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Method for extracting purified aescine from horse chestnut

The technology of aescin and salsify is applied in the field of medicine, which can solve the problems of large usage of organic solvents, high fatty oil content, low extraction rate and the like, and achieves the effects of good impurity removal effect, less impurity and lower production cost.

Inactive Publication Date: 2011-02-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) Wang Xuying et al. "Extraction, Separation and Purification of Aescin in Borneo", the method disclosed in this document is to extract with 60% alcohol, extract with ethyl acetate and n-butanol successively, and vacuum-dry the n-butanol extract After concentration, apply NKA-9 resin and reversed-phase chromatography column. This method has a low extraction rate and a large amount of organic solvent, which is more suitable for the laboratory’s small-dose preparation requirements.
[0006] (2) Gao Xiaoning et al. "Research on Extraction Process of Aescin in Borneo", the method disclosed in this document is 95% ethanol immersion extraction, chloroform extraction after activated carbon decolorization, washing and drying, and the fatty oil content in the extract of this method is relatively high , low purity

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] Grind Polo Seeds, take 1kg, add 10 times the amount of ether to reflux for degreasing for 1 hour, filter, take the degreased medicinal residues, add 15 times the amount of 95% ethanol, and perform ultrasonic extraction for 30 minutes at a frequency of 40KHz and a temperature of 45°C. Repeat 3 times to extract After completion, centrifuge to obtain aescin extract, add 3g of activated carbon to the extract, reflux for decolorization for 1 hour, and suction filter to obtain a decolorization solution. The decolorization solution is filtered through a 0.2μm ceramic membrane, and then passed through Hollow composite membrane ultrafiltration, pressure 0.5Mpa, collect permeate, concentrate to 15% alcohol concentration, add 1L NKA-9 macroporous adsorption resin column for adsorption, first elute impurities with 5L water, flow rate is 40ml / min, and then Elute with 3L of 60% ethanol, control the flow rate to 50ml / min, collect the aescin fraction, concentrate the aescin fraction to ...

Embodiment 2

[0026] Grind spore seeds, take 1kg, add 8 times the amount of ether to reflux for defatting for 1 hour, filter, take the defatted medicinal residues, add 10 times the amount of 95% ethanol, and perform ultrasonic extraction for 50 minutes at a frequency of 60KHz and a temperature of 75°C, repeating twice to extract After completion, centrifuge to obtain aescin extract, add 5g of activated carbon to the extract, reflux for decolorization for 1.5h, and suction filter to obtain a decolorization solution, which is filtered through a 0.2μm ceramic membrane, and then passed through a molecular weight cut-off of 10000-6000 Hollow composite membrane ultrafiltration, pressure 1Mpa, collect permeate, concentrate to 20% alcohol concentration, add 1LNKA-2 macroporous adsorption resin column for adsorption, first elute impurities with 4L water, flow rate is 40ml / min, and then use Elute with 5L of 60% ethanol, control the flow rate to 40ml / min, collect the aescin fraction, concentrate the ae...

Embodiment 3

[0028] Grind Polo Seeds, take 2kg, add 10 times the amount of ether to reflux for defatting for 1 hour, filter, take the defatted medicinal residues, add 12 times the amount of 95% ethanol, and perform ultrasonic extraction for 1 hour at a frequency of 60KHz and a temperature of 40°C, repeat 3 times, extract After completion, centrifuge to obtain aescin extract, add 8g of activated carbon to the extract, reflux for decolorization for 1 hour, and suction filter to obtain a decolorization solution. The decolorization solution is filtered through a 0.2μm ceramic membrane, and then passed through Hollow composite membrane ultrafiltration, pressure 0.6Mpa, collect permeate, concentrate to alcohol concentration of 18%, add 1L X-5 macroporous adsorption resin column for adsorption, first elute impurities with 7L water, flow rate is 50ml / min, and then Elute with 3L of 60% ethanol, control the flow rate to 60ml / min, collect the aescin fraction, concentrate the aescin fraction to 1 / 8 of ...

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Abstract

The invention discloses a method for extracting purified aescine from horse chestnut, which comprises the following steps: carrying out reflow degreasing on raw materials by adopting diethyl ether, then putting the obtained dregs of a decoction in an extractor; adding ethanol into the extractor to carry out ultrasonic extraction; after the ultrasonic extraction is completed, carrying out centrifugal separation on the obtained product so as to obtain aescine extracting solution; carrying out decoloration on the aescine extracting solution by active carbons; carrying out microfiltration and ultrafiltration on the aescine extracting solution subjected to decoloration; concentrating the obtained filter liquor until the concentration of the filter liquor is lower than that of ethanol; carrying out adsorption on the obtained filte with water and alcohol; collecting aescine fractions and recycling reagent; and standing the aescine fractions for crystallization and carrying out recrystallization on the obtained product by acetone and aether. The method is suitable for scale-up production.

Description

Technical field: [0001] The invention belongs to the technical field of medicines, and relates to a method for extracting and purifying aescin from baria. Background technique: [0002] Borneo is the dry mature seed of Aesculus chinensis Bge., Aesculus chinensis Bge.var.ch ekiangensis (Hu et Fang) Fang or Aesculus wilsonii Rehd. It has the effects of regulating qi, widening the middle, harmonizing the stomach and relieving pain, and is used to treat chest tightness, distending pain and epigastric pain. [0003] Aescin is the active ingredient of Borneo, which belongs to triterpenoid saponin compound. It has anti-inflammatory, anti-exudation, restores capillary permeability, improves venous tension, improves blood circulation, expands arteries, increases hypoxia resistance, and promotes brain function. function restoration. Compared with sodium aescinate, aescin or aescin has weaker side effects on the human body, but has no effect on the therapeutic effect on the human bod...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H1/08C07H15/256
Inventor 刘东锋万冬梅杨成东
Owner NANJING ZELANG MEDICAL TECH
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