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Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor

A membrane bioreactor and anaerobic ammonium oxidation technology, which is applied in the directions of bacteria, aerobic and anaerobic process treatment, can solve the problems of low anammox bacteria enrichment efficiency and long enrichment time, and achieve the The effect of improving efficiency, shortening enrichment time, and rapid enrichment culture

Inactive Publication Date: 2011-05-18
HARBIN INST OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of long enrichment time of anammox reactor and low enrichment efficiency of anammox bacteria faced by the existing anammox process, a method for enriching and cultivating anammox bacteria by using membrane bioreactor is provided. Methods

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  • Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor
  • Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor
  • Method for enriching and culturing anaerobic ammonia oxidizing bacteria by using membrane bioreactor

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specific Embodiment approach 1

[0014] Specific embodiment one: the present embodiment utilizes the method for enriching and cultivating anammox bacteria in a membrane bioreactor, and is characterized in that the method for cultivating anammox bacteria using a membrane bioreactor as an enrichment reactor is realized in the following steps: 1. Add the simulated sewage medium into the water tank of the membrane bioreactor, and take the sludge from the anoxic biochemical pool of the A / O process of the urban sewage plant. Store anaerobically at room temperature for 3 to 5 days, and then inoculate the sludge in the anaerobic tank of the membrane bioreactor. The inoculation conditions of the sludge are 9920-9940 mg / L of total suspended solids and 4300-9940 mg / L of volatile suspended solids. 4340mg / L, the inoculum volume is 2-4L; 2. After inoculation, carry out enrichment culture in the membrane bioreactor. 7.5~8.5, the dissolved oxygen in the water is 0.06~0.09mg / L, the pH value in the anaerobic tank is 7.0~8.0, t...

specific Embodiment approach 2

[0015] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the temperature of the anoxic biochemical pool is 17-19 ° C when the sludge is taken in step 1, and the sludge is anaerobically stored at room temperature for 4 days, and the inoculum size is 3 L . Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0016] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that mud discharge in step two refers to discharging 50 mL of mud-water mixture every day during enrichment culture for 1 to 20 days; During enrichment culture for 1 to 20 days, stop the operation of the membrane bioreactor for 30 minutes every day, and settle the sludge in the membrane bioreactor for 30 minutes, then remove 75% of the supernatant liquid in the membrane bioreactor by siphon method, Supplement the simulated sewage culture medium with the same volume as the excluded liquid to the membrane bioreactor. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for enriching and culturing anaerobic ammonia oxidizing bacteria by using a membrane bioreactor, relating to a method for enriching and culturing anaerobic ammonia oxidizing bacteria and solving the problems of long enriching time and low enriching efficiency of the anaerobic ammonia oxidizing bacteria in the traditional anaerobic ammonia oxidation process. The method for enriching and culturing the anaerobic ammonia oxidizing bacteria by using the membrane bioreactor as an enriching reactor comprises the steps of: inoculating activated sludge of a sewage plant in the membrane bioreactor; and 2, enriching and culturing after inoculating. Compared with the traditional enriching method, the method for enriching and culturing the anaerobic ammonia oxidizing bacteria by using the membrane bioreactor realizes rapid enrichment of the anaerobic ammonia oxidizing bacteria, ensures that the enriching efficiency of the anaerobic ammonia oxidizing bacteria is improved by 80-95 percent, and can be widely applied to the biological denitrification technical field.

Description

technical field [0001] The invention relates to a method for enriching and cultivating anammox bacteria. Background technique [0002] Biological denitrification technology has developed rapidly in the past 20 years and has been widely used in production practice. The most widely used process is still the traditional digestion and denitrification process, but there are many problems in the traditional process, such as long process, large area, high infrastructure investment; in order to obtain higher sludge concentration and denitrification efficiency, sludge denitrification is required. The reflux and nitrification liquid reflux increase the power cost; adding alkali to neutralize the acidity during the nitrification process, and adding carbon sources to the denitrification process increases the operating cost; the activated sludge has weak resistance to impact load; it may cause secondary pollution, etc. [0003] Therefore, it is imperative to seek a new denitrification t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/30
Inventor 高大文侯国凤于英翠陶彧任南琪
Owner HARBIN INST OF TECH
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