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Abalone breeding method capable of saving energy and reducing emissions

An energy-saving and emission-reducing technology for abalone, which is applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., to achieve the effects of improving the physique of abalone seedlings, good safety, and improving the survival rate

Inactive Publication Date: 2011-04-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding method of abalone seedlings using Bdellovibrio bdelloplasts has not been reported at home and abroad.

Method used

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  • Abalone breeding method capable of saving energy and reducing emissions
  • Abalone breeding method capable of saving energy and reducing emissions
  • Abalone breeding method capable of saving energy and reducing emissions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Bdellovibrio Bdeloidoplasts

[0037] The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a transparent circular phage plaque with a diameter of 2-3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. Fermentation method of hiruplasma Fermentation: Inoculate 0.5mL10 in conical flask with 100mL nutrient broth (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) 7 cfu / mL Vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from American Type Culture Collection, American Type Culture Collection, No.: ATCC 17802), 200rpm, 28 ℃ shaker culture for 18 hours, the culture solution was centrifuged at 4 ℃, 5000rpm for 15min , with 5mL normal saline to suspend and precipitate the bacteria, and then add it to a liquid medium containing 100mL DNB (dilute nutrient broth) (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, sea crystal 28g, dissolved in 1000mL distilled water, pH ...

Embodiment 2

[0039] 1. Application of Bdellovibrio brucoplasma in seedling cultivation

[0040] The present invention can be divided into two stages altogether. The first stage is algae cultivation. In the nursery pond, sand-filtered and sediment-treated seawater is injected and aerated. The attachment base is polyethylene corrugated board. The algae cultivation time is 40 days. No Bdellovibrio biliplasma is added in this stage. The second stage is seedling cultivation, and the second stage is the seedling raising stage. The content of the present invention is mainly carried out at this stage. After cultivating algae for 40 days, begin to cloth abalone fertilized eggs in the seedling pond, and the seedling time is 40 days. The experimental group of this stage Add Bdellovibrio bdelloplasts immediately after each water change. The Bdellovibrio bdelloplasts obtained in Example 1 were injected with different concentrations of Bdellovibrio bdelloplasts into the cultured water body at different...

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Abstract

The invention discloses an abalone breeding method capable of saving energy and reducing emissions, comprising the following steps of: (1) cultivating algae; (2) breeding seedlings: adding bdellovibrio bacteriovorus leech plastid bacterial liquid during breeding so that the concentration of the leech plastid in water body is at least up to 10pfu / mL, wherein the bdellovibrio bacteriovorus strain is Bdellovibrio sp. BDFM05 which is preserved by China Center for Type Culture Collection on August 7th, 2009, with preservation number of CCTCC NO: M 209172; (3) exchanging water: exchanging the water every 5-30 days after egg distribution, and immediately adding the bdellovibrio bacteriovorus leech plastid bacterial liquid after exchanging water; (4) collecting products. The bdellovibrio bacteriovorus leech plastid has strong effect of eliminating common bacteria in cultivating environment. Since the bdellovibrio bacteriovorus leech plastid has no toxic and side effect to the abalone, the bdellovibrio bacteriovorus leech plastid is adaptable for industrial breeding of abalone in large scales and is capable of improving survival rate of the abalone during breeding.

Description

technical field [0001] The invention relates to a method for raising seedlings of aquaculture shellfish, in particular to a method for promoting rapid growth of abalone seedlings, enhancing immunity and improving the survival rate of abalone seedlings. Background technique [0002] Abalone is a kind of marine shellfish, also known as "Shicai Ming", and its scientific name is "Haliotis". The meat of abalone is delicious and has high nutritional value. The curative effect of abalone meat is good for intestines, nourishing yin and strengthening kidney. The shell has the function of improving eyesight and lowering blood pressure. The abalone extracted from abalone has obvious effect on inhibiting tumors. Therefore, abalone is not only a nutritious food but also a rare marine drug. Recently, Australia and other countries have successfully cultivated pearls in abalone, which has further improved the economic benefits of the abalone industry. my country is one of the first countri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80A61K35/741A61P31/04A61P37/04C02F3/34
CPCY02A40/81
Inventor 蔡俊鹏蒋小平
Owner SOUTH CHINA UNIV OF TECH
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