Expanded bed chromatographic separation device for biochemical separation technology

Abstract

The invention discloses an expanded bed chromatographic separation device for a biochemical separation technology, belonging to the biochemical field. A liquid distributor consisting of a feeding pipe, a radial spraying hole and a radial guiding disk is arranged under an expanded bed porous screen / sieve plate, and the bottom end of the expanded bed is provided with a material discharging hole. Through changing a circulation area of fluid flowing along a radial direction of the guiding disk, the liquid distribution pressure change caused by variable mass flowing and a runner changing in a fan-shaped geometrical shape is overcome / offset, the problem of uniform distribution of liquid supply pressure of feed liquid in the radius direction under low bed pressure drop in an industrial amplification production process of the expanded bed is solved, the fluid passing the bed is ensured to be in a flat pushing flow state, the use effect and the use efficiency of the expanded bed are greatly improved, and the adsorption capacity and the desorption resolution ratio of the expanded bed are improved. The invention ensures that the expanded bed technology can be applied to purification and separation of all protide substances produced by microorganisms and animal cells and solid particles or materials of sediments are directly used in the chromatographic separation.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a chromatographic separation device used for extraction / separation / purification in industrial scale production. Background technique [0002] Chromatographic separation is one of the most commonly used methods in biochemical separation processes. [0003] The traditional chromatographic separation column (referred to as chromatographic column) is in the form of a fixed bed, that is, the adsorption resin is pressed and fixed in the chromatographic column with upper and lower porous sieve plates, so that it cannot flow, commonly known as "fixed bed", and its specific structure can be found in the appendix Figure 1a shown. [0004] The characteristics of the traditional fixed bed are that due to the small porosity of the bed, the resistance of the fluid passing through the bed is relatively large, the fluid flows through the bed in a "plug flow", and the fluid velo...

AI Technical Summary

Problems solved by technology

[0027] The technical problem to be solved by this invention is to provide a kind of expanded bed chromatographic separation device for biochemical separation technology, which can better control the liquid entering the expanded bed by setting a liquid distributor under the expanded bed porous screen / sieve plate. The raw material samples are uniformly distributed, which solves the problem of uniform distribution of the feed liquid pressure along the radial direction under the low bed pressure drop in the industrial scale-up production process of the expanded bed, and better ensures that the fluid passing through the expanded bed layer is present. In the plug flow state, it can also automatically clean the porous screen / sieve plate and support plate, greatly improving the use effect and efficiency of the expanded bed, and improving the adsorption capacity and desorption resolution of the expanded bed

Images