Method for quickly detecting colibacillus and used micro flow control chip as well as preparation technique

Abstract

The invention discloses a method for quickly detecting colibacillus, comprising the following steps: firstly, using anti-goat colibacillus antibody to mark functional fluorescent nanometre particle, then, adding to a sample to be detected, introducing the sample to be detected to a micro flow control chip, using a signal supply system to supply the corresponding frequency and voltage in the chip,observing and recording a fluorescent signal nearby an electrode at certain flow speed, at last, judging whether the sample to be detected has the colibacillus and judging the concentration. The usedmicro flow control chip comprises a base sheet and a cover sheet which are adhered together; a capillary passage is assembled between the base sheet and the cover sheet; the two ends of the passage respectively are respectively connected with a sample inlet and a sample outlet on the cover sheet; and the passage is internally provided with paired platinum electrodes. The method has the advantagesof good specificity, high flexibility, low cost, quick speed, strong selectivity and suitability.

Description

technical field [0001] The invention relates to a detection technology of pathogenic bacteria, in particular to a detection method of Escherichia coli, a detection chip and a preparation method thereof. Background technique [0002] In the development of human society, diseases have always played the role of killers that pose the greatest threat to human survival and reproduction. The diseases caused by the pollution of food, water, ambient air and soil by various pathogenic bacteria seriously endanger human health. At present, the detection methods of common pathogenic bacteria have the disadvantages of long detection period, heavy workload, many reagents required, high false negative rate, low sensitivity, or high false positive rate and poor specificity. Therefore, the establishment of fast, simple, accurate and specific detection methods for pathogenic bacteria can be used for the prevention, early diagnosis and correct treatment of human infectious diseases, the effect...

AI Technical Summary

Problems solved by technology

However, the traditional culture method also has serious deficiencies: (1) The method of bacterial culture is cumbersome and time-consuming (generally takes days to weeks), and it cannot detect multiple pathogenic bacteria at the same time; (2) The concentration of pathogenic bacteria often Low, there will be errors in sampling and counting; (3) It is not suitable for detecting some pathogenic bacteria that are difficult or impossible to culture artificially

However, with the increase in the complexity of detection samples and the urgency of detection tasks, the existing sensing technologies for pathogen detection are still facing severe challenges.

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