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Method for displaying animal (bovine) heart conducting system

A technology of conduction system and heart, which is applied in the field of casting to display the three-dimensional structure of animal heart conduction system, which can solve the problems of inability to inject fillers, etc., and achieve the effect of facilitating the walking position and distribution of the general structure, easy to operate, and not easy to fade

Inactive Publication Date: 2011-03-30
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After more than 30 cases of bovine heart injection research, the last third of the experiments were all successful, which solved the problem that the filling agent could not be injected into the heart system, thus completing the present invention

Method used

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  • Method for displaying animal (bovine) heart conducting system
  • Method for displaying animal (bovine) heart conducting system
  • Method for displaying animal (bovine) heart conducting system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] [Example 1] Dissection method

[0016] 1. Preparation of general materials Fresh beef heart, 5-10% liquid green plastic filler (recipe: solute: perchlorethylene 5-10g; solvent: cyclohexanone 100ml; shrinkage agent: dibutyl phthalate 3 ~5ml; 0.5~1.0g of Shanghai Marley Chlorine Oil Paint (Model: E1387), add the solute into the solvent, let it stand for 2 days to fully dissolve, then add shrinkage agent and Chlorine Oil Paint, and stir while adding , fully dissolved before use), 5 ~ 10ml syringes and needles, high-quality carbon ink, general scalpel instruments, etc.

[0017] 2. The right ventricle is cut from the anterior wall of the pulmonary artery to the conus pulmonary artery, then cut parallel to the right along the right coronary artery to the right heart border, and then cut along the right heart border to the apex of the heart. For the left ventricle, enter the knife from the midline of the anterior and posterior papillary muscles, cut to the bottom and apex of ...

Embodiment 2

[0018] [Example 2] perfusion method

[0019] 1. Take out the beef heart that has been refrigerated for 24 hours, and rinse it under running water for 80-100 minutes.

[0020] 2. Carefully look for the grayish-white or slightly transparent filament structure under the endocardium, or identify the Purkinje fibers after foaming the endocardium with an iodine cotton swab.

[0021] 3. Hold the syringe fully filled with the prepared plastic filler, and press the needle tip of the syringe parallel to the endocardium with the filamentous structure with a little force, so that the endocardium is slightly depressed, while keeping the slope of the needle tip upward. Advance the needle so that the bevel of the needle tip completely enters the subendocardium, and then advance the needle 1 to 2 mm. Turn the syringe 180 smoothly so that the bevel of the needle tip is directed toward the myocardium.

[0022] 4. Then try to push the piston lightly, and the filler can enter by pushing lightly...

Embodiment 3

[0024] [Example 3] Corrosion flushing

[0025] The perfused heart was allowed to stand naturally for 48 hours, and then it was moved to 50% hydrochloric acid solution for corrosion. After 72 hours, take it out and wash it with running water to wash away the decayed cardiac tissue, and keep the blue plastic cast bracket of the cardiac conduction system (left and right bundle branches and Purkinje fibers).

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Abstract

The invention belongs to the technical field of biomedicine, and provides a general casting technique for researching a heart conducting system. Proved by the experiments of dissolving perchloroethylene plastic into an organic cyclohexanone solvent, adding shrinkage increasing agent and green oil paint into the solvent to prepare a filling agent, injecting the filling agent into a tissue tunica vaginalis of the heart conducting system and performing acid corrosion and running water flushing, the technique not only can display the traveling positions and the distribution characteristics of the left and right bundle branches of the heart conducting system under the endocardium, but also comprehensively displays the characteristics and the three-dimensional structures of the left and right bundle branches among the myocardium tissues. The technique makes up the general morphology for researching the heart conducting system.

Description

technical field [0001] The invention relates to the biological technology of the heart conduction system, and is a casting technique for displaying the three-dimensional structure of the animal heart conduction system. Background technique [0002] The cardiac conduction system is composed of specially differentiated cardiomyocytes, which mainly generate and conduct excitation and control the rhythmic activity of the heart. At present, most of the studies on the cardiac conduction system at home and abroad are observational studies on histology, electrocardiography, and imaging, while there are few studies on the general shape of anatomy. In the early 1960s, James et al. conducted research on heart conduction in humans and animals, and proposed that there are three special conduction bundles connected between the sinoatrial node and the atrioventricular node, which are called the anterior, middle, and posterior interstitial bundle connections. But so far, no one has proved ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/28
Inventor 申彪张疆莉徐萍尹延彦郭志坤
Owner XINXIANG MEDICAL UNIV
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