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Method for detecting quality of traditional Chinese medicine indigowoad root granules

A quality inspection method, the technology of Banlangen granules, applied in the field of natural medicinal chemistry, can solve the problems of not being able to control the quality of Banlangen granules as a whole, and achieve the effect of ensuring scientificity and advancement

Active Publication Date: 2010-12-15
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Banlangen Granules is a traditional Chinese medicine preparation recorded in the Pharmacopoeia of the People's Republic of China. It has the effects of clearing heat and detoxifying, cooling blood and relieving throat, and is used to treat sore throat, dry mouth and throat, and swelling of the cheeks caused by excessive heat in the lung and stomach. , acute tonsillitis, mumps, etc. [1] , modern pharmacodynamic studies have shown that Banlangen granules have strong antiviral effects [2] At present, the quality control method of Banlangen granules is mainly based on the quantitative analysis of indirubin, adenosine and other components [3-5] , is not enough to control the quality of Banlangen Granules as a whole, as a commonly used clinical preparation, its authenticity is affected by many factors such as the source of medicinal materials, production technology, etc., so it is necessary to establish a scientific quality detection method to monitor the quality of Banlangen Granules Evaluation, (references: [1] Pharmacopoeia Committee of the People's Republic of China. Pharmacopoeia of the People's Republic of China [M]. Beijing: Chemical Industry Press, 2005: 487-488. [2] An Yiqiang, Jia Xiaobin, Chen Yan, etc. RP -HPLC Determination of the Content of Yichun in Banlangen Granules from Different Manufacturers[J]. Chinese Journal of Traditional Chinese Medicine, 2009, 24(4): 529-531. [3] Yan Yizhou, Zou Pinwen. Determination of Indigo in Banlangen Granules by HPLC Content of Yuhong[J]. Chinese Pharmacy, 2008, 19(3): 211-212. [4] Hou Yaming, Wang Yulin, Hu Yibing. Determination of adenosine content in Banlangen Granules by HPLC[J]. Hunan Journal of Traditional Chinese Medicine, 2005, 21(3): 112-114. [5] Chen Mao, Liang Huiyu, Yang Dongsheng, etc. HPLC Analysis of Adenosine in Banlangen Granules [J]. Guangzhou Pharmacy, 2003, 13(6): 6 -8.)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Determination of Electrospray Fingerprint of Banlangen Granules

[0020] ①Preparation of the sample solution of Banlangen granules for testing

[0021] Take Radix isatidis granule as raw material, weigh 2g of Radix isatidis granule, add 10mL of water to dissolve, put it in a 10mL volumetric flask after drying, add 50% (V / V) methanol to dilute to 10mL, and filter to obtain the test solution;

[0022] ②Electrospray fingerprint of the test sample of Radix isatidis granules

[0023] The electrospray fingerprint of the test sample is obtained by the following steps: take 5 μL of the test solution, dilute it to 100 μL with methanol, and perform electrospray mass spectrometry analysis. The experimental conditions of electrospray mass spectrometry are as follows: electrospray ionization source, positive ionization mode, spray The voltage is 4.0kV, the capillary temperature is 180°C, the injection flow rate of the flow injection pump is 3μL / min, the scanning range mass-to-c...

Embodiment 2

[0031] (1) Determination of Electrospray Fingerprint of Banlangen Granules

[0032] ①Preparation of the sample solution of Banlangen granules for testing

[0033] Take Radix isatidis granules as raw material, weigh 4g of Radix isatidis granules, add 20mL of water to dissolve them, put them in a 10mL volumetric flask after drying, add 50% (V / V) methanol to dilute to 10mL, and filter to obtain the test solution;

[0034] ②Electrospray fingerprint of the test sample of Radix isatidis granules

[0035]The electrospray fingerprint of the test sample is obtained by the following steps: take 6 μL of the test solution, dilute it to 200 μL with methanol, and perform electrospray mass spectrometry analysis. The experimental conditions of electrospray mass spectrometry are as follows: electrospray ionization source, positive ionization mode, spray The voltage is 4.2kV, the capillary temperature is 250°C, the injection flow rate of the flow injection pump is 6μL / min, the scanning range m...

Embodiment 3

[0043] (1) Determination of Electrospray Fingerprint of Banlangen Granules

[0044] ①Preparation of the sample solution of Banlangen granules for testing

[0045] Take Radix isatidis granules as raw material, weigh 6g of Radix isatidis granules, add 30mL of water to dissolve them, put them in a 10mL volumetric flask after drying, add 50% (V / V) methanol to dilute to 10mL, and filter to obtain the test solution;

[0046] ②Electrospray fingerprint of the test sample of Radix isatidis granules

[0047] The electrospray fingerprint of the test sample is obtained by the following steps: take 7 μL of the test solution, dilute it to 300 μL with methanol, and perform electrospray mass spectrometry analysis. The experimental conditions of electrospray mass spectrometry are as follows: electrospray ionization source, positive ionization mode, spray The voltage is 4.5kV, the capillary temperature is 250°C, the injection flow rate of the flow injection pump is 6μL / min, the scanning range ...

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Abstract

The invention provides a method for detecting the quality of traditional Chinese medicine indigowoad root granules. The method comprises the following steps of: detecting the traditional Chinese medicine indigowoad root granules by using a soft-ionization mass spectrometry technique; performing primary evaluation on the number and strength of characteristic fingerprint peaks in an electro-spray fingerprint; measuring by high performance liquid chromatography to obtain the peak area of a chromatographic peak corresponding to each of cytidine, adenine, uridine, guanosine and adenosine serving as standard reference substances; and measuring according to a standard curve to obtain the cytidine content, adenine content, uridine content, guanosine content and adenosine content of one gram of the traditional Chinese medicine indigowoad root granules: 0.234 to 0.238 milligrams of cytidine, 0.116 to 0.118 milligrams of adenine, 0.472 to 0.474 milligrams of uridine, 0.331 to 0.334 milligrams of guanosine and 0.447 to 0.450 milligrams of adenosine. The method has the advantages of ensuring the scientificity and progressiveness of the method for detecting the quality of the traditional Chinese medicine indigowoad root granules, along with qualitative and quantitative performance.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a quality detection method of traditional Chinese medicine isatidis granules. Background technique [0002] Banlangen Granules is a traditional Chinese medicine preparation recorded in the Pharmacopoeia of the People's Republic of China. It has the effects of clearing heat and detoxifying, cooling blood and relieving throat, and is used to treat sore throat, dry mouth and throat, and swelling of the cheeks caused by excessive heat in the lung and stomach. , acute tonsillitis, mumps, etc. [1] , modern pharmacodynamic studies have shown that Banlangen granules have strong antiviral effects [2] At present, the quality control method of Banlangen granules is mainly based on the quantitative analysis of indirubin, adenosine and other components [3-5] , is not enough to control the quality of Banlangen Granules as a whole, as a commonly used clinical preparation...

Claims

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Application Information

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IPC IPC(8): A61K36/19A61K36/315G01N30/72A61P11/04A61P31/12A61P31/14
Inventor 刘志强闫峻刘舒皮子凤宋凤瑞刘忠英
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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