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Bacillus thuringiensis microbial insecticide, preparation method and special culture medium thereof

A microbial insecticide, the technology of Bacillus aureus, applied in the field of environmental engineering, can solve the problems that Bacillus thuringiensis microbial insecticides have not yet been seen, and achieve the effect of expanding resource utilization, high added value, and promoting popularization and application

Inactive Publication Date: 2013-03-27
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to domestic and foreign literature and patent searches, there is no report on the use of municipal sludge as raw material to ferment Bacillus thuringiensis microbial insecticides

Method used

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  • Bacillus thuringiensis microbial insecticide, preparation method and special culture medium thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, preparation and detection of Bacillus thuringiensis microbial insecticide

[0033] One, the preparation of Bacillus thuringiensis microbial insecticide

[0034] The preparation process of bacillus thuringiensis microbial insecticide is as follows figure 1 shown.

[0035] 1. Prepare culture medium

[0036] 1) Take municipal sludge samples

[0037] The mixed sludge from the sewage treatment plant was taken for experiment. The sewage treatment of the plant adopts the activated sludge method, which produces a large amount of primary sludge and residual activated sludge. After analysis, the carbohydrate content in the sludge is 104g / kgTS, the protein content is 300g / kgTS, the phosphorus content is 4g / kgTS, and the pH = 6.8.

[0038] 2) Medium conditioning

[0039] Adjust the solid content of municipal sludge to be 30g / L, add dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate and foam enemy, so that the final concentration of dipotassium hy...

Embodiment 2

[0054] Embodiment 2, preparation and detection of bacillus thuringiensis microbial insecticide

[0055] One, the preparation of Bacillus thuringiensis microbial insecticide

[0056] 1. Prepare culture medium

[0057] 1) Take municipal sludge samples

[0058] The thickened sludge from the sewage treatment plant was used for the experiment. The sewage treatment of the plant adopts the activated sludge method, and the remaining activated sludge enters the thickening tank, and the concentrated sludge is taken from the bottom of the tank. After analysis, the carbohydrate content in the sludge is 123g / kgTS, the crude protein content is 334g / kgTS, and the phosphorus content is . 7g / kgTS, pH=6.5.

[0059] 2) Medium conditioning

[0060] Adjust the solid content of municipal sludge to 20g / L, add dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate and foam enemy, so that the final concentration of dipotassium hydrogen phosphate is 0.4g / L, the final concentration of ...

Embodiment 3

[0076] Embodiment 3, preparation and detection of bacillus thuringiensis microbial insecticide

[0077] One, the preparation of Bacillus thuringiensis microbial insecticide

[0078] 1. Prepare culture medium

[0079] 1) Take municipal sludge samples

[0080] Take the remaining activated sludge from the sewage treatment plant, which uses the oxidation ditch process to treat domestic sewage. In order to ensure a higher sludge solid content, the residence time of the sludge in the sedimentation tank must be more than 10 days. After analysis, the carbohydrate content in the sludge is 150g / kgTS, the crude protein content is 380g / kgTS, the phosphorus content is 10g / kgTS, and the pH=6.7.

[0081] 2) Medium conditioning

[0082] Adjust the solid content of municipal sludge to 50g / L, add dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate and foam enemy, so that the final concentration of dipotassium hydrogen phosphate is 1.0g / L, the final concentration of magnesiu...

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Abstract

The invention discloses a bacillus thuringiensis microbial insecticide, a preparation method and a special culture medium thereof. The culture medium is prepared by mixing sludge, mineral salts and a defoamer and sterilizing. In the preparation method, bacillus thuringiensis is put in the culture medium and is subjected to fermentation culture so as to prepare the bacillus thuringiensis microbial insecticide. By the method of the invention, the production cost of the microbial insecticide is reduced, the additional value of products is high, and the defect of generating stink in the process of fermentation is overcome.

Description

technical field [0001] The invention relates to the field of environmental engineering, in particular to a bacillus thuringiensis microbial insecticide, a preparation method and a special culture medium. Background technique [0002] Municipal sludge is the sediment inevitably produced in the process of sewage treatment. The irregular disposal of sludge has become a new hidden danger in today's urban environment. How to properly dispose of these increasingly large amounts of organic solid waste is one of the most troublesome problems in sewage treatment plants. Generally speaking, the cost of sewage treatment and disposal accounts for about 30-40% of the total cost of sewage treatment. The current disposal methods of sludge landfill, land use, and incineration all have their own insurmountable defects, and cannot meet the actual needs of current resource utilization. [0003] In fact, municipal sludge contains a large amount of nutrients such as carbon, nitrogen, phosphor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/07
Inventor 常明周顺桂倪晋仁
Owner PEKING UNIV
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