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Detection method and detection test paper for beta-lactamase

A lactamase and detection method technology, applied in the detection field, can solve the problems of high false negative rate and long consumption time of beta-lactamase, and achieve the effects of stable results, simple operation and improved sensitivity

Active Publication Date: 2011-03-30
南京星福星医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to overcome the defects of high false negative rate and long time consumption in the detection of β-lactamase in the prior art, and provide a highly sensitive, convenient and quick detection method of β-lactamase

Method used

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  • Detection method and detection test paper for beta-lactamase
  • Detection method and detection test paper for beta-lactamase
  • Detection method and detection test paper for beta-lactamase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: Preparation of β-lactamase detection test strip

[0056] structure see figure 1 shown. 1 is a supporting material, and 2 pasted on it is a color test paper sheet, which is packaged and labeled with a light-proof material for external use.

[0057] Preparation of chromogenic test strips: The chromogenic agent is prepared with a diluent so that the final concentration is 0.01-1 mg / ml. After the filter paper is cut to an appropriate size, the color test paper can be in various shapes such as round, rectangular, etc., with an area of ​​0.2 to 1.5 cm 2 It is appropriate. Wet with chromogenic solution, dry. The drying method can be 30-80 ℃ short-term drying, preferably freeze-drying. The dried paper is pasted on the supporting material, packaged in a dark place, and stored at a low temperature and away from light. Use within 6 months.

[0058] Color test strips can be in various shapes such as round, rectangular, etc., with an area of ​​0.2 to 1.5 cm 2 I...

Embodiment 2

[0059] Example 2: Preparation of β-lactamase multi-arm detection test paper (taking 6 arms as an example)

[0060] structured as figure 2 Shown: A-F: numbering of the six arms.

[0061] Each arm is divided into 4 areas: 1 is the label area, which is used to mark the arm number and substrate; 2 is the chromogenic area, containing cefuronan; 3 area is the substrate area to be tested, and the filter paper contains the substrate to be tested; 4 The area is the sampling area of ​​the central area.

[0062] Size: Each arm is 2-4cm long and 0.5-1.5cm wide.

[0063] Zone 4: 0.5-1.0 cm in diameter; 0.5-1.5 cm in width.

[0064] Zone 3: length 0.5-1.5cm; width 0.5-1.5cm.

[0065] Zone 2: 0.3-1.5 cm long and 0.5-1.5 cm wide.

[0066] Zone 1: 0.5-1.5 cm long and 0.5-1.5 cm wide.

[0067] Indicate the content in zone 1: arm number, Chinese or English abbreviation of the drug in zone 3 of the arm.

[0068] Step 1: Make the medicated paper:

[0069] Concentration requirements for al...

Embodiment 3

[0079] Embodiment 3: the preparation of kit of the present invention

[0080] What the kit includes:

[0081] 1. Bacteria amplification bottle, containing bacterial rapid expansion medium powder, stored at 4-8°C.

[0082] 2. 1.0mL EP tube (containing one or more of the emulsifying agent lysozyme, brevicin, and polymyxin), and store at 4-8°C.

[0083] 3. Prepare the β-lactamase detection test strips in Example 1, and store them in the dark at -20°C.

[0084] 4. The multi-arm test paper for β-lactamase type detection prepared in Example 2, stored at -20°C in the dark.

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Abstract

The invention relates to the field of medicaments and provides a detection method for beta-lactamase. The detection method comprises the following steps of: culturing a sample which may contain microorganisms for 2 to 24 hours; and performing beta-lactamase detection on the sample by a chromogenic cephalosporin method. The invention also provides a method for detecting substrate characteristics of the beta-lactamase based on a substrate advantage combination principle. The method of the invention can not only judge whether the sample contains bacteria for producing the beta-lactamase rapidly,but also judge the medicament resistance of the bacteria to beta-lactam antibiotics. The invention also provides detection test paper for the beta-lactamase and a kit comprising the test paper. Compared with the prior art, the detection method and the detection test paper of the invention have the advantages that: the operation is simple and convenient, the detection speed is high, the response is sensitive and the result is stable; therefore, the detection method and the detection test have an extensive clinical application prospect.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of β-lactamase and a detection test paper. Background technique: [0002] Increasing bacterial resistance has become a global problem. There are many aspects to the mechanism of bacterial drug resistance, and β-lactamase is the most common mechanism of resistance to β-lactam antibiotics in Gram-negative bacilli. β-lactamase has been discovered before the application of β-lactam antibiotics in clinical practice. It is not necessary for bacterial growth, and its only function is to hydrolyze the β-lactam ring. [0003] Thousands of β-lactamases have been discovered so far, which widely exist in clinical drug-resistant bacteria. There are many classifications of β-lactamases, which can be divided into five categories according to their biochemical characteristics: [0004] The first type is cephalosporin hydrolase (ie, Amp-C enzyme), and the producing bacteria are mainly G...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
Inventor 李苌清孙明杰王霆
Owner 南京星福星医药科技有限公司
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