Application of bdellovibrio sp. telotroch to prevention and control of bacteria in sashimi making process
A production process and technology of leech vibrio, applied in the field of disinfection and sterilization, and leech vibrio liquid, can solve the problems of short shelf life, incomplete cleaning, easy contamination of bacteria, etc. Effect
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Embodiment 1
[0039] The application of Bdellovibrio swimmers in controlling the total number of colonies and pathogenic bacteria during the initial washing of raw raw materials for sashimi. The following sashimi takes salmon as an example.
[0040] The application method includes the following specific steps and their conditions:
[0041] Step 1: Preparation of Bdellovibrio Swimming Body Bacterial Liquid
[0042] The Bdellovibrio swimming body is fermented by the fermentation method of the high-density Bdellovibrio swimming body disclosed in the national invention patent application application number "200710031166.X": 100mL MRS liquid medium (peptone 10g, beef extract powder 8g, Yeast extract powder 4g, glucose 20g, dipotassium hydrogen phosphate 2g, triammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.2g, manganese sulfate 0.05g, 1g Tween 80, pH value 6.0~6.4) in a conical flask Inoculate 2mL with a concentration of 10 10 Streptococcus lactis (Streptococcus lactis, purchased ...
Embodiment 2
[0058] Application of Bdellovibrio swimming body in the disinfection and sterilization of sashimi production console and processing workshop
[0059] The test consisted of the following specific steps and their conditions:
[0060] Step 1: Same as Step 1 of Example 1
[0061] Step 2: Disinfection and sterilization experiment of applying Bdellovibrio swimmers to sashimi production operation table and workshop.
[0062] Adopt the application method of the present invention, select the operating table and the workshop that are the same in size and specifications, be divided into a plurality of different test groups and control groups, wherein three control groups are three parallel samples, and the rest are test groups, three for each group, for three parallel samples. Measure the total number of colonies and the pathogenic bacteria content of the test group and the control group respectively, then on the surface of the test group processing sashimi operation table and workshop...
Embodiment 3
[0065] Swimming body of Bdellovibrio applied to the disinfection and sterilization of sashimi serving dishes
[0066] The test consisted of the following specific steps and their conditions:
[0067] Step 1: Same as Step 1 of Example 1
[0068] Step 2: Disinfection and sterilization experiments using Bdellovibrio swimming bodies on sashimi dishes.
[0069] Adopt the application method of the present invention, be divided into a plurality of different test groups and control groups, select the same dish of specification and size, wherein three control groups are three parallel samples, and the rest are test groups, three in each group, are three a parallel sample. The total number of colonies and the content of pathogenic bacteria in the test group and the control group were measured respectively. Then, the plates were directly immersed in different concentrations of Bdellovibrio solutions. The test groups were soaked in the concentration of 10 1 pfu / mL, 10 2 pfu / mL, 10 ...
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