Serum free culture medium for growing various cells derived from kidney tissue

Abstract

The invention relates to a serum free culture medium for growing various cells derived from the kidney tissue. In the serum free culture medium, dulbecco's modified eagle medium (DMEM) / nutrient mixture F-12(F12)(1:1) is used as a basic culture medium and comprises the components including yeast autolysate, soy protein hydrolysate, amino acids, a fatty acid combining agent, an antioxidant reagent, reduced glutathione, a polyamine mixture, ethanolamine, beta-mercaptoethanol, glycerol, minerals, Pluronic F-68 and the like, and the pH value is 7.2. The culture medium of the invention can be used for culturing various cell lines derived from the kidney tissue, such as HEK293 cells, Marc145 cells, Vero cells and BHK21 cells and for propagating viruses.

Description

technical field [0001] The invention relates to a serum-free medium used for the growth of various kidney tissue-derived cells, especially capable of supporting the cultivation of HEK293 cells, Vero cells, Marc145 cells or BHK21 cells, and belongs to the field of cell engineering. Background technique [0002] The host cells currently used for the amplification of recombinant viral vectors, recombinant protein expression or large-scale preparation of vaccines are basically mammalian kidney tissue-derived cells, such as human embryonic kidney cells (HEK293), African green ape kidney cells (Vero), Rhesus monkey kidney cells (Marc145) and young hamster kidney cells (BHK21), etc. These cells usually need to add a certain amount (5-10%) of bovine serum in the traditional culture process. The serum contains growth factors, hormones, carrier proteins, adhesion factors, trace elements and other nutrients required for cell growth, which can Promotes cell growth. However, in large-s...

AI Technical Summary

Problems solved by technology

[0003] 1) The serum is susceptible to contamination by viruses, mycoplasma or other pathogens;

[0004] 2) Quality differences between different batches of serum cause quality differences between final product batches;

[0006] 4) It is difficult to completely remove some serum components, which seriously affects the quality of the final product and poses a safety hazard

[0009] 1) The serum-free medium still contains animal protein sources, such as bovine serum albumin (BSA), insulin, transferrin, etc., and there are still problems that may occur in the application of serum. In addition, it is expensive and generally only suitable for laboratories Use in small-scale cultivation;

[0010] 2) The currently developed serum-free medium has high specificity for cells, and usually a medium can only be suitable for the growth of one cell line, but not for other cell lines;

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