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Method for extracting grease from wet alga mud by using mixed organic solvent

A technology of organic solvents and mixed solvents, applied in the direction of fat oil/fat production and fat production, can solve the problems of large amount of alcohol, low solubility, low solubility, etc., to save drying energy consumption, high oil extraction rate, simple separation Effect

Inactive Publication Date: 2011-05-25
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this solution requires the use of alcohol solvents to dehydrate the wet algae mud. The amount of alcohol used is large, the energy consumption of alcohol recovery is high, and the extracted oil components are complex and difficult to separate and refine.
[0004] Lower alcohols are hydrophilic solvents, which can easily penetrate into algae cells, but lower alcohols have low solubility in oils at normal temperature and pressure, even under subcritical conditions.
Although lower alkanes have good solubility in oils, they have poor miscibility with water, making it difficult to extract oils by penetrating into the cells of wet algae

Method used

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  • Method for extracting grease from wet alga mud by using mixed organic solvent
  • Method for extracting grease from wet alga mud by using mixed organic solvent

Examples

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Effect test

Embodiment 1

[0027] Take Nannochloropsis wet algal mud 100g (water content 60%, initial total lipid content 33.4%), add the mixed liquid of the analysis pure ethanol (ethanol content 99%) of 400ml and normal hexane (both volume ratios are 1 / 6), be placed in the stainless steel pressure reaction kettle and seal, pass into N in the reaction kettle 2 Heat to 1.5MPa, heat to 110°C and maintain the reaction for 1 hour, then take out the algae pulp, centrifuge at 6500rpm for 10min, remove the microalgae cell residue, let the supernatant stand, separate the upper n-hexane phase, and use 60ml n-hexane for the ethanol phase Extracted twice, combined all n-hexane phases, concentrated by rotary evaporation, and dried to obtain 11.2 g of algae oil, with a total lipid extraction rate of 83.8%.

Embodiment 2

[0029] Take Nannochloropsis wet algae mud 100g (water content 60%, initial total fat content 33.4%), add the mixed liquid of the industrial ethanol (ethanol content 85%) of 400ml and normal hexane (both volume ratios are 1 / 3 ), placed in a high-pressure reactor and sealed, and passed into the reactor with N 2 Heat to 1.5MPa, heat to 110°C and react for 1 hour, then take out the algae slurry, centrifuge at 6500rpm for 10min, remove the microalgae cell residue, let the supernatant stand, separate the upper layer of n-hexane phase, and extract the ethanol phase with 100ml of n-hexane Once, all n-hexane phases were combined, concentrated by rotary evaporation, and dried to obtain 8.68 g of algae oil, with a total lipid extraction rate of 65%.

Embodiment 3

[0031] Weigh 100g of Nannochloropsis wet algae mud (water content 60%, initial total lipid content 33.4%), add 320ml of analytical pure ethanol and n-hexane mixed liquid (the volume ratio of the two is 1 / 5), and place under high pressure The reactor was sealed, and N was introduced into the reactor. 2 Heat to 1.5MPa, heat to 100°C and react for 1 hour, then take out the algae pulp, centrifuge at 6500rpm for 10min, remove the microalgae cell residue, let the supernatant stand, separate the upper n-hexane phase, and extract the ethanol phase with 60ml of n-hexane Twice, all n-hexane phases were combined, concentrated by rotary evaporation, and dried to obtain 10.69 g of algae oil, with a total lipid extraction rate of 80%.

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Abstract

The invention discloses a method for extracting grease from wet alga mud by using a mixed organic solvent. The method mainly comprises the following steps of: adding wet alga mud of microalgae which produce oil into a mixed solvent of a lower alcohol solvent and a lower paraffin hydrocarbon, heating in a reactor and introducing nitrogen, keeping the pressure and temperature of the reactor at a subcritical state and reacting; and performing liquid-solid separation and then cooling the obtained mixed liquid, collecting an upper-layer paraffin hydrocarbon phase, performing washing, extracting and phase splitting on a lower-layer alcohol-water phase by using lower paraffin hydrocarbon to obtain a paraffin hydrocarbon extraction phase, mixing the paraffin hydrocarbon extraction phase and the upper-layer paraffin hydrocarbon phase, performing evaporation concentration to obtain microalgae grease, and recovering paraffin hydrocarbon. Microalgae cells with high water content can be directly used, alga cells are not required to be dried and dehydrated, drying energy consumption is saved, the using amount of the extraction solvent is small, the extraction efficiency of the grease is high, the separation can be performed easily and the process is easy to amplify. The method has significance to the high-efficiency microalgae grease extraction with low energy consumption and the industrialization of microalgae bioenergy.

Description

technical field [0001] The invention belongs to the technical field of microalgae biochemical engineering, and in particular relates to a method for extracting oil from microalgae wet algae mud by using a mixed organic solvent. Background technique [0002] As the shortage of global fossil energy and the deteriorating environment become increasingly prominent, biodiesel, as a form of sustainable green energy, has attracted worldwide attention. At present, the raw materials used in the production of biodiesel are all from oil crops. Since the area yield of oil crops is not high, vigorous development of biodiesel will inevitably occupy a large amount of arable land and affect food production. Microalgae are the simplest and oldest lower plants, which can grow rapidly and synthesize a large amount of oil (mainly triglyceride) in the cells by directly using sunlight, CO2, N, P and other simple nutrients. , to provide new oil resources for biodiesel production. At present, it i...

Claims

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Application Information

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IPC IPC(8): C11B1/10
Inventor 陈晓琳陈闽刘天中王俊峰陈林张维彭小伟陈昱高莉丽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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