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Method for identifying fx151 genetic variation map by using litopenaeus vannamei boone fx151 microsatellite DNA marker

A fx151, DNA marker technology, applied in the field of identifying genetic variation maps, can solve problems such as applications that have not yet been reported, and achieve the effect of a simple method

Inactive Publication Date: 2011-02-09
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Before the present invention was made, there were similar research reports at home and abroad, such as "Screening of Microsatellite DNA in Chinese Prawns" published by Xu Peng et al. Shrimp Molecular Marker Screening, Linkage Map Construction and QTL Mapping Foreign Franklin Pérez et al. (2005) found 112 pairs of microsatellites in Litopenaeus vannamei. Applications such as specific genetic markers have not been reported

Method used

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  • Method for identifying fx151 genetic variation map by using litopenaeus vannamei boone fx151 microsatellite DNA marker
  • Method for identifying fx151 genetic variation map by using litopenaeus vannamei boone fx151 microsatellite DNA marker

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Embodiment

[0019] First, extract the genome of Litopenaeus vannamei and dilute it for later use, and then use the core sequence of Litopenaeus vannamei fx151 microsatellite DNA to design specific primers at both ends of the sequence; then use the primers to analyze different populations of Litopenaeus vannamei or individuals within the population. The genomic DNA is amplified by PCR, and the PCR products are detected by denaturing polyacrylamide gel; the silver-stained bands of the products are used for analysis to determine the genotype of each individual, so as to obtain the inheritance of Litopenaeus vannamei in fx151 Polymorphism map of marker gene locus (such as figure 1 Shown).

[0020] 1 Extract the genome of Litopenaeus vannamei

[0021] 1) Take 30 mg of Litopenaeus vannamei muscle into a 2.0 ml centrifuge tube, cut into pieces, and add 600 ul STE lysis buffer (100 mmol / L NaCl, 10 mmol / L Tris-Cl, pH 8.0; 1 mmol / L) L EDTA, pH 8.0), 50 μl SDS and 5 μl proteinase K (20mg / ml), mix well...

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Abstract

The invention discloses a method for identifying an fx151 genetic variation map by using a litopenaeus vannamei boone fx151 microsatellite Deoxyribonucleic Acid (DNA) marker, which is characterized by comprising the following steps of: extracting a litopenaeus vannamei boone genome and diluting for later use, designing specific primers at two ends of a sequence by utilizing a litopenaeus vannamei boone fx151 microsatellite DNA core sequence, performing Polymerase Chain Reaction (PCR) amplification on genomes DNA of different groups or individuals in the groups of the litopenaeus vannamei boone by using the primers, and performing denaturing polyacrylamide gel detection on a PCR product; and performing silver staining development by utilizing stripes generated by the product and analyzing, and determining a genotype of each individual to obtain a polymorphic genetic variation map of the litopenaeus vannamei boone at a fx151 genetic marker gene locus. The method can rapidly obtain the polymorphic map of the litopenaeus vannamei boone fx151 genetic marker gene locus which presents high genetic variation, is convenient and simple, and the obtain result can intuitively detect the genotype of each individual litopenaeus vannamei boone.

Description

Technical field [0001] The present invention belongs to the field of molecular biology DNA marking technology and application, and specifically relates to marine economic animal Penaeus vannamei ( Litopenaeus vannamei ) The method of identifying genetic variation map of fx151 microsatellite DNA marker. Background technique [0002] Litopenaeus vannamei ( Litopenaeus vannamei ), commonly known as Penaeus vannamei ( Penaeus vannamei ), belongs to the arthropod phylum ( Arthropoda ), Crustacea ( Crustacea ), decapoda ( Decapoda ), swimming suborder ( Natantia ), Penaeidae ( Penaeidae ), Penaeus ( Penaeus ), Lito-penaeus The subgenus is a wide-temperate and broad-salt tropical shrimp. It is currently the world’s three largest shrimp species (Penaeus chinensis, Penaeus monodon, Penaeus vannamei) with the highest yield per shrimp species. It occupies the shrimp fishery and aquaculture industry. important position. [0003] In July 1988, Litopenaeus vannamei was introduced to my c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 吕玲李朝政翁少萍何建国
Owner SUN YAT SEN UNIV
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