Human osteosarcoma cell line group and mouse in-vivo transplantation model

Abstract

The invention discloses three human osteosarcoma cell lines well2, well 5 and LZJ, the collection numbers of the three human osteosarcoma cell lines are CCTCC C200961, CCTCC C200962 and CCTCC C200963 respectively, and the invention further discloses establishment of a corresponding NOD / SCID (nonobese diabetic / severe combined immunodeficiency disease) mouse in-vivo transplantation model, thereby providing a good and stable experimental subject and a model platform for clinical and fundamental research work of osteosarcoma. The established osteosarcoma cell lines can enable the in-vitro stable passage to be more than two years, have stable characters and enable morphology, have immuno-phenotype, chromosome genetics and other characteristics similar to the common osteosarcoma cell lines. The tumorigenesis of NOD / SCID mice is stable, the pathomorphism of tumor bodies is similar to primary tumor tissues of patients, and the animal model is simultaneously sensitive to common chemotherapeutic drugs for the osteosarcoma and can well simulate clinical retracking for clinical patients with the osteosarcoma.

Description

technical field [0001] The present invention relates to the field of microbial animal cell lines, more specifically, relates to the establishment of a group of human osteosarcoma cell lines and mouse in vivo transplantation models. Background technique [0002] Osteogenic sarcoma is a common bone malignancy, which occurs frequently in adolescents and has a high degree of malignancy. Currently, the commonly used treatment options are surgical treatment + neoadjuvant chemotherapy, but the curative effect is not very satisfactory, and the incidence of lung metastases is high. The basic reason is that the understanding of the pathogenesis of osteosarcoma is still unclear, and the basic research work is not in-depth. Osteogenic tumor cell lines and animal tumor xenograft models are basic tools commonly used in studies of tumor pathogenesis and drug and other therapy screening. Although the internationally authoritative cell bank ATCC has stored several osteosarcoma cell lines (...

AI Technical Summary

Problems solved by technology

These cell lines have been passaged in vitro for a long time, and some have lost some characteristics of the original isolated tumor tissue, especially their tumorigenic ability in animals is extremely low, and it is impossible to establish in vivo research models

Osteogenic sarcoma cell lines self-built by a small number of laboratories with tumorigenic ability are not widely used because of their unstable long-term passage traits, which limits the establishment of tumorigenic models of osteosarcoma in animals Basic research and screening of drugs and other therapies cannot be carried out in depth

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