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Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and adrenal gland

A technology of lung adenocarcinoma cell lines and metastatic potential, which is applied in the field of Chinese lung adenocarcinoma cell lines, can solve the problems of limited collection of clinical specimens, delay in the research progress of diagnosis and treatment of bone metastasis of lung cancer, etc., and achieve complete cell morphology and good uniformity and reproducible, metabolically vigorous effects

Inactive Publication Date: 2011-01-26
SHANGHAI CHEST HOSPITAL
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Problems solved by technology

[0004] Since the occurrence of lung cancer bone metastases is mostly seen in patients with advanced surgical contraindications, the clinical specimen collection is limited, which largely delays the progress of research on the diagnosis and treatment of lung cancer bone metastases.

Method used

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  • Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and adrenal gland
  • Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and adrenal gland
  • Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and adrenal gland

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Take the pleural effusion of a first-diagnosed patient with lung adenocarcinoma (a 55-year-old female) for primary cell culture, collect 50 ml of fresh light yellow pleural effusion, add 8 ml of pleural effusion to each cell culture bottle, add 2 ml of RPMI1640 culture medium without bovine serum and double antibody After that, shake them evenly, put them into a German Heraeus BB16 carbon dioxide incubator with a culture temperature of 37°C and a CO2 concentration of 5% for cultivation. Five days later, when observed under a light microscope, it was found that about 30% of the area at the bottom of the cell culture flask was covered with cells. The cells grow adherently; most of the adherent cells are polygonal. Cells are larger. The cells grow slowly at the initial stage of culture; the cell shape is stable, and the cell shape and growth speed become slightly faster at the 10th passage. The properties remained unchanged after cryopreservation and recovery. Observe t...

Embodiment 2

[0044] The above-mentioned human lung adenocarcinoma cells (CPA-Yang3) 70×10 4 , injected into the blood system of immunodeficient mice, weighed every other day after two weeks, and carried out radionuclide bone imaging detection every week from the third week, the results confirmed that the parental cells were lung cancer cells with high potential bone metastases (Figure 8).

[0045] The medium is RPMI1640 or DMEM medium containing 10% fetal bovine serum, pH=7, and the culture temperature is 37° C., 5% CO2.

Embodiment 3

[0047] RT-PCR detection showed that compared with the SPC-A-1 Chinese lung adenocarcinoma established in the prior art, the gene expression levels of some oncogenes ESM1, VEGF-C, IL-6, IL-8, SVIL, AR and FN1 significantly higher than SPC-A-1 cells.

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Abstract

The invention belongs to the filed of microorganism animal cell lines and provides a Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and adrenal gland. According to the invention, hydrothorax of an adenocarcinoma first-diagnosis patient is used as primary culture of cells; the human adenocarcinoma cell has the preservation number of CGMCC No.3137 and classified name as human lung adenocarcinoma cell line CPA-Yang3, can grow adhered to the wall and has the tumorigenesis rate up to 100 percent; the human lung adenocarcinoma cell can grow quickly and metabolize vigorously; the expression levels of cancer genes EMS1, VEGF-C, IL-6, IL-8, SVIL and AR gene are higher than the expression level of SPC-A-1 cell; and the lung adenocarcinoma cancel cell has the biological characteristics of mainly transferring the bone and having high transfer potential of the lever and the adrenal gland, high growth speed of bone transfer cells and complete cell morphology. The invention can be used for providing reference data to early diagnosis for transferring the human lung adenocarcinoma bone, further establishing the relevant gene chip technology and assessing and exploring the curative effects of various medicaments and the like by comprehensively applying the gene chip, quantifying PCR (Polymerase Chain Reaction), western blot in real time and other technologies.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, and specifically relates to a Chinese lung adenocarcinoma cell line with high potential for bone, liver and adrenal gland metastasis and its application. Background technique [0002] Lung cancer is one of the malignant diseases that seriously threaten the survival and development of human beings, and has become the number one killer of tumor diseases. In 2006, about 174,470 new cases of lung cancer were diagnosed in the United States, accounting for about 13% (male) and 12% (female) of all tumors. 31% (men) and 26% (women), the highest death rate [1～2] . There are more than 50,000 new cases of lung cancer in Japan every year, and the mortality rate is nearly 90%. [3] . According to the incidence survey of malignant tumors in Shanghai in recent years, lung cancer (21.56%) ranks first in the incidence of malignant tumors in Shanghai males, and it is second only to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12Q1/68C12Q1/18C12R1/91
Inventor 杨顺芳
Owner SHANGHAI CHEST HOSPITAL
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