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Radix Morindae officinalis extract, preparation method and applications thereof

A technology of extracts and drugs, which is applied in the field of new drugs, can solve the problems of antidepressant and anti-anxiety effects and applications of inulin-type 10-21 glycans that have not been seen, and inulin-types have not been found.

Active Publication Date: 2011-04-27
佗林医药(青岛)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After searching systematically, the inventor found no reports on the structure research of inulin-type 10-21 polysaccharides; no reports on antidepressant and anti-anxiety effects and applications of inulin-type 10-21 polysaccharides; no reports containing inulin Type 10-21 glycan extracts are reported as antidepressant and anti-anxiety effects and applications; there are no reports of inulin-type 10-21 glycans and pharmaceutical preparations containing their extracts as treatment of depression and anxiety diseases

Method used

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  • Radix Morindae officinalis extract, preparation method and applications thereof
  • Radix Morindae officinalis extract, preparation method and applications thereof
  • Radix Morindae officinalis extract, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] For each monomer compound and extract of formula A, the dosage range for normal people is 0.1-40 mg / kg body weight / time, preferably 0.2-20 mg / kg body weight / time, based on the conversion of the body surface area of ​​animals and humans. Example 1 Preparation of inulin-type 10-21 polysaccharides from Morinda officinalis

[0019] Take 500 grams of Morinda officinalis crude powder, heat and extract with 6 times the amount (W / W) of 30% ethanol, extract 4 times, each time for 1 hour, filter, combine the filtrates, concentrate to relative density D=1.05~1.10 ( 50 ℃) solution, put it at room temperature, filter, pass through a chromatographic column prepacked with activated carbon (the ratio of activated carbon to medicinal material is 1 / 1, W / W), first elute with 3% ethanol, and then use 50 % ethanol was eluted, and detected by high performance liquid phase (HPLC, system and conditions see Example 2), the fractions containing inulin-type 10-21 polysaccharides were collected, t...

Embodiment 2

[0024] Example 2 Preparation of inulin-type 10-21 polysaccharides from Jerusalem artichoke and Jerusalem artichoke

[0025] Get 500 grams of dried coarse powder of medicinal material Jerusalem artichoke, extract according to the extraction conditions of Example 1, obtain the Jerusalem artichoke extract containing formula A, and the yield is 10% to 30%; take another 500 grams of dried coarse powder of Jerusalem artichoke, and extract according to Carry out extraction under the extraction conditions of Example 1 to obtain the chrysanthemum yam extract containing formula A, with a yield of 8% to 20%;

[0026] According to the separation conditions of Example 1, the Jerusalem artichoke extract and the Jerusalem artichoke extract were separated and collected according to the peaks, and inulin-type 10-21 polysaccharides were also obtained. Figure three and picture Four.

[0027]

[0028]

Embodiment 3

[0029] Example 3 Structural identification of inulin-type 10-glycan

[0030] Inulin-type 10-glycan is a white solid, [α] 20D -19.5° (C=1.0, H 2 O). IRλmax cm -1 : 3425 (br s, OH), 2935, 2815, 1463 (w), 1085 (s), MALDI-TOF-MS m / z: 1638. 1 H-NMR (D 2 O)δ: 5.41 (1H d, J=3.7Hz; Glucose 1-H), 4.18~4.28 (multiple H, all are d peaks, J=8.2~8.3Hz, all are fructose 3-H), 3.85~4.15 (Multiple H, all are dd peaks, J=9.2~9.5Hz, all are fructose 4-H). 13 C-NMR (D 2 O) δ: 94.2 (glucose 1-C), 73.2 (glucose 2-C), 74.3 (glucose 3-C), 71.0 (glucose 4-C), 74.2 (glucose 5-C), 62.1 (glucose 6-C ). go through 1 H- 1 H COZY, 1 H- 13 C COZY, HMBC, HMQC comprehensive analysis, hydrolysis of inulin type 10 glycans, gas chromatography analysis (see Example 5 for the method), shows that the molar ratio of glucose to fructose is 1:9. Referring to the structural characteristics of such compounds (Cui Chengbin et al., "Inulin-type oligosaccharides 1 HNMR and 13 CNMR Research", Chinese Journal ...

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Abstract

The invention provides a Morinda officinalis extractive and a preparation method and an application. The invention provides a preparation method of an extract from a traditional Chinese medicine Radix Morindae officinalis. The invention provides preparation methods and structures of 12 new compounds in the extract (such as synanthrin type 10-21 polysaccharide and the like), and the molecular connection mode is as follows: alpha-D-glucopyranose-(1->2)-[beta-D-fructofuranose-(1->2)]8-19-beta-D-fructofuranose. The invention provides methods for preparing these compounds from Helianthus tuberosus, yacon and other plants. The invention provides a method for preparing the extract into a medicinal composition. The invention also provides applications of the extract used as an antidepressant and an anxiolytic.

Description

technical field [0001] The present invention relates to an inulin-type 10-21 polysaccharide compound or an extract containing them. Such compounds are present in the extracts of traditional Chinese medicines Morindae officinalis (Radix Morindae officinalis), Jerusalem artichoke (Helianthus tuberosus l.) and Jerusalem artichoke (yacon). Further, these compounds or their extracts are prepared into medicines with auxiliary materials. It belongs to the field of new drugs. technical background [0002] In recent years, it has been found that Morinda officinalis How. of Rubiaceae (Rubiaceae) has antidepressant effect. Cai Bing, Cui Chengbin, etc., "Evaluation of the three-stage combination test of the antidepressant effect of the traditional Chinese medicine Morinda officinalis in rats and mice", Chinese Journal of Pharmaceutical Sciences of the People's Liberation Army, 2005, Vol. Drugs have good antidepressant effects on a variety of animal models; Cui Chengbin, Cai Bing, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/716A61P25/24
Inventor 黄桂兰王鲁明王楠王佳赵炎杨暖
Owner 佗林医药(青岛)有限公司
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