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Method for efficiently inducing jujube tree 2n pollen

A technology of pollen and jujube, which is applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problem of unsatisfactory induction effect, achieve the effect of simple and convenient method, increase the probability of occurrence, and high induction efficiency

Inactive Publication Date: 2012-02-01
HEBEI AGRICULTURAL UNIV.
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  • Application Information

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Problems solved by technology

According to the inventor of this patent, the inventor once tried to use the smear method to induce giant pollen, but the induction effect was not satisfactory; afterward, the minimally invasive technology was used to make the mutagen directly enter the jujube tree through the minimally invasive opening, and the induction efficiency of the high-activity 2n pollen was remarkable. improve

Method used

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  • Method for efficiently inducing jujube tree 2n pollen

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Embodiment Construction

[0014] Using the excellent diploid variety 'Honey Pot' as the test material, on May 12-13, 2010, a healthy jujube tree with normal development on the periphery of the canopy was selected, and the base was cut with a blade, with a length of about 0.8-1.2 mm and a depth of 0.8-1.2 mm. The xylem, the wound is smooth and has no burrs; quickly cover the wound with absorbent cotton, fix the absorbent cotton on the wound with plastic wrap to make the wound fully contact with the absorbent cotton, and wrap the plastic wrap with kraft paper for shading; use a syringe to inject 0.3% colchicine (containing 1% DMSO) was added dropwise on the absorbent cotton to soak the absorbent cotton; every 4-6 hours, the liquid medicine was dripped once to keep the absorbent cotton moist. After 24 hours, all wrappings were removed, and the wound was rinsed with water; collected and processed from June 1st to 7th. For flower buds that have reached the stage of bud splitting, use a dissecting needle to q...

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Abstract

The invention discloses a method for efficiently inducing jujube tree 2n pollen, which comprises the steps of: with colchicines as mutagen, firstly, cutting a jujube fruit-bearing shoot base part to be induced, covering a wound with absorbent cotton; and secondly, dropping the colchicines medicine liquid into the absorbent cotton to ensure that a jujube fruit-bearing shoot absorbs the medicine liquid into the xylem through the minimally invasive wound and conveys the medicine liquid upwards to stop spindle fibers in the young bud in a process of reductional division of pollen mother cells from forming and induce the pollen chromosome doubling so as to obtain the high-activity 2n pollen. The method is applied to the induction of the jujube high-activity 2n pollen, is simple and convenient,and has high induction efficiency.

Description

technical field [0001] The invention relates to a method for efficiently inducing high-vigority 2n pollen of jujube trees based on a minimally invasive technique. Background technique [0002] Jujube tree (Ziziphus jujuba Mill.) is a characteristic and dominant fruit tree native to my country, and it is the largest dry fruit tree species in my country. 'Zanhuang Dazao' is the only natural triploid jujube variety known so far, which has the advantages of large fruit, high yield and strong adaptability. Recently, the inventor of this patent used colchicine to mutate the diploid 'Linyi Lizao' and bred the first new tetraploid jujube variety 'Chenguang'. 'The performance is that the fruit is large and of high quality. Most of the cultivars of Ziziphus mauritiana Lam., a close relative of jujube, are tetraploid, and they all have large fruit and strong adaptability. Therefore, the ploidy breeding of jujube has broad prospects and has been paid more and more attention. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
Inventor 刘孟军刘平薛治慧
Owner HEBEI AGRICULTURAL UNIV.
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