Three-step method for producing 'dragon head phoenix tail' high-quality officinal dendrobium stem material
A technology of Dendrobium candidum, three-step method, which is applied in the field of "three-step method" to produce high-quality Dendrobium candidum material of "dragon head and phoenix tail". The seedling time is short, easy to master, and the effect of simple steps
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Embodiment 1
[0037] (1) Embryo culture directly into seedlings: the mature fruit pods of Dendrobium candidum (Dendrobium candidum seedlings are purchased from the Orchid Association of Yueqing City, Zhejiang Province, and are mature fruit pods obtained by cultivating for 5 months after artificial cross-pollination) through volume percentage 75% alcohol Disinfect the surface for 7 minutes, burn the soaked fruit pods with an alcohol lamp flame for 2-3 seconds, cut open the fruit pods with a scalpel, collect the seed embryos in a sterile serum bottle, add 5ml of sterile water to dilute and suspend, Take 1ml of the suspension, spread it evenly on the surface of sterile LCY medium, cultivate it under dark light for 10 days, and after 10 days, the seed embryos will expand, and then give 1000Lux light during the day (12 hours). After 30 days, the seed embryos germinate into small green buds, transplant the green buds to the LVB medium, transfer the rooted seedlings to the LPY medium after 60 days,...
Embodiment 2
[0046] (1) Embryo culture directly into seedlings: the mature fruit pods of Dendrobium officinale were sterilized with 75% alcohol by volume for 7 minutes, and the soaked fruit pods were burned with an alcohol lamp flame for 2 to 3 seconds, and the scalpel blades were cut open. Collect the seed embryos in a sterile serum bottle, add 5ml of sterile water to dilute and suspend, draw 1ml of the suspension, spread it evenly on the surface of sterile LCY medium, and cultivate it under dark light for 20 days. During the day (12 hours) 1500Lux light. After 45 days, the seed embryos germinate into small green buds, transplant the green buds to the LVB medium, transfer the rooted seedlings to the LPY medium after 70 days, and after 80 days, each cluster of seedlings grows an average of 6 root systems, with an average seedling height of 10cm , move the test-tube seedlings into the greenhouse for hardening for 10 days, control the light at 2000-3000Lux, and the temperature at 23-25°C.
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Embodiment 3
[0055] (1) Embryo culture directly into seedlings: the mature fruit pods of Dendrobium officinale were sterilized with 75% alcohol by volume for 7 minutes, and the soaked fruit pods were burned with an alcohol lamp flame for 2 to 3 seconds, and the scalpel blades were cut open. Collect the seed embryos in a sterile serum bottle, add 5ml of sterile water to dilute and suspend, draw 1ml of the suspension, spread it evenly on the surface of sterile LCY medium, and cultivate it under dark light for 15 days. 1500Lux light. After 40 days, the seed embryos germinated into small green buds, and the green buds were transplanted to the LVB medium. After 65 days, the rooted seedlings were transferred to the LPY medium. After 70 days, each cluster of seedlings grew an average of 5.5 roots, and the average height of the seedlings was 9.6. cm, used as transplanted seedlings in the greenhouse.
[0056] The LCY medium is adding coconut water and yeast extract to LV-3 medium, wherein every li...
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