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Method for producing polysaccharide by rice husk bran composite raw material and grifola frondosa mutant strain

A technology for mutagenizing strains and Grifola frondosa is applied in fungi, fermentation and other directions, can solve problems such as no reports, and achieve the effects of reducing production costs, saving raw material costs, and reducing land use

Inactive Publication Date: 2011-06-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another key problem of the present invention is to obtain the technological method of fermenting high-yield Grifola frondosa polysaccharides on rice bran bran composite medium by using mutagenic strains, and this research has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The strain of Grifola frondosa was the JSU10 strain obtained by mutagenesis. Use the plant hydrolase Viscozyme L produced by Novozymes, Denmark, with an enzyme activity of 1590.41IU / mL (FPU enzyme activity), add enzyme at 34U / g raw material, use a solid-liquid ratio of 1:15, and enzymatic hydrolysis time is 12h for rice bran Treat with bran to obtain the treatment solution as a medium for fermentation, the amount of rice bran used is 3g / 100mL medium, the amount of bran used is 3g / 100mL medium, add potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, pH is natural, the sample volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 26 o C, rotation speed 150r / min, culture time 7d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at...

Embodiment 2

[0017] Use plant hydrolase Viscozyme L produced by Novozymes, Denmark, with an enzyme activity of 1590.41IU / mL (FPU enzyme activity), add enzyme at 38FPU / g raw material, use a solid-liquid ratio of 1:25, and enzymatic hydrolysis time is 18h for rice bran Treat with bran to obtain the treatment solution as a medium for fermentation, the amount of rice bran used is 11g / 100mL medium, the amount of bran used is 11g / 100mL medium, add potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, pH natural, the sample loading volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 28 oC , rotation speed 180r / min, culture time 9d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a cert...

Embodiment 3

[0019] Use the plant hydrolase Viscozyme L produced by Novozymes, Denmark, with an enzyme activity of 1590.41IU / mL (FPU enzyme activity), add enzyme at 35FPU / g raw material, use a solid-liquid ratio of 1:18, and enzymatic hydrolysis time of 14h for rice bran Treat with bran to obtain the treatment solution as a medium for fermentation, the amount of rice bran used is 9g / 100mL medium, the amount of bran used is 5g / 100mL medium, and potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, pH natural, the sample loading volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 28 oC , rotation speed 150r / min, culture time 7d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a ce...

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PUM

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Abstract

The invention belongs to the fields of microorganism application and food biotechnology and discloses a method for producing polysaccharide by rice husk bran composite raw material and grifola frondosa mutant strain. The method comprises the following steps of: (1) treating rice husk and bran by adopting plant hydrolytic enzyme; (2) fermenting a grifola frondosa strain obtained by mutagenizing with the obtained treating fluid as a culture medium, wherein the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.4179; (3) carrying out centrifugal separation on mycelia obtained by liquid culture, washing the mycelia with distilled water, and drying to be constant weight to obtain the mycelia; (4) carrying out the conventional water extraction, deproteinization and ethanol precipitation on the mycelia to obtain intracellular polysaccharide; and (5) sequentially carrying out deproteinization and ethanol precipitation on the supernate obtained after fermentation liquor is centrifuged to obtain intracellular polysaccharide. By adopting the method disclosed by the invention, the production cost can be reduced, the material cost can be saved, less land can be used, the production cycle can be shortened, and grifola frondosa polysaccharide with high value and auxiliary anti-tumor curative effect can be produced.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a method for fermenting and high-yielding polysaccharides by using a grifola frondosa mutagenic strain suitable for growing on a medium in which rice bran and bran complexes are carbon and nitrogen sources. Background technique [0002] Scientific research at home and abroad and the domestic market have shown that edible fungus polysaccharides can enhance human immunity and assist in the treatment of tumors and other diseases, and have become an auxiliary product for tumor biotherapy. Domestic medicinal products made from the fruiting bodies of edible fungi include Ganoderma lucidum capsules from Wuhu, Anhui, and Grifola frondosa capsules from Qingyuan, Zhejiang. Non-pharmaceutical health products made from edible fungus extracts are also sold. Internationally, New Zealand produces "GanoPoly" polysaccharide extract series products, which are sold to the Uni...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/14
Inventor 刘伟民张建郭春梅徐立平任晓锋张志才顾慧敏杨锁华赵杰文
Owner JIANGSU UNIV
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