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Mussel polysaccharide and preparation method and application thereof

A polysaccharide and mussel technology, which is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of difficult separation in the separation process, reducing the content of polysaccharides in extracts, and increasing the difficulty of separation and extraction.

Active Publication Date: 2011-03-16
ZHEJIANG AINUO BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When preparing mussel polysaccharides, the mussel meat is usually extracted by heating water and heated at a high temperature. The reaction conditions are very severe, which often causes the mussel meat to decompose many impurities, and the polysaccharide molecular chain will also break, causing the active ingredients to change and degrade. It is also often difficult to separate during the separation process, which increases the difficulty of separation and extraction and reduces the content of polysaccharides in the extract

Method used

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  • Mussel polysaccharide and preparation method and application thereof
  • Mussel polysaccharide and preparation method and application thereof
  • Mussel polysaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 prepares mussel polysaccharide

[0034] (1) Take 1000kg of fresh river mussel meat, place it in a meat grinder, grind it, and put it into a storage tank.

[0035] (2) Add 1500 L of 95% ethanol to the storage tank containing mussel meat, stir evenly, add until the ethanol concentration reaches 70-75%, and leave it for 24 hours.

[0036] (3) The mussel meat soaked in ethanol is put into a filter press for pressure filtration, and the pressurization speed is 0.1Mpa / 30min, and the filter residue is recovered.

[0037] (4) Put the filter residue in a storage tank, add 1500L of water twice the volume of the filter residue, and stir for 0.5-1h.

[0038] (5) The material in the step (4) is squeezed into the filter press for pressure filtration, and the pressurization speed is 0.1Mpa / 30min, and the filtrate is recovered.

[0039] (6) The filtrate was spray-dried to obtain 3 kg of mussel polysaccharide. The inlet temperature of the spray dryer is 180°C and the out...

Embodiment 2

[0045] Example 2 Main pharmacodynamic test data of mussel polysaccharide

[0046] 1. Mouse swimming test

[0047] (1) Experimental materials

[0048] 1. The drug clam polysaccharide is provided by our company: the sample of Example 1 is made into a solution of the required concentration with purified water. Ginseng extract, 1ml is equivalent to 1g ginseng, and the purified water produced by Hangzhou No. 2 Traditional Chinese Medicine Factory is used to make a solution of the required concentration.

[0049] 2. Animal health Kunming mice, weighing 20±1g, half male and half male, were provided by the animal room of Zhejiang Provincial Drug Control Institute.

[0050] (2) Experimental method

[0051]1. Grouping with administration The mice were randomly divided into 5 groups according to gender and weight balance (see Table 1): blank control group, treated with purified water instead of administration; ginseng control group 3g / kg; mussel polysaccharide 3.6g / kg, 1.8g / kg and 0...

Embodiment 3

[0094] Analgesic effect of embodiment 3 mussel polysaccharide

[0095] Experimental Materials

[0096] 1. Drugs

[0097] Mussel polysaccharide: powder obtained by drying mussel meat extract, provided by our company (Example 1). Dissolve with purified water just before use.

[0098] Morphine: 10mg / ml, Shenyang No. 1 Pharmaceutical Factory.

[0099] 2. Animals

[0100] PIH mice were provided by Zhejiang Experimental Animal Center

[0101] Method and Results

[0102] 1. Effects on mice acetic acid writhing test

[0103] NIH mice, 18-25g, both male and female, were randomly divided into 5 groups, 10 in each group. The treatment group was given mussel polysaccharide 4, 2, 1 g / kg by intragastric administration, the control group was given equal volume of water, and the morphine group was given intraperitoneal injection of 10 mg / kg of morphine. Take the medicine for 1 hour (30 minutes after the intraperitoneal injection of morphine), inject 0.7% acetic acid 0.1ml / 10g into the...

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Abstract

The invention provides a mussel polysaccharide. The polysaccharide content of the mussel polysaccharide is detected by an ultraviolet spectrophotometer and the polysaccharide is 55 to 90 percent based on glucose (C6H12O6). Monosaccharide components in the mussel polysaccharide, which are detected by high performance liquid chromatography, comprise the glucose, glucuronic acid, glucosamine and the like. The mussel polysaccharide is prepared by alcohol treatment, water extraction, pressure filtration, drying and other processes. Clinical verification results show that the mussel polysaccharide has definite curative effect of treating patients with syndrome of endogenous heat due to yin deficiency and the syndrome of endogenous heat due to yin deficiency accompanied with cancer pain, is convenient to take and safe, does not have any toxic or side effect and can be used for preparing medicaments for nourishing yin, clearing heat, diminishing swelling and relieving pain and the cancer pain.

Description

technical field [0001] The invention relates to biological preparations, in particular to mussel polysaccharide and its preparation method and application. Background technique [0002] River mussel, known as ubei in ancient times, is the meat and secretions of mussels such as mussels such as mussels in the family Musselidae. It has a sweet, salty and cold taste. There are records of mussels being used as medicine in ancient herbal medicines of all dynasties. Zhao Xuemin in the Qing Dynasty said in "Compendium of Materia Medica Supplements" that "the water contained in the shell and the essence of the true cloudy sky are the most widely used medicines." The Tang Dynasty Chen Zangqi's "Supplements to Materia Medica" claimed that it can "improve eyesight and dehumidify, and treat women's consumptive blood". Song "Rihuazi Materia Medica" said that it has the functions of "improving eyesight, quenching thirst, eliminating annoyance, detoxifying heat, treating women's blood met...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P29/00
Inventor 陆方明吴恒
Owner ZHEJIANG AINUO BIOLOGICAL PHARMA CO LTD
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