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Halophilic bacterial agent and preparation method thereof as well as biological treatment system fixed with bacterial agent and application thereof

A technology of biological treatment and halophilic bacteria, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of staying in theory and few industrial applications, and achieve simple operation, realization of waste water resources and utilization, and process flow short effect

Active Publication Date: 2012-01-04
SHANDONG WEIFANG RAINBOW CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The premise of using biochemical methods to treat phenol-containing wastewater is to screen out microorganisms that have a good degradation effect on phenolic compounds. Because the phenol-containing wastewater discharged from some industries contains high salt concentrations in addition to phenolic compounds, such as the production of nitric acid in the chemical industry In this high-salt environment, the activity of microorganisms will be inhibited as long as the salt concentration is >3000mg / L. The treatment of phenol wastewater has important significance and broad application prospects, and this kind of research basically stays in theory, and there are few industrial applications

Method used

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  • Halophilic bacterial agent and preparation method thereof as well as biological treatment system fixed with bacterial agent and application thereof
  • Halophilic bacterial agent and preparation method thereof as well as biological treatment system fixed with bacterial agent and application thereof
  • Halophilic bacterial agent and preparation method thereof as well as biological treatment system fixed with bacterial agent and application thereof

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Embodiment 1

[0073] The strains screened by the present invention are mixed according to the ratio to obtain the halophilic bacterial agent required for degrading phenol-containing wastewater. The bacterial agent consists of: 7 parts of CGMCCNO.4327, 4 parts of CGMCCNO.4328, 9 parts of CGMCCNO.4329, CGMCCNO.4330 8 copies.

[0074]The preparation method of the halophilic bacterial agent is as follows: respectively inoculate 4 kinds of bacteria into the culture medium, the inoculum amount is 10wt%, and the temperature is 15-35°C, pH 8.5-9.5, dissolved oxygen 2-4mg / L The strains are expanded and cultivated under certain conditions, and the cultivation time is 72-96h. The medium used is glucose 9-11g / L, urea 1.0-1.2g / L, potassium dihydrogen phosphate 1.29-1.34g / L, and sodium chloride 15wt%. After culturing, each culture medium is centrifuged to obtain wet bacteria, and the wet bacteria are mixed according to the above ratio to obtain the halophilic bacteria agent.

Embodiment 2

[0076] The strains screened by the present invention are mixed according to the ratio to obtain the halophilic bacterial agent required for degrading phenol-containing wastewater. The bacterial agent consists of: 4 parts of CGMCCNO.4327, 8 parts of CGMCCNO.4328, 3 parts of CGMCCNO.4329, CGMCCNO.4330 10 copies.

[0077] The preparation method of the halophilic bacteria agent is as follows: inoculate 4 kinds of bacteria into the culture medium respectively, the inoculum amount is 10wt%, under the conditions of temperature 15-35°C, pH 8.5-9.5, dissolved oxygen 2-4mg / L The halophilic bacteria strains were expanded and cultivated for 72-96 hours. The medium used is glucose 9-11g / L, urea 1.0-1.2g / L, potassium dihydrogen phosphate 1.29-1.34g / L, sodium chloride 4wt%. After culturing, each culture medium is centrifuged to obtain wet bacteria, and the wet bacteria are mixed according to the above ratio to obtain the halophilic bacteria agent.

Embodiment 3

[0079] The strains screened by the present invention are mixed according to the ratio to obtain the halophilic bacterial agent required for degrading phenol-containing wastewater. The bacterial agent consists of: 10 parts of CGMCCNO.4327, 10 parts of CGMCCNO.4328, 5 parts of CGMCCNO.4329, CGMCCNO.4330 4 copies.

[0080] The preparation method of the halophilic bacterial agent is as follows: respectively inoculate 4 kinds of bacteria into the culture medium, the inoculum amount is 10wt%, and the temperature is 15-35°C, pH 8.5-9.5, dissolved oxygen 2-4mg / L The halophilic bacteria strains were expanded and cultivated for 72-96 hours under certain conditions. The medium used is glucose 9-11g / L, urea 1.0-1.2g / L, potassium dihydrogen phosphate 1.29-1.34g / L, sodium chloride 24wt%. After culturing, each culture medium is centrifuged to obtain wet bacteria, and the wet bacteria are mixed according to the above ratio to obtain the halophilic bacteria agent.

[0081] The application ...

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Abstract

The invention discloses a halophilic bacterial agent and a preparation method thereof as well as a biological treatment system fixed with the bacterial agent and application thereof. In the invention, strains are screened from sewage, sludge and soil to be prepared into a bacterial agent, wherein the bacterial agent comprises four strains including Providenciasp. CJ-4, Oceanobcillussp. CJ-10, Brevibacterium epidermidis CJ-12 and Halomonassp. CJ-13. The invention also provides the biological treatment system fixed with the bacterial agent and a method for treating waste water by using the same. The bacterial agent disclosed by the invention has stronger degradation capability on phenolic wastewater, can adapt to high-salt environments and has very good degradation effects under an environment with the salt content of 2-25% and the phenol concentration of 2500 mg / l; and compared with the traditional active sludge method, the bacterial agent has obvious advantages in the performance of degrading phenolic compounds, and the degradation effect on the phenolic compounds in water can reach more than 99.9%.

Description

technical field [0001] The invention relates to a halophilic bacterium and its application in wastewater treatment, in particular to a halophilic bacterium capable of efficiently degrading phenol in wastewater and its application in the treatment of high-salt and high-phenol wastewater, belonging to microorganisms and wastewater treatment technology field. Background technique [0002] Phenolic compounds have a special aromatic smell, are weakly acidic, and are easily oxidized in the environment. Due to the wide range of uses of phenol, the work of preventing its pollution is also very difficult. Phenolic wastewater is one of the most harmful and widely polluting industrial wastewater in the world today, and is an important source of water pollution in the environment. Phenol-containing wastewater mainly comes from coking plants (especially low-temperature soil coking), gas plants, petrochemical plants, insulation material plants and other industrial sectors, as well as pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F101/34C12N1/20C01D3/06C12N1/00C12R1/01C02F9/14
Inventor 袁从伟孙国庆李志清王海燕凌晓光
Owner SHANDONG WEIFANG RAINBOW CHEM
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