Application of bdellovibrio leech plastid in preventing and controlling bacteria in production process of raw steaks
A production process, Bdellovibrio technology, applied in the field of disinfection and sterilization, Bdellovibrio liquid, can solve the problems of incomplete cleaning, bacteria easy to infect bacteria, short shelf life, etc., to prevent bacterial infection, reduce the total amount of bacteria, and operate convenient effect
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Embodiment 1
[0041] The application of Bdellovibrio bdelloplasts in the prevention and control of the total number of colonies and pathogenic bacteria during the initial washing of fresh raw materials during the production of sashimi. The following sashimi takes sea bass as an example.
[0042] The application method includes the following specific steps and their conditions:
[0043] Step 1: Preparation of Bdellovibrio Liquid
[0044] The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a transparent circular phage plaque with a diameter of 2-3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. The fermentation method of hiruplasma is fermented: in 100mL MRS liquid medium (peptone 10g, beef extract powder 8g, yeast extract powder 4g, glucose 20g, dipotassium hydrogen phosphate 2g, triammonium citrate 2g, sodium acetate 5g, Magnesium sulfate 0.2g, manganese sulfate 0.05g, 1g Tween 80, pH value 6.0~6.4) conical flask inoculation 2mL concent...
Embodiment 2
[0059] The Bdellovibrio bdelloplasts are used in the disinfection and sterilization of sashimi production consoles and workshops.
[0060] The test consisted of the following specific steps and their conditions:
[0061] Step 1: Same as Step 1 of Example 1
[0062] Step 2: The Bdellovibrio bdelloplasty was applied to the disinfection and sterilization experiment of the sashimi production operation table and workshop.
[0063] Adopt the application method of the present invention, select the operating table and the processing workshop with the same size and specification for use, be divided into a plurality of different test groups and control groups, wherein three control groups are three parallel samples, and the rest are test groups, three in each group , for three parallel samples. Measure the total number of colonies and the pathogenic bacteria content of the test group and the control group respectively, then on the surface of the test group processing sashimi operation...
Embodiment 3
[0066] The Bdellovibrio solution is applied to the disinfection and sterilization of sashimi dishes.
[0067] The test consisted of the following specific steps and their conditions:
[0068] Step 1: Same as Step 1 of Example 1
[0069] Step 2: The application experiment of Bdellovibrio solution in the disinfection and sterilization of sashimi dishes.
[0070] Adopt the application method of the present invention, be divided into a plurality of different test groups and control groups, select the same dish of specification and size, wherein three control groups are three parallel samples, and the rest are test groups, three in each group, are three a parallel sample. The total number of colonies and the content of pathogenic bacteria in the test group and the control group were measured respectively. Then, the plates were directly immersed in different concentrations of Bdellovibrio solutions. The test groups were soaked in the concentration of 10 2 pfu / mL, 10 3 pfu / mL, ...
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