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Method for extracting sulforaphane from leaves of cauliflowers

An extraction method, the technology of sulforaphane, applied in the field of agriculture, can solve the problems of high cost and cumbersome steps, and achieve the effects of reducing production costs, simple extraction method, and simplified extraction steps

Inactive Publication Date: 2011-06-22
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nathan et al. (2001) proposed a method for the preparation of liquid chromatography for the simultaneous separation and purification of sulforaphane and sulforaphane nitrile from the degreased seeds of Brigadier cauliflower. However, the method has the problems of cumbersome steps and high cost

Method used

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  • Method for extracting sulforaphane from leaves of cauliflowers
  • Method for extracting sulforaphane from leaves of cauliflowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0030] (1) Weigh 1.0 g of cauliflower leaf dry powder, add 8 mL of exogenous myrosinase (enzyme activity unit is 1.0 units, prepared with 0.05 mM, pH=7.0 PBS buffer) and 4 mL of deionized water or ultrapure water (pH7.0-8.0), fully mixed on a vortex instrument to form a homogenate, and treated in a water bath at 40°C for 30 minutes to complete the enzymolysis reaction.

[0031] (2) Solvent extraction, filtration

[0032] After the enzymolysis reaction, according to the actual amount of exogenous enzyme and deionized water or ultrapure water 12mL, quickly (3min) add 120mL of dichloromethane to the homogenate, mix and shake well, extract for 15min, and mix according to the amount of leaf dry powder and The weight ratio of anhydrous sodium sulfate is 5.0~8.0 (W 叶片干粉 :W 无水硫酸钠 =5.0~8.0) add 6.0g of anhydrous sodium sulfate to dry and dehydrate, filter with medical absorbent cotton, and collect the filtrate.

[0033] (3) Concentration, vacuum freeze-drying

[0034] The filtrate ...

Embodiment approach 2

[0036] (1) Weigh 50.0g of cauliflower leaf dry powder, add 500mL of exogenous myrosinase (enzyme activity unit is 1.0units, prepared with 0.05mM, pH=7.0 PBS buffer) and 200mL of deionized water or ultrapure water (pH7.0-8.0), fully mixed on a vortex instrument to form a homogenate, and treated in a water bath at 40°C for 40 minutes to complete the enzymolysis reaction.

[0037] (2) Solvent extraction, filtration

[0038] After the enzymatic hydrolysis reaction, add 5.6L of dichloromethane to the homogenate quickly (3min) according to the actual amount of 700mL of exogenous enzyme and deionized water or ultrapure water, mix and shake well, extract for 20min, and mix according to the amount of leaf dry powder The weight ratio with anhydrous sodium sulfate is 5.0~8.0(W 叶片干粉 :W 无水硫酸钠 =5.0~8.0) Add 300.0g of anhydrous sodium sulfate to dry and dehydrate, filter with medical absorbent cotton, and collect the filtrate.

[0039] (3) Concentration, vacuum freeze-drying

[0040] The...

Embodiment approach 3

[0042] (1) Weigh 15.0g to 25.0g of cauliflower leaf dry powder, add 100 to 250mL of exogenous myrosinase (enzyme activity unit is 1.0units, prepared with 0.05mM, pH=7.0 PBS buffer) and 60 to 150mL of Deionized water or ultrapure water (pH 7.0-8.0) is fully mixed on a vortex instrument to form a homogenate, and treated in a water bath at 40°C for 30-50 minutes to complete the enzymatic hydrolysis reaction.

[0043] (2) Solvent extraction, filtration

[0044] After the enzymatic hydrolysis reaction, according to the actual amount of exogenous enzyme and deionized water or ultrapure water, quickly (3min) add 1000mL ~ 4000mL of dichloromethane to the homogenate, mix and shake well, extract for 20 ~ 30min, and follow The weight ratio of leaf dry powder and anhydrous sodium sulfate is 5.0~8.0 (W 叶片干粉 :W 无水硫酸钠 =5.0~8.0) add anhydrous sodium sulfate to dry and dehydrate, filter with medical absorbent cotton, and collect the filtrate.

[0045] (3) Concentration, vacuum freeze-drying...

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Abstract

The invention relates to a method for extracting sulforaphane from leaves of cauliflowers, which comprises the following steps of: after bulbs of the cauliflowers are harvested, immediately collecting waste leaves, cleaning, and performing freezing treatment in an ultralow temperature refrigerator; performing vacuum freeze drying, uniformly crushing into powder, taking 1 part of powder, adding exogenous myrosinase in an amount which is 6 to 10 times weight of the powder and deionized water in an amount which is 4 to 6 times weight of the powder, and fully mixing into uniform slurry; and performing enzymolysis reaction, solvent extraction, filtration, concentration, and vacuum freeze drying to obtain high-purity sulforaphane. The raw material sources are rich, the extraction method is simple, and the method is more suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to a process for separating and extracting active components in plants. Background technique [0002] Sulforaphane, also known as "Raphane", is a highly efficient chemical functional substance, which is obtained by hydrolysis of glucoraphanin (4-methylsulfinyl-butyl glucosinolate, RAA) by myrosinase or acid Isothiocyanate derivatives produced by hydrolysis, which are easily soluble in water, with a relative molecular weight of 177.3 and molecular formula C 6 h 11 S 2 NO. The basic structure is as figure 1 shown. [0003] A large number of experiments have proved that sulforaphane has a good preventive effect on esophageal cancer, breast cancer, lung cancer, colon cancer, etc., especially it can kill the Helicobacter pylori that causes gastric ulcer and most gastric cancers. Yuesheng Zhang reported that by feeding sulforaphane to newborn 6-day-old female mice, the production o...

Claims

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Application Information

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IPC IPC(8): C12P13/00
Inventor 乔勇进王海宏陈召亮
Owner SHANGHAI ACAD OF AGRI SCI
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