and T are pyrimidines having a monocyclic structure. Each has a different atomic arrangement, which signifies a different charge distribution from one another. Therefore, a system comprising a single electron transistor that is very sensitive to charges, a probe of a very small size that reacts to one nucleotide very effectively, and an extended gate that connects the SET with the probe, can be used to analyze DNA base sequences at ultra high speed in real time.

Description

technical field [0001] The present invention relates to a method and a system structure for analyzing the base sequence of deoxyribonucleic acid. More specifically, it relates to an analysis method and system configuration capable of analyzing a large amount of deoxyribonucleic acid base sequences in real time at ultrahigh speed compared with the analysis methods currently used. Background technique [0002] Deoxyribonucleic acid is the totality of genetic information, composed of nucleotide units. Proteins are synthesized according to the sequence of nucleotides recorded in deoxyribonucleic acid, and when a base sequence differs from the original base sequence, a different protein will be synthesized, causing serious problems. Therefore, from the viewpoint of disease prevention and treatment, it is very important and necessary to check whether the deoxyribonucleic acid has the correct nucleotide sequence. [0003] There are four types of nucleotides, each of which consist...

AI Technical Summary

Problems solved by technology

However, these methods have disadvantages in that the number of bases analyzed per unit time is small, and it takes a long time for preparations such as replacing radioactive isotopes or dyes

In addition, not only the cost is high, but also environmental pollutants such as radioactive waste are discharged after the analysis is completed.

[0005] In addition, the length of DNA that can be analyzed is limited, and it is difficult to analyze several DNAs at the same time

[0006] At present, although various methods for analyzing the base sequence of deoxyribonucleic acid have been developed, there are problems in that the number of bases analyzed per unit time is small, and in the preparation work such as replacing radioactive isotopes or dyes, etc. takes a long time

In addition, not only the cost is high, but also environmental pollutants such as radioactive waste are discharged after the analysis is completed.

[0007] In addition, the length of DNA that can be analyzed is limited, and it is difficult to analyze several DNAs at the same time

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