Radial flow chromatographic separation and purification method for biological polysaccharide
A chromatographic separation and purification method technology, applied in the field of separation and purification of biological polysaccharides using macroporous resin radial flow chromatography technology, can solve the problems of complex separation and purification process, adverse effects of drug quality, operator and environmental hazards, and achieve simple process , shorten the separation and purification time, and facilitate the operation
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Embodiment 1
[0016] A macroporous resin radial flow chromatography for directly separating and purifying exopolysaccharides from the fermentation supernatant of medicinal fungi, first centrifuging the liquid fermentation product of medicinal fungi at 4000 rpm for 10 minutes, collecting the supernatant, and then , the fermentation supernatant containing fungal polysaccharides is directly loaded on a 500 ml macroporous resin radial chromatographic column at a flow rate of 60 ml / min. Flow rate is 50 milliliters / minute, collects 530 milliliters of eluents, is the polysaccharide solution that contains purification, through measuring with phenol-sulfuric acid method, the recovery rate of polysaccharide is 85.2%, through spectrophotometry, the pigment content in the reclaimed polysaccharide The reduction can reach 82.3%. As determined by the Bradford method, the protein content in the recovered polysaccharide can be reduced by up to 85.6%, so that both the recovery rate and the purity of the polys...
Embodiment 2
[0018] A macroporous resin radial flow chromatography for directly separating and purifying intracellular polysaccharides from the supernatant of mycelium enzymolysis. First, the mycelium enzymolysis product is centrifuged at 6000 rpm for 10 minutes to collect the supernatant, and then, The enzymolysis supernatant containing fungal polysaccharides is directly loaded on a 500 ml macroporous resin radial chromatographic column at a flow rate of 120 ml / min. 200 milliliters / min, collect 800 milliliters of eluents, namely contain the polysaccharide solution of purification, through measuring with phenol-sulfuric acid method, the recovery rate of polysaccharide is 98.1%, measure through spectrophotometry, the pigment content reduction in reclaiming polysaccharide can be As determined by the Bradford method, the protein content in the recovered polysaccharide can be reduced by up to 75.6%, so that the recovery rate and purity of the polysaccharide are significantly increased.
Embodiment 3
[0020] A macroporous resin radial flow chromatography for directly separating and purifying polysaccharides from fungal fermentation supernatants. First, the fungal liquid fermentation product is centrifuged at 5000 rpm for 10 minutes to collect the supernatant. The fermentation supernatant is directly loaded on a 500 ml macroporous resin radial chromatographic column at a flow rate of 90 ml / min, with a sample volume of 500 ml, and then eluted with distilled water at a flow rate of 100 ml / min , collect 650 milliliters of eluents, namely contain the polysaccharide solution of purification, through measuring with phenol-sulfuric acid method, the recovery rate of polysaccharide is 94.9%, measure through spectrophotometry, the pigment content in reclaiming polysaccharide reduces and can reach 79.2%, As determined by the Bradford method, the protein content in the recovered polysaccharide can be reduced by up to 82.6%, so that both the recovery rate and the purity of the polysacchar...
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