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Method for improving anticancer activity of Codonopsis lanceolata Benth.et Hook.F

A technology of Radix Codonopsis radix, anticancer activity, applied in antitumor drugs, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem of no obvious inhibitory effect on tumor growth in mice

Inactive Publication Date: 2012-11-14
YANBIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after soaking Codonopsis vermicelli in a certain concentration of ethanol aqueous solution for 24 hours, the obtained extract had no obvious inhibitory effect on tumor growth in mice

Method used

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  • Method for improving anticancer activity of Codonopsis lanceolata Benth.et Hook.F
  • Method for improving anticancer activity of Codonopsis lanceolata Benth.et Hook.F
  • Method for improving anticancer activity of Codonopsis lanceolata Benth.et Hook.F

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Embodiment 1

[0059] Take dried Codonopsis pilosula slices, crush them, pass through a 20-mesh sieve, weigh 500g, add distilled water to soak, and sterilize under high pressure. When the temperature drops below 30°C, add 1% yeast and 1% glucose, and ferment at 30°C for 2 day, heated and refluxed with 50% ethanol for extraction, extracted at 0°C~100°C for 5 minutes to 24 hours, the filtrate was subjected to alcohol precipitation with 65% ethanol, concentrated under reduced pressure after recovering ethanol from the supernatant, and freeze-dried to obtain round Leaf Codonopsis extract, that is Codonopsis verticillum crude saponin.

Embodiment 2

[0061] Take 500g Codonopsis pilosula powder, add distilled water to soak, autoclave, add 2% yeast and 3% sucrose when the temperature drops below 30°C, ferment at 28°C for 7 days, extract with water at 70°C~100°C From 5 minutes to 24 hours, the filtrate is subjected to alcohol precipitation with 80% ethanol, and the supernatant is recovered from the ethanol, then freeze-dried, and separated with a macroporous adsorption resin to obtain a saponin mixture such as codonopsis codonopsis, syringin, and tangshenoside. Refining with liquid-liquid distribution technology and chromatographic technology to obtain Codonopsis saponins, which can be used to prepare anticancer drugs, food or tumor chemoprevention preparations.

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Abstract

The invention relates to a method for improving the anticancer activity for Codonopsis lanceolata Benth.et Hook.F, which comprises: slicing or homogenizing fresh Codonopsis lanceolata Benth.et Hook.F, or crushing dry Codonopsis lanceolata Benth.et Hook.F, or powdering the dry Codonopsis Lanceolata Benth.et Hook.F; soaking in water; sterilizing under a high pressure; adding 0.5 to 5.0 percent of active yeast and 1 to 8 percent of glucose under a sterile condition when the temperature lowers to 30 DEG C; fermenting at 20 to 37 DEG C for 1 to 10 days, and adding a polar solvent after fermentation to extract for 5 to 24 hours at 0 to 100 DEG C; and filtering, recovering solvent and concentrating under reduced pressure or drying by freezing. The method can improve the inhibition effect of Codonopsis lanceolata Benth.et Hook.F on the growth of tumors.

Description

technical field [0001] The invention relates to a method for increasing the anticancer active ingredient in Codonopsis verticillum, in particular to a method for increasing the content of the anticancer active ingredient in Codonopsis verticillum by using yeast fermentation technology. Background technique [0002] Codonopsis Lanceolata Benth.et Hook.F., also known as goat's milk, four-leaf ginseng, mountain carrot, mountain conch and white python meat, is the root of Codonopsis Lanceolata Benth.et Hook.F., which is mostly distributed in Northeast China , South China, Southwest China and North Korea, Japan, Russia and other countries, it is often eaten as a wild vegetable among the people. Traditionally, its root is used as medicine, sweet in taste and flat in nature. It is mainly used for symptoms such as physical weakness after illness, lack of milk, lung yin deficiency, carbuncle, breast carbuncle, sore and swelling. Modern pharmacological studies have shown that Codono...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/344A61P35/00A61K125/00
Inventor 韩春姬朴惠善李美子
Owner YANBIAN UNIV
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