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Artificial kidney precursor and process for production thereof

An artificial kidney and precursor technology, which is applied to artificial cell constructs, biochemical equipment and methods, and devices for human tubular structures, etc., can solve the problems of high skills, difficulty in obtaining functional artificial kidney precursors, and complicated procedures. , to the effect of avoiding the need for cultivation operations

Inactive Publication Date: 2011-06-22
OTSUKA PHARM FAB INC +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is a problem in this method that the procedure is complicated because it requires collecting amniotic fluid from a pregnant mammal just before transplanting the artificial kidney precursor to the patient, and confirming the biological safety of the precursor
In addition, the mesenchymal stem cells must be injected into the embryo at a suitable site where a kidney will form in the future, an operation that requires great skill
Also, because MSCs must be infused into embryos while the host's immune system is in a state of immune tolerance, there is low flexibility in the overall treatment schedule
In this method, it is difficult to obtain a functional artificial kidney precursor unless whole-embryo culture is performed, which creates a risk of contamination due to culture and requires complicated purification procedures after culture

Method used

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Examples

Experimental program
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Embodiment

[0059] On the 14th day of pregnancy, the metanephros was isolated from the rat embryo, and then cryopreserved in ET-Kyoto+10% DMSO+20% FCS. The diameter is about 2mm.

[0060] After cryopreservation for 2 days, the frozen metanephros was thawed. The rat mesenchymal stem cells were encapsulated in Matrigel, and then 1×10 4 One cell / metonephrine is injected into the metanephrosis. After that, metanephrosis was transplanted into the omentum of syngeneic rats.

[0061] Seven days after transplantation, the grown metanephros were isolated from the rats that received metanephros transplantation. The number of animals receiving metanephrosis was 2, and 3 and 6 were grown from the 7 metanephrosis. The metanephrosis grows to a diameter of 6-8mm. The production of erythropoietin in the grown artificial kidney precursor was confirmed by the same method as described in Transplantation VOL85, No11, June 15 2008, that is, immunostaining and genetic analysis of erythropoietin-producing cells....

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Abstract

Disclosed is an artificial kidney precursor comprising a non-human mammalian metanephron removed out of a living body, wherein the metanephron has been subjected to freezing and thawing in vitro and contains a mammalian mesenchymal stem cell transplanted thereinto in vitro. Also disclosed is a process for producing the artificial kidney precursor.

Description

Technical field [0001] The invention relates to an artificial kidney precursor and a preparation method thereof. Background technique [0002] The kidney is the main organ that produces erythropoietin; the reduction of erythropoietin production accompanying renal failure causes renal anemia as a complication. [0003] In recent years, a method has been proposed as a next-generation treatment method for renal anemia, in which an artificial kidney or its precursor having the potential to produce erythropoietin is prepared by regenerative medicine technology and transplanted to patients with renal anemia. For example, Patent Document 1 discloses a method for preparing erythropoietin-producing organoids (artificial kidney) precursors, which includes the following steps: transplanting isolated mammalian-derived mesenchymal stem cells into pregnant mammals An embryo in an animal host or an embryo isolated from a pregnant mammalian host to induce differentiation of mesenchymal stem cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A61K35/12A61K35/22A61K35/28A61K35/54A61L27/00A61L27/36A61L27/38A61P13/12
CPCC12N5/0686A61K35/12A61P13/12C12N5/0602A61L27/54A61L27/38A61F2/04A61K35/22C12N5/0697C12N2502/02C12N2506/1346C12N2523/00
Inventor 小林英司横尾隆甲斐耕太郎
Owner OTSUKA PHARM FAB INC
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