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Method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein

A blood pressure-lowering peptide and mussel technology, applied in the field of bioengineering, can solve the problems of low activity of the blood pressure-lowering peptide ACE inhibitory peptide, etc., and achieve the effects of strong industrial implementation, ACE inhibition and low price.

Active Publication Date: 2011-01-19
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently known methods for preparing ACE inhibitory peptides from clams and oysters are not suitable for preparing hypotensive peptides from mussels (the reason is that the activity of the obtained ACE inhibitory peptides is low)

Method used

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  • Method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein
  • Method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein
  • Method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. A method for preparing antihypertensive peptides by enzymatically hydrolyzing mussel protein, followed by the following steps:

[0044] 1). Prepare defatted dried mussel powder:

[0045] After the fresh blue mussels are cleaned with water, the shells are removed and the shreds are removed. After being homogenized by a beater to a paste, the fresh blue mussels are freeze-dried (-50°C, 72 hours) to obtain mussel powder. Add the mussel powder to a 95% volume concentration ethanol solution at a ratio of 1g:4ml at 40℃ to defat for 4 hours, repeat three times; vacuum dry the mussel powder after the above degreasing treatment at 40℃ (5 hours), crush And pass a 100-mesh sieve to obtain defatted dried mussel powder with a moisture content of ≤2.34%.

[0046] 2) Weigh 5.0g of the above-mentioned defatted dried mussel powder (protein weight content 60.23%) into the enzyme reactor, add 100ml deionized water and stir evenly, homogenize for 10min, adjust the pH to 9.2 with 0.5mo...

Embodiment 2

[0058] Example 2. A method for preparing antihypertensive peptides by enzymatically hydrolyzing mussel protein, followed by the following steps:

[0059] 1). Prepare defatted dried mussel powder:

[0060] Replace fresh purple mussel with fresh thick-shell mussel, and the rest are the same as in Example 1.

[0061] 2) Weigh 5.0g of defatted dried mussel powder (protein weight content 72.56%) in the enzyme reactor, add 100ml deionized water and stir evenly, homogenize for 10min, adjust the pH to 9.2 with 0.5mol / L NaOH solution; Get the mixture.

[0062] 3). Raise the temperature of the mixture to 60℃ and stir to preheat, and keep it under stirring for 20 minutes; then add 0.075g of alkaline protease (Alcalase, enzyme activity of 9.4×10 4 U / g) Start the enzymatic hydrolysis reaction, the enzymatic hydrolysis temperature is 60-65°C, continuous stirring, keep the temperature and pH of the reaction system constant, and the enzymatic hydrolysis time is 240 minutes. Get the product of enzyma...

Embodiment 3

[0073] Example 3. A method for preparing antihypertensive peptides by enzymatically hydrolyzing mussel protein, followed by the following steps:

[0074] 1). Prepare defatted dried mussel powder:

[0075] The same as in Example 1.

[0076] 2) Weigh 200.0g of defatted dried mussel powder (protein weight content 60.23%) in the enzyme reactor, add 5L deionized water and stir evenly, homogenize for 10min, adjust the pH to 9.3 with 0.5mol / L NaOH solution; Get the mixture.

[0077] 3). Raise the temperature of the mixed solution to 65℃ and stir to preheat, and keep it under stirring for 20 minutes; then add 2.0g of alkaline protease (Alcalase, enzyme activity of 9.4×10 4 U / g) Start the enzymatic hydrolysis reaction, the enzymatic hydrolysis temperature is 65°C, continuous stirring, keep the temperature and pH of the reaction system constant, and the enzymatic hydrolysis time is 180 minutes. Get the product of enzymatic hydrolysis.

[0078] 4). The enzymatic hydrolysate obtained in step 3) i...

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Abstract

The invention discloses a method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein, which comprises the following steps: 1) mixing defatted dry mussel powder or mussel protein powder serving as raw material with water, then adjusting the pH value to 9.0 to 9.5 so as to obtain a mixed liquor; 2) raising the temperature of the mixed liquor to 60 to 65 DEG C for preheating, then adding alkali protease into the mixed liquor to carry out zymolysing reaction, wherein the zymolysing temperature is 60 to 65 DEG C and the zymolysing time is 180 to 240 minutes; and 3) carrying out enzyme inactivation processing on zymolysing products obtained in step 2 firstly so as to obtain zymolysing liquid; then sequentially carrying out ultra-filtration, freeze drying and chromatography on the obtained zymolysing liquid so as to obtain the ACEIP. The ACEIP prepared by using the method of the invention has the characteristic of strong activity.

Description

Technical field [0001] The invention belongs to the field of bioengineering technology, and specifically relates to biopharmaceutical technology or functional food processing technology, in particular to a method for preparing antihypertensive peptides by enzymatically hydrolyzing mussel protein. Background technique [0002] Angiotensin-converting enzyme (ACE) plays an important role in the regulation of human blood pressure: excessively high ACE activity in human circulation or local tissues will increase the production of angiotensin II, and at the same time, relieve the amount of bradykinin Greatly reduced, directly leading to increased blood pressure. Therefore, by inhibiting the activity of ACE in the body, blood pressure can be lowered. [0003] At present, ACE inhibitors have become the first-line drugs for the treatment of hypertension. Compared with synthetic ACE inhibitors, ACE inhibitory peptides obtained by enzymatic hydrolysis of food-borne proteins have many advanta...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
Inventor 戴志远张艳萍张婷张燕平
Owner ZHEJIANG GONGSHANG UNIVERSITY
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