Horsebean biological compound fertilizer prepared from ardealite, preparation method thereof and use thereof
A technology of biological compound fertilizer and phosphogypsum, which is applied in the field of agriculture, can solve the problems of production and application restrictions, destruction of ecological balance, slow growth of broad beans, etc., and achieve the effect of inhibiting the invasion of pathogenic microorganisms, inhibiting harmful bacteria, and protecting the growth of broad beans
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Embodiment 1
[0024] (1) Bacteria production:
[0025] 1) Rhizobium faba: the liquid medium consists of yeast extract 0.05-0.3% (weight / volume), mannitol 0.5-2.0% (volume / volume), 0.05-0.3% magnesium sulfate (weight / volume), 0.1-0.3 % ferric chloride (weight / volume), 0.1-0.3% sodium molybdate (weight / volume), 0.01-0.15% zinc sulfate (weight / volume), 0.08-0.25% potassium dihydrogen phosphate (weight / volume), 0.1-0.2% calcium chloride (weight / volume), pH 6.6-7.2, sterilized at 121°C for 25-40 minutes. Cool to about 35°C, inoculate broad bean rhizobia, the inoculation amount is about 2-5×10 per milliliter of liquid medium 6 A broad bean rhizobia was cultured on a shaking table with a rotating speed of 140-180 rpm, and after 48-72 hours, the number of rhizobia in the fermentation liquid reached 20-30×10 per milliliter of liquid. 8 hour, the fermentation is over;
[0026] 2) Glomus: mix peat, fine river sand, field fine soil, and phosphogypsum powder according to the ratio of (3-8):(2-5):(1-2...
Embodiment 2
[0031] (1) Bacteria production:
[0032] 1) Broad bean rhizobia liquid 1000mL: The liquid medium is composed of 1.5g of yeast extract, 1.8g of mannitol, 0.8g of magnesium sulfate, 1.8g of ferric chloride, 2.5g of sodium molybdate, 0.5g of zinc sulfate, and potassium dihydrogen phosphate 1g, calcium chloride 2g, pH6.6-7.2, sterilized at 121°C for 25-40 minutes. Cool to about 35°C, inoculate with Rhizobium fabae, the inoculation amount is about 5x10 per milliliter of liquid medium 6 broad bean rhizobia, cultivated on a shaking table with a rotating speed of 100-180 rpm, and after 48-72 hours, the number of rhizobia in the fermentation liquid reached 20-30×10 per milliliter of liquid 8 1 hour, fermentation finishes, obtains 1000mL of broad bean rhizobium fermented liquid;
[0033] 2) Glomus preparation 1000mL: Mix 500g of peat, 300g of fine river sand, 199.7g of field fine soil, and 0.3g of phosphogypsum powder, grind and screen the particles below 3 mm, and heat-preserve and s...
Embodiment 3
[0037] (1) Bacteria production:
[0038] 1) Rhizobium faba: the liquid medium consists of yeast extract 0.05-0.3% (weight / volume), mannitol 0.5-2.0% (volume / volume), 0.05-0.3% magnesium sulfate (weight / volume), 0.1-0.3 % ferric chloride (weight / volume), 0.1-0.3% sodium molybdate (weight / volume), 0.01-0.15% zinc sulfate (weight / volume), 0.08-0.25% potassium dihydrogen phosphate (weight / volume), 0.1-0.2% calcium chloride (weight / volume), pH 6.6-7.2, sterilized at 121°C for 25-40 minutes. Cool to about 35°C, inoculate broad bean rhizobia, the inoculation amount is about 2-5×10 per milliliter of liquid medium 6 A broad bean rhizobia was cultured on a shaking table with a rotating speed of 140-180 rpm, and after 48-72 hours, the number of rhizobia in the fermentation liquid reached 20-30×10 per milliliter of liquid. 8 hour, the fermentation is over;
[0039] 2) Glomus: mix peat, fine river sand, field fine soil, and phosphogypsum powder according to the ratio of (3-8):(2-5):(1-2...
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