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Method for producing compound microbial preparation serving as fodder

A technology of composite microorganisms and production methods, which is applied in the field of microbial preparations, can solve the problems of short active shelf life, prone to autolysis, single bacterial species, etc., and achieves simple method, improved intestinal microecological balance, and strong stability. Effect

Inactive Publication Date: 2011-05-25
SICHUAN ACAD OF FOOD & FERMENTATION INDS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention aims at the deficiencies in the prior art, to provide a feed compound microbial preparation powder and a manufacturing method thereof. The preparation has defects such as single strain, low survival rate, poor stability, short active storage period and easy autolysis, which greatly improves the production and preservation rate of microbial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] [Example 1]: The production method of compound microorganism preparation for feeding, it comprises the following steps:

[0023] (1) Inoculate Bacillus subtilis species, Saccharomyces cerevisiae and Lactobacillus plantarum into Bacillus subtilis culture fluid, Saccharomyces cerevisiae culture fluid and Lactobacillus plantarum culture fluid respectively, collect the cells and make powder;

[0024] (2) Mix the Bacillus subtilis powder, Saccharomyces cerevisiae powder and Lactobacillus plantarum powder prepared in step (1) with ball-milled bran, bran and isomaltooligosaccharide, and produce powder-type feed after weighing and packaging. Using a compound microbial preparation, in its product, the percentage by weight of each component in the compound microbial preparation for feed is: 15% ball-milled bran, 84% bran, 0.02% isomaltooligosaccharide, wherein the number of Bacillus subtilis ≥ 2 ×10 7cfu / g, beer yeast count ≥5×10 6 cfu / g and the number of Lactobacillus plantar...

Embodiment 2

[0029] [Example 2]: The production method of compound microbial preparation for feeding is characterized in that it comprises the following steps:

[0030] (1) Inoculate Bacillus subtilis species, Saccharomyces cerevisiae and Lactobacillus plantarum into Bacillus subtilis culture fluid, Saccharomyces cerevisiae culture fluid and Lactobacillus plantarum culture fluid respectively, collect the cells and make powder;

[0031] (2) Mix the Bacillus subtilis powder, Saccharomyces cerevisiae powder and Lactobacillus plantarum powder prepared in step (1) with ball-milled bran, bran and isomaltooligosaccharide, and produce powder-type feed after weighing and packaging. Using a compound microbial preparation, in its product, the weight percent of each component in the compound microbial preparation for feed is: 25% of ball milled bran, 74% of bran, 1% of isomaltooligosaccharide, wherein the number of Bacillus subtilis is ≥ 2 ×10 7 cfu / g, beer yeast count ≥5×10 6 cfu / g and the number...

Embodiment 3

[0036] [Example 3]: The production method of compound microorganism preparation for feeding, it comprises the following steps:

[0037] (1) Inoculate Bacillus subtilis species, Saccharomyces cerevisiae and Lactobacillus plantarum into Bacillus subtilis culture fluid, Saccharomyces cerevisiae culture fluid and Lactobacillus plantarum culture fluid respectively, collect the cells and make powder;

[0038] (2) Mix the Bacillus subtilis powder, Saccharomyces cerevisiae powder and Lactobacillus plantarum powder prepared in step (1) with ball-milled bran, bran and isomaltooligosaccharide, and produce powder-type feed after weighing and packaging. Using a compound microbial preparation, in its product, the percentage by weight of each component in the compound microbial preparation for feed is: 20% ball-milled bran, 79.5% bran, 0.5% isomaltooligosaccharide, wherein the number of Bacillus subtilis ≥ 2 ×10 7 cfu / g, beer yeast count ≥5×10 6 cfu / g and the number of Lactobacillus plan...

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Abstract

The invention discloses a method for producing a compound microbial preparation serving as fodder, which comprises the steps of microbial inoculum production, material proportioning, bacillus subtilis medium preparation, beer yeast medium preparation and lactobacillus plantarum medium preparation. According to the method, bacillus subtilis, beer yeast and lactobacillus plantarum are bred to form compound strains, so that the totality of the compound thalli is increased, and the advantages such as high strain survival rate, strong stability and the like are achieved; the compound thalli are mixed with ball-milling chaff, bran and oligoisomaltose in a proper proportion, and the optimum compounding proportion is formed for the preparation; good conditions are created for the formation of the internal environment of intestinal canals of livestock and poultry, the aerobic bacillus subtilis interacts with yeast to generate more nutrient substances, amylase, protease cellulose and the like; and meanwhile, oxygen in the intestinal canals of livestock and poultry can be consumed, excellent growth environment is created for propagation of lactic acid bacteria, intestinal canal functions of livestock and poultry are improved, the fodder conversion rate is improved, and the microecological balance of the intestinal canals of livestock and poultry is improved.

Description

technical field [0001] The invention relates to a preparation method of a microbial preparation, in particular to a production method of a compound microbial preparation for feed using liquid submerged fermentation. Background technique [0002] There are hundreds of species and tens of billions of bacteria in the digestive system of animals, especially in the intestines, forming the intestinal flora, which is closely related to the health of animals. Intestinal flora can not only play a variety of nutritional and physiological functions, but also inhibit the proliferation of pathogenic bacteria and other defense against infection. The occurrence of colibacillosis, salmonellosis and Clostridium enteritis in livestock are closely related to the state of intestinal bacteria. Therefore, normalizing the balance of the intestinal flora is essential for maintaining animal health. It is closely related to the improvement and improvement of various indicators in livestock and poul...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/16C12N1/20C12N1/16C12R1/125C12R1/85C12R1/25A23K10/18A23K10/37A23K20/163
CPCY02P60/87
Inventor 康建平苏波黄静陈蓉周泽林岳晓敏谢文渊王拥军
Owner SICHUAN ACAD OF FOOD & FERMENTATION INDS
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