Method for screening methionine resistant deinsectization streptomyces avermitilis strain

A technology for exterminating Streptomyces sp. and methionine, which is applied in biochemical equipment and methods, treatment of microorganisms with electricity/wave energy, and enzyme treatment with electricity/wave energy, etc. It can solve problems such as large workload and lack of orientation.

Active Publication Date: 2011-01-19
XINYU PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a screening method for methionine-resistant Streptomyces avermitilis strains, which solves the problems of large workload and lack of orientation in the existing strain selection

Method used

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  • Method for screening methionine resistant deinsectization streptomyces avermitilis strain
  • Method for screening methionine resistant deinsectization streptomyces avermitilis strain
  • Method for screening methionine resistant deinsectization streptomyces avermitilis strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1), take the slope of the grown Streptomyces avermitilis strain AV-4 as the starting strain, add an appropriate amount of purified water inside, gently scrape off the spores to obtain the spore liquid, and suck the spore liquid into a container In the Erlenmeyer flask of a small amount of glass beads, vibrate for 10-15min to break up the spore chain and filter to obtain Streptomyces avermitilis monospore suspension 1;

[0030] (2), placing the Streptomyces avermitilis monospore suspension under a 30W ultraviolet lamp and irradiating it for 30S, inserting the spore liquid into a liquid medium containing 3% methionine, at a temperature of 27.5-28.5°C and a humidity of 30- Cultured for 6 days under the condition of 50%, enriching the strains tolerant to 3% methionine;

[0031] (3), insert the enriched 3% methionine-tolerant bacterial strain into the slant medium, cultivate it for 9 days at a temperature of 27.5-28.5°C and a humidity of 30-50%, and then take it from the sl...

Embodiment 2

[0055] The same procedure as in Example 1 was adopted, except that the starting strain AV-4 was replaced by AV-21.

[0056] Example 2 The shake flask fermentation capacity of 46 single-spore slant was investigated. Taking the starting strain AV-21 as the control, the results are shown in Table 4 below, 6 of the 46 strains were higher than the control, the highest titer was 5058u / ml, and the increase rate was 10.2%.

[0057] Table 4 Shake flask primary screening results of methionine-resistant strains

[0058]

[0059] The 6 strains with higher titers in the primary screening were stored for strain preservation, and at the same time they were inoculated into the slant medium for re-screening. The results of the re-screening are shown in Table 5 below, and 3 of the 6 strains were higher than the control.

[0060] Table 5 methionine-resistant strain shake flask re-screening results

[0061]

[0062] AVm2-3, AVm2-4, AVm2-22 and the starting strain were carried out on a sla...

Embodiment 3

[0066] The same steps as in Example 1 were adopted, except that the starting strain AV-4 was replaced by AV-36.

[0067] Example 3 The shake flask fermentation capacity of 46 single-spore slant was investigated. Taking the starting strain AV-36 as the control, the results are shown in Table 7 below, 3 of the 46 strains were higher than the control, the highest titer was 5348u / ml, and the increase rate was 12.3%.

[0068] Table 7 Shake flask primary screening results of methionine-resistant strains

[0069]

[0070] The 3 strains with higher titers in the primary screening were preserved, and inoculated into the slant medium for re-screening. The results of the re-screening are shown in Table 8 below, and one of the 3 strains is higher than the control.

[0071] Table 8 methionine-resistant strain shake flask re-screening results

[0072]

[0073] AVm3-6 and the starting strain were carried out on a slant, and the obtained slant of each offspring and the slant of the co...

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Abstract

The invention discloses a method for screening a methionine resistant deinsectization streptomyces avermitilis strain, which belongs to the field of the screening of microbial strains. The method comprises the following steps of: performing ultraviolet mutagenesis on deinsectization streptomyces avermitilis spore suspension; performing enrichment on tolerant strains by using a methionine-containing liquid culture medium; screening methionine solid-plate tolerant strains; and screening strains of which the shake flask capacity is improved compared with that of parent strains through preliminary screening, secondary screening and genetic stability research so as to provide a feasible method for screening the deinsectization streptomyces avermitilis strain. The shake flask production capacity of the deinsectization streptomyces avermitilis strain screened by the method is greatly improved, and the genetic stability is high.

Description

technical field [0001] The invention relates to the technical field of microbial strain screening, in particular to a method for screening methionine-resistant Streptomyces avermitilis strains. Background technique [0002] Avermectins (AVM) is a sixteen-membered ring lactone multi-component antibiotic produced by Streptomyces avermitilis, according to the three positions of C-5, C22-23 and C26 in its molecule Structural differences can be divided into A1a, A1b, A2a, A2b, B1a, B1b, B2a, B2b 8 kinds of components. Among them, B1 component, especially B1a, has the highest anti-nematode and arthropod activity, and is widely used in the treatment of livestock parasite infection and agricultural pest control, and has become an important antibiotic in agriculture and animal husbandry. Abamectin is divided into 8 active components, only B 1a and B 1b Can be used as medicine, especially B 1a The highest activity. In the synthetic metabolism of abamectin, the C5-oxymethylase cat...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N15/01
Inventor 杨春丽娄敏李为全刘瑞华
Owner XINYU PHARM CO LTD
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