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Culture medium for vibrio BS02 and preparation method thereof

A kind of culture medium, technology of Vibrio, applied in the culture medium of Vibrio BS02 and its preparation field

Inactive Publication Date: 2011-08-17
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the use of algicidal bacteria to control red tide is still in its infancy, and the red tide disaster has the characteristics of complexity and diversity. It is necessary to further optimize its fermentation conditions on the basis of understanding the algicidal mechanism of algicidal bacteria, so as to produce efficient algicide

Method used

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  • Culture medium for vibrio BS02 and preparation method thereof
  • Culture medium for vibrio BS02 and preparation method thereof
  • Culture medium for vibrio BS02 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Screen the appropriate basal medium, select the appropriate medium components, use the Plackett-Burman (PB) design method to screen the significant factors, find the optimal area of ​​the significant factors through the steepest climbing test, and then use the Box-Behnken design method to establish regression Equation, and finally find the extreme value of the equation to get the optimal combination. The experimental design and data analysis in the present invention adopt Minitab 13.0 data processing software.

[0058] 1) The relationship between biomass and algicidal effect

[0059] The activated BS02 strain was transferred to an Erlenmeyer flask containing 100mL 2216E according to the inoculum amount of 1%, and 3 parallels were used. Take samples every 4 hours to measure OD 600 , centrifuged to remove the cells, and stored in a refrigerator at 4°C to determine the algicidal effect.

[0060] The results showed that the algicidal effect gradually increased with the i...

Embodiment 2

[0067] According to the previous experiments, the culture medium of Vibrio BS02 was optimized based on the response surface analysis method:

[0068] 1) Plackett-Burman experimental design

[0069] The Plackett-Burman design method is a partial factorial experimental design method based on the principle of balanced incomplete blocks. It is a two-level experimental design method that tries to achieve the main effect of the factor with the least number of trials. Obtaining an estimate as accurate as possible is suitable for quickly and effectively screening out the most important factors from a large number of investigation factors for further research. For N experiments, at most (N-1) factors can be studied, but the actual factors should be less than N-1, and at least one imaginary variable should be used to estimate the error. Two levels are taken for each factor: the low level is the original culture condition, and the high level is about 1.25 times of the low level.

[007...

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Abstract

The invention relates to a culture medium for vibrio BS02 and a preparation method thereof, relating to a microbial culture medium and providing a culture medium which is suitable for culture of the vibrio BS02 and can obviously increase cell live weight of the vibrio BS02 and yield of algae-killing active substances. The preparation method comprises the steps of: adding tryptone, yeast powder, glucose, NaCl, MgSO4.7H2O, KCl, K2HPO4.3H2O, CaCl2.2H2O and Fe2(SO4)3 into distilled water, enabling a constant volume to be 1L, and sterilizing to obtain the culture medium of the vibrio BS02. A composite culture medium suitable to high-dense culture of the vibrio BS02 is developed by taking an LB (Luria-Bertani) culture medium as a basic culture medium, investigating the influence of the components of the culture medium and the addition amount thereof to shaking culture of the vibrio BS02, and optimizing the culture medium by utilizing a Plackett-Burman (PB) design method and a response surface optimized design method.

Description

technical field [0001] The invention relates to a microorganism culture medium, in particular to a culture medium of Vibrio BS02 and a preparation method thereof. Background technique [0002] With the rapid development of modern industrialization and the increase of population, a large amount of industrial and agricultural wastewater and domestic sewage are directly discharged into the ocean without any treatment, causing the eutrophication of marine water to intensify, and red tides frequently occur (1, Hallegraeff GM. A review of harmful algal blooms and their apparent global increase [J]. Phycologia. 1993, 32: 79-99). Harmful red tide has become a global marine disaster, seriously restricting the development of coastal economy, destroying the marine ecological environment and threatening human health. Therefore, how to effectively prevent and control red tide has become an urgent problem at home and abroad. [0003] In the marine ecosystem, microorganisms play an import...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/63
Inventor 郑天凌安新丽傅丽君田蕴
Owner XIAMEN UNIV
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