Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa

Abstract

A process for extracting spinosads from fermentation broth of Saccharopolyspora spinosa, comprising the following steps: (1) pretreatment of fermentation broth; (2) soaking and extracting spinosads in high dielectric constant polar organic solvents, and carrying out Solid-liquid separation, collection of leaching clear liquid; (3) Vacuum concentration, volatilization and removal of high dielectric constant polar solvents to obtain spinosad concentrates; (4) Adding low dielectric constant or high-carbon alcohol extraction solvents for extraction , to obtain the loaded organic phase; (5) add acid water to the loaded organic phase for back extraction, and collect the back extraction phase; (6) volatilize to remove the residual extraction solvent in the back extraction phase, and adjust the pH value to 8.5~11.5 with NaOH solution, Precipitate the spinosyn, filter, wash the spinosyn precipitate with dilute lye for 1 to 3 times, and dry in vacuum to obtain the spinosyn powder. The total yield of the invention can reach more than 80%, and has the advantages of high yield, good quality, low cost, easy industrialization and the like.

Description

Technical field [0001] The present invention involves a process from extracting antibiotics from the fermentation solution, especially the process of extracting multi -bacterin from the extraction method from the extraction method from the spores of spores. Background technique [0002] Sipinosad (SPINOSYNS), also known as spinosyns, and polyromycin. SacCharopolyspora Spinosa ) The secondary metabolites produced by aerobic fermentation are large -ring endone antibiotics. The molecular structure contains a twenty -two yuan lactone ring, a rat lip sugar, and a Fullagamine.It is easy to dissolve in a variety of polar and non -polar organic solvents such as alcohol and ester.After the aerobic fermentation, the spines polymoglacular fermentation liquid contains two types of insecticidal activity, which mainly contain multiple bactericin A (85 ~ 90%) and D (10 ~ 15%), and also contain a small amount of B, C, E, E, F, H, J, K, L and other similar structures are similar.Multi -bactericin...

AI Technical Summary

Problems solved by technology

[0006] Although the resin adsorption extraction method has a good effect to a certain extent, such as high purity, the macroporous resin adsorption extraction process currently has small adsorption capacity, difficult desorption, small processing capacity, high equipment requirements, and many processes. It is difficult to adapt to the requirements of large-scale industrial production due to defects such as long time consumption and high cost

[0007] At present, in the prior art, there is no spinosad extraction process with high yield, good purity, low cost, easy operation and convenient industrial production.

Images