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Active site composition of isatis roots, as well as preparation method and application thereof

A technology of active parts and Radix isatidis extract, applied in the field of medicine, can solve the problems of not very high content of active parts, inconsistent extraction and purification methods, etc., and achieve the effects of good synergy, obvious antiviral activity, and strong operability.

Inactive Publication Date: 2011-01-05
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, quite a few people have studied the parts of Radix Isatidis, including alkaloids, organic acids, etc., but the content of the effective parts is not very high, and the extraction and purification methods are inconsistent, especially when there is no combination of active parts for efficacy. Applied coverage

Method used

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  • Active site composition of isatis roots, as well as preparation method and application thereof
  • Active site composition of isatis roots, as well as preparation method and application thereof
  • Active site composition of isatis roots, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Radix Isatidis active part composition

[0033] (1) Take 1Kg of Radix isatidis, extract with 8 times and 6 times of water for 2h and 1.5h respectively under reflux, combine the extracts, let cool, filter, recover and concentrate under reduced pressure to obtain the water-extracted part, add 95% to the extract Ethanol, until the final concentration of ethanol is 70%, let stand overnight, filter and filter to obtain the precipitate and supernatant. The precipitate is dried to obtain 45g of Radix isatidis total polysaccharides. The total polysaccharide content is 65.16% as measured by the phenol-sulfuric acid method; the supernatant D101 macroporous adsorption resin, first eluted with water, and then eluted with a 10%-60% alcohol solution, collected the alcohol eluate, concentrated and dried, to obtain 15g of Radix isatidis total phenylpropanoids, the total was measured by UV spectrophotometry The content of phenylpropanoid is 64.4%;

[0034] (2) Take ...

Embodiment 2

[0037] Example 2 Preparation of Radix Isatidis active part composition

[0038] Steps (1) and (2) are the same as in Example 1. Take 30 parts of Radix Isatidis, 40 parts of total organic acids and 20 parts of total alkaloids prepared in steps (1) and (2), and mix well Available (batch number 002).

[0039] Take 20g of the above Radix isatidis active part composition, add 10g of medicinal starch, mix evenly, add water to make soft material, pass through a 20-mesh sieve, granulate, dry, make granules, put into capsules, 0.2g each, or take Radix isatidis active The part composition is 10g, corn starch is 5g, the lubricant magnesium stearate 5g is added, mixed evenly, and then compressed into tablets. Or take 10 g of the above Radix isatidis active part composition, add 8 g of medicinal starch, mix uniformly, sizing through a 20-mesh sieve, and dry to prepare granules.

Embodiment 3

[0040] Example 3 Preparation of Radix isatidis active part composition

[0041] (1) Take 1Kg of Radix Isatidis and extract 2h and 1.5h ultrasonically with 8 times and 6 times the amount of water respectively, combine the extracts, let cool, filter, recover and concentrate under reduced pressure to obtain the water-extracted part, filter with ultrafiltration membrane, and intercept The filtrate with a molecular weight of 1,000 to 1,000,000 is dried to obtain 48 g of Radix isatidis total polysaccharide content. The total polysaccharide content is 60.21% as measured by the phenol-sulfuric acid method; 95% ethanol is added to the extract to reach a final ethanol concentration of 80%. Let stand overnight and filter , Filter the precipitate and supernatant, the supernatant on the D101 macroporous adsorption resin, first eluted with water, and then with a concentration of 10% to 60% alcohol solution, collect the alcohol eluate, concentrate and dry, get the isatis root total benzene 17g ...

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Abstract

The invention discloses an active site composition of isatis roots, as well as a preparation method and the application thereof. The active site composition of isatis roots is prepared from a general phenylpropanoid part of isatis roots, a total alkaloid part, a total organic acid part and a total polysaccharide part. The active site composition obtained by the invention has high effective component contents and accurate active components. Proved by experimental results, the active site composition of isatis roots has obvious antiviral activity and favorable antibacterial and antipyretic activities. After being combined, the effective components reach favorable synergy, exert the antiviral, antibacterial and antipyretic activities at a plurality of targets spots through multiple paths, can be used for treating a viral disease with the bacterial inflammation of a febrile disease and have a wider clinical application range. Moreover, the invention optimizes and designs the extracting and refining process according to the physicochemical properties of the effective components, the active parts obtained through preparation has high effective component contents, and the preparation method has strong maneuverability and can realize industrialized mass production.

Description

Technical field [0001] The invention relates to a Radix isatidis medicinal preparation, in particular to a Radix isatidis active part composition with antiviral, antibacterial and antipyretic activity and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Isatis root has the functions of clearing away heat, detoxifying, cooling blood and relieving throat. It is often used in modern clinical practice for viral diseases and bacterial infections, especially in antiviral effects. Many literatures reported that Isatis root is effective for influenza caused by viruses, hepatitis B, herpes simplex, Viral myocarditis, hemorrhagic fever with nephrotic syndrome and other diseases have good clinical treatment or prevention effects. As one of the commonly used traditional Chinese medicines for clearing away heat and detoxification, Isatis indigotica is embodied in modern pharmacology as anti-virus, immune enhancement and anti-oxidant syn...

Claims

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Application Information

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IPC IPC(8): A61K36/315A61P31/12A61P31/04A61P29/00A61K125/00
Inventor 李祥陈建伟陈妍王盛
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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