Method for detecting single nucleotide polymorphism of cattle chemerin gene

Abstract

The invention discloses a method for detecting single nucleotide polymorphism of a cattle chemerin gene. Gene polymorphism comprises A or G base polymorphism in the 868th site of the cattle chemerin gene and G or A base polymorphism in the 1021st site of the cattle chemerin gene. The detection method for the single nucleotide polymorphism of the cattle chemerin gene comprises the following steps of: by using a cattle entire genome DNA (deoxyribonucleic acid) to be detected, containing a chemerin gene, as a template, and a primer pair (comprising P and newP) as primers, carrying out PCR (Polymerase Chain Reaction) amplification on the cattle chemerin gene; after a PCR amplified product is digested by using restriction enzyme Pvu II, carrying out agarose gel electrophoresis on an amplified fragment subjected to enzyme digestion; and identifying the single nucleotide polymorphism of the 868th site and the 1021st site of the cattle chemerin gene according to an agarose gel electrophoresis result. The method is used for screening and detecting a molecular genetic marker which is closely related to cattle production trait on the basis of a DNA level, carrying out marker assisted selection (MAS) on Chinese cattle beef growth trait and quickly establishing cattle specie groups with favorable genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the 868th and 1021st SNPs of cattle chemerin gene. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Therefore, commonly referred to as SNPs include base substitutions, insertions, deletions, and changes in the copy number of repeated sequences. A SNP indicates that there is a nucleotide change at a certain position in the genome, which is mainly caused by the conversion or transversion of a single base; SNPs with conversion mutations account for about 2 / 3, and other SNPs are in similar level. The cytosine of the CpG dinucleotide is the most mutated site in the genome, most of which are methylated and can be spontaneously deaminated to form thymine. [...

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Problems solved by technology

There is only one study on genetic variation of chemerin gene in humans (Mussig K, StaigerH, Machicao F, Thamer C, Machann J, Schick F, Claussen CD, Stefan N, FritscheA, Haring HU. RARRES2, encoding the novel adipokine chemerin, is a genetic determinant of disproportionate regional body fat distribution: A comparative magnetic resonance imaging study. Metabolism. 2009; 58: 519-524.), but so far there has been no report on the genetic variation or SNP study of the cattle chemerin gene

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